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Invitrogen
MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), FITC, eBioscience™
FIGURE: 1 / 5
MHC Class I (H-2Db) Antibody (11-5999-82) in Flow
Staining of mouse splenocytes with 0.25 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (blue histogram) or 0.25 µg of Anti-Mouse MHC Class I (H-2Db) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.
Product Details
11-5999-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
28-14-8
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_465361
Product Specific Information
Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.
Applications Reported: 28-14-8 has been reported for use in flow cytometric analysis.
Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D cell surface glycoprotein; H-2D(B); H2-DC1-beta transplantation antigen protein; H2D1; H2DB; H2L; H2LD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein
Gene Aliases: H-2D; H2-D; H2-D1
UniProt ID: (Mouse) P01899
Entrez Gene ID: (Mouse) 14964
positive regulation of T cell mediated cytotoxicity
immune system process
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
immune response
antigen processing and presentation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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