Invitrogen

F4/80 Monoclonal Antibody (BM8), Pacific Blue™

1 Published Figure

12 References

View all (67) F4/80 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

FIGURE: 1 / 2

F4/80 Antibody (MF48028) in Flow

Fresh mouse splenocytes were stained using Pacific Blue™ of rat anti-mouse F4 / 80 antibody (clone: BM8). The negative control profiles represent unstained cells.

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Product Details

MF48028

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BM8

Immunogen

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373419

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation.

Immunity

Liu Z,Nailwal H,Rector J,Rahman MM,Sam R,McFadden G,Chan FK

MF48028 was used in Flow Cytometry to identify a viral inhibitor that binds to the host SKP1-Cullin1-F-box (SCF) machinery and the essential necroptosis kinase receptor interacting protein kinase 3 (RIPK3).

Tue Feb 09 00:00:00 EST 2021

The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis.

Cell reports

Hou X,Chen G,Bracamonte-Baran W,Choi HS,Diny NL,Sung J,Hughes D,Won T,Wood MK,Talor MV,Hackam DJ,Klingel K,Davogustto G,Taegtmeyer H,Coppens I,Barin JG,Čiháková D

MF48028 was used in Flow cytometry/Cell sorting to propose that MHCII+Ly6Clo MDMs are associated with the reduction of cardiac fibrosis and prevention of the myocarditis sequalae.

Tue Jul 02 00:00:00 EDT 2019

LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux.

Cell reports

Thomas DG,Doran AC,Fotakis P,Westerterp M,Antonson P,Jiang H,Jiang XC,Gustafsson JÅ,Tabas I,Tall AR

MF48028 was used in Flow cytometry/Cell sorting to study the mechanisms underlying the repressive activity of liver X receptor.

Wed Dec 26 00:00:00 EST 2018

Serum Stability and Affinity Optimization of an M2 Macrophage-Targeting Peptide (M2pep).

Theranostics

Ngambenjawong C,Gustafson HH,Pineda JM,Kacherovsky NA,Cieslewicz M,Pun SH

MF48028 was used in flow cytometry to assess the serum stability and binding affinity of M2 macrophage-targeting peptide derivatives

Mon Oct 30 00:00:00 EDT 2017

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

MF48028 was used in flow cytometry and immunohistochemistry - frozen section to investigate how heme regulates Spic in macrophages.

Thu Mar 13 00:00:00 EDT 2014

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

MF48028 was used in flow cytometry and immunohistochemistry - frozen section to investigate how heme regulates Spic in macrophages.

Thu Mar 13 00:00:00 EDT 2014

Dendritic cells promote macrophage infiltration and comprise a substantial proportion of obesity-associated increases in CD11c+ cells in adipose tissue and liver.

Diabetes

Stefanovic-Racic M,Yang X,Turner MS,Mantell BS,Stolz DB,Sumpter TL,Sipula IJ,Dedousis N,Scott DK,Morel PA,Thomson AW,O'Doherty RM

MF48028 was used in Flow cytometry/Cell sorting to study the role of dendritic cells in obesity by mediating macrophage infiltration of adipose tissue and liver, the determination of tissue immunophenotype, and in systemic metabolic responses to a high-fat diet.

Sat Sep 01 00:00:00 EDT 2012

SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages.

Nature immunology

Berger SB,Romero X,Ma C,Wang G,Faubion WA,Liao G,Compeer E,Keszei M,Rameh L,Wang N,Boes M,Regueiro JR,Reinecker HC,Terhorst C

MF48028 was used in flow cytometry to report that SLAM is a microbial sensor aids in the killing of gram-negative bacteria by macrophages

Fri Oct 01 00:00:00 EDT 2010

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

MF48028 was used in flow cytometry to track populations of T cells specific for a given peptide-major histocompatibility complex epitope

Tue Jun 30 00:00:00 EDT 2009

Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Mangan NE,van Rooijen N,McKenzie AN,Fallon PG

MF48028 was used in flow cytometry to elucidate the mechanisms that regulate the inverse relationship between helminth infections and allergies

Sun Jan 01 00:00:00 EST 2006

Effect of thermal injury on splenic myelopoiesis.

Shock (Augusta, Ga.)

Noel JG,Guo X,Wells-Byrum D,Schwemberger S,Caldwell CC,Ogle CK

MF48028 was used in flow cytometry to measure the number of splenic myeloid progenitors and their progeny after burn injury

Tue Feb 01 00:00:00 EST 2005

A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes.

Cell and tissue research

Malorny U,Michels E,Sorg C

MF48028 was used in immunohistochemistry - frozen section to generate and characterize a monoclonal antibody raised in the rat against cultured mouse bone marrow-derived macrophages

Tue Apr 22 00:00:00 EST 1986

Bioinformatics

Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

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Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

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UniProt ID: (Mouse) Q61549

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Entrez Gene ID: (Mouse) 13733

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Function(s)

signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding G-protein coupled receptor

Process(es)

signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway

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F4/80 Monoclonal Antibody (BM8), Pacific Blue™

F4/80 Antibody (MF48028) in Flow

Product Details

MF48028

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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