Invitrogen

MERTK Monoclonal Antibody (DS5MMER), APC-eFluor™ 780, eBioscience™

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Antibody Testing Data (1)
Published Figures (7)

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MERTK Antibody (47-5751-82) in Flow

C57BL/6 mouse resident peritoneal macrophages were stained with CD11b Monoclonal Antibody, eFluor 450 (Product # 48-0112-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4321-82) (left) or 0.25 µg of MerTK Monoclonal Antibody, APC-eFluor 780 (right). Cells in the monocyte gate were used for analysis.

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Product Details

47-5751-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS5MMER

Immunogen

FC-tagged full protein expressed in insect cells

View immunogen

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848373

Product Specific Information

Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines. In addition MerTK may function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies. MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium. This process can be enhanced by stimulation with LPS. MerTK can be used to help discriminate macrophages from dendritic cells. MerTK is often expressed on malignant cells and may be implicated in immune evasion.

The DS5MMER antibody will work on paraformaldehyde-fixed cells.

Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS5MMER antibody has been tested by flow cytometric analysis of resident peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Pop OT,Geng A,Flint E,Singanayagam A,Ercan C,Possamai L,Patel VC,Kuenzler P,Meier MA,Soysal S,Hruz P,Kollmar O,Tatham KC,Ward JK,Müllhaupt B,Weber A,Wendon J,Niess JH,Heim M,Semela D,Weston C,Antoniades CG,Terracciano LM,Triantafyllou E,Brenig RG,Bernsmeier C