Invitrogen

F4/80 Monoclonal Antibody (BM8), Pacific Orange™

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Details

MF48030

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BM8

Immunogen

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Concentration

0.3 mg/mL

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539704

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

TIM-3 regulates innate immune cells to induce fetomaternal tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Chabtini L,Mfarrej B,Mounayar M,Zhu B,Batal I,Dakle PJ,Smith BD,Boenisch O,Najafian N,Akiba H,Yagita H,Guleria I

MF48030 was used in flow cytometry to determine the role of TIM-3-expressing innate immune cells in the regulation of tolerance at the fetomaternal interface.

Tue Jan 01 00:00:00 EST 2013

IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia.

Nature immunology

Wang Y,Szretter KJ,Vermi W,Gilfillan S,Rossini C,Cella M,Barrow AD,Diamond MS,Colonna M

MF48030 was used in flow cytometry to report that IL-34 regulates differentiation of myeloid cells in the skin epidermis and CNS.

Sun Jun 24 00:00:00 EDT 2012

CD28 promotes CD4+ T cell clonal expansion during infection independently of its YMNM and PYAP motifs.

Journal of immunology (Baltimore, Md. : 1950)

Pagán AJ,Pepper M,Chu HH,Green JM,Jenkins MK

MF48030 was used in flow cytometry to investigate which portions of CD28 are needed for T cells.

Sat Sep 15 00:00:00 EDT 2012

Resistance and augmentation of innate immunity in mice exposed to starvation.

Cellular immunology

Shen J,Ren H,Tomiyama-Miyaji C,Watanabe M,Kainuma E,Inoue M,Kuwano Y,Abo T

MF48030 was used in flow cytometry to examine immune cell population 3 days after starvation.

Tue Sep 08 00:00:00 EDT 2009

Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Mangan NE,van Rooijen N,McKenzie AN,Fallon PG

MF48030 was used in flow cytometry to elucidate the mechanisms that regulate the inverse relationship between helminth infections and allergies

Sun Jan 01 00:00:00 EST 2006

Effect of thermal injury on splenic myelopoiesis.

Shock (Augusta, Ga.)

Noel JG,Guo X,Wells-Byrum D,Schwemberger S,Caldwell CC,Ogle CK

MF48030 was used in flow cytometry to measure the number of splenic myeloid progenitors and their progeny after burn injury

Tue Feb 01 00:00:00 EST 2005

A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes.

Cell and tissue research

Malorny U,Michels E,Sorg C

MF48030 was used in immunohistochemistry - frozen section to generate and characterize a monoclonal antibody raised in the rat against cultured mouse bone marrow-derived macrophages

Tue Apr 22 00:00:00 EST 1986

Bioinformatics

Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

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Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

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UniProt ID: (Mouse) Q61549

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Entrez Gene ID: (Mouse) 13733

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Function(s)

signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding G-protein coupled receptor

Process(es)

signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

F4/80 Monoclonal Antibody (BM8), Pacific Orange™

Product Details

MF48030

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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