Invitrogen

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

14 References

View all (27) MHC Class I (H-2Db) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MHC Class I (H-2Db) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

MHC Class I (H-2Db) Antibody (12-5999-82) in Flow

Staining of mouse splenocytes with 0.25 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or 0.25 µg of Anti-Mouse MHC Class I (H-2Db) PE (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-5999-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

28-14-8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466125

Product Specific Information

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 12-5999-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

12-5999 was used in Flow cytometry/Cell sorting to provide genetic evidence supporting that NKG2A and the inhibitory members of Ly49 family receptors synergize to regulate NK cell education.

Fri Nov 01 00:00:00 EDT 2019

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 12-5999-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

TCR stimulation strength is inversely associated with establishment of functional brain-resident memory CD8 T cells during persistent viral infection.

PLoS pathogens

Maru S,Jin G,Schell TD,Lukacher AE

12-5999 was used in Flow cytometry/Cell sorting to assess the impact of antigen stimulation strength on memory CD8 T cell formation and function during a persistent viral infection.

Sat Apr 01 00:00:00 EDT 2017

The Shc1 adaptor simultaneously balances Stat1 and Stat3 activity to promote breast cancer immune suppression.

Nature communications

Ahn R,Sabourin V,Bolt AM,Hébert S,Totten S,De Jay N,Festa MC,Young YK,Im YK,Pawson T,Koromilas AE,Muller WJ,Mann KK,Kleinman CL,Ursini-Siegel J

12-5999 was used in Flow cytometry/Cell sorting to elucidate the mechanisms behind Shc1-driven breast cancer immune suppression.

Thu Mar 09 00:00:00 EST 2017

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

12-5999 was used in Flow cytometry/Cell sorting to determine that MSCs may be safe and effective when used in allogeneic BMT for the treatment of haemotological malignancies.

Wed Jul 01 00:00:00 EDT 2015

Autophagy regulates the therapeutic potential of mesenchymal stem cells in experimental autoimmune encephalomyelitis.

Autophagy

Dang S,Xu H,Xu C,Cai W,Li Q,Cheng Y,Jin M,Wang RX,Peng Y,Zhang Y,Wu C,He X,Wan B,Zhang Y

12-5999 was used in Flow cytometry/Cell sorting to study how inflammatory microenvironment-induced autophagy can downregulate the immunosuppressive function of mesenchymal stem cells.

Tue Jul 01 00:00:00 EDT 2014

γδ T cells recognize the insulin B:9-23 peptide antigen when it is dimerized through thiol oxidation.

Molecular immunology

Aydintug MK,Zhang L,Wang C,Liang D,Wands JM,Michels AW,Hirsch B,Day BJ,Zhang G,Sun D,Eisenbarth GS,O'Brien RL,Born WK

12-5999 was used in Flow cytometry/Cell sorting to determine why γδ T cells require a secondary amino acid recognition site on the insulin peptide, B:9-23.

Fri Aug 01 00:00:00 EDT 2014

Rhamnogalacturonan II is a Toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells.

Experimental & molecular medicine

Park SN,Noh KT,Jeong YI,Jung ID,Kang HK,Cha GS,Lee SJ,Seo JK,Kang DH,Hwang TH,Lee EK,Kwon B,Park YM

12-5999 was used in Flow cytometry/Cell sorting to evaluate the effectiveness of RG-II-stimulated BMDC vaccination on the induction of antitumor immunity, showing that it inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells.

Fri Feb 08 00:00:00 EST 2013

Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumour necrosis factor alpha and interleukin 6 production in murine macrophages.

Journal of medical microbiology

Daim S,Kawamura I,Tsuchiya K,Hara H,Kurenuma T,Shen Y,Dewamitta SR,Sakai S,Nomura T,Qu H,Mitsuyama M

12-5999 was used in Flow cytometry/Cell sorting to suggest a potential role of PPE37 in interfering with the pro-inflammatory cytokine response of infected macrophages.

Sun May 01 00:00:00 EDT 2011

Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase.

Journal of immunology (Baltimore, Md. : 1950)

Nice TJ,Deng W,Coscoy L,Raulet DH

12-5999 was used in Flow cytometry/Cell sorting to investigate the regulation of cell surface expression of the NKG2D ligand, Mult1.

Mon Nov 01 00:00:00 EDT 2010

Deficient major histocompatibility complex-linked innate murine cytomegalovirus immunity in MA/My.L-H2b mice and viral downregulation of H-2k class I proteins.

Journal of virology

Xie X,Dighe A,Clark P,Sabastian P,Buss S,Brown MG

12-5999 was used in Flow cytometry/Cell sorting to show that replacement of MA/My H-2k with C57L H-2b susceptibility genes led to a remarkable loss of innate virus immunity.

Mon Jan 01 00:00:00 EST 2007

CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells.

International immunology

Kuwano Y,Prazma CM,Yazawa N,Watanabe R,Ishiura N,Kumanogoh A,Okochi H,Tamaki K,Fujimoto M,Tedder TF

12-5999 was used in Flow cytometry/Cell sorting to investigate the role of CD83 in B cells and antigen-presenting cells.

Wed Aug 01 00:00:00 EDT 2007

Isolation and characterization of kidney-derived stem cells.

Journal of the American Society of Nephrology : JASN

Gupta S,Verfaillie C,Chmielewski D,Kren S,Eidman K,Connaire J,Heremans Y,Lund T,Blackstad M,Jiang Y,Luttun A,Rosenberg ME

12-5999 was used in Immunohistochemistry to study how MRPC participate in the regenerative response of the kidney to acute injury.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D cell surface glycoprotein; H-2D(B); H2-DC1-beta transplantation antigen protein; H2D1; H2DB; H2L; H2LD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein

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Gene Aliases: H-2D; H2-D; H2-D1

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UniProt ID: (Mouse) P01899

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Entrez Gene ID: (Mouse) 14964

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), PE, eBioscience™

MHC Class I (H-2Db) Antibody (12-5999-82) in Flow

Product Details

12-5999-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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