Invitrogen

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Functional Grade, eBioscience™

MHC Class I (H-2Db) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Db) Antibody (16-5999-82) in Flow

Oncogene 2019 - Figure 6. CXCL14-mediated tumor suppression is abrogated by MHC-I knockdown in HNC cells. MOE/E6E7 CXCL14 cells were transduced with shRNAs directed to B2M and enriched for knockdown by FACS sorting for the bottom 10% of MHC-I expressing cells (MOE/E6E7 CXCL14/shB2M ). Flow cytometric analysis of H-2D b (A) and H-2K b (B) MHC-I molecules in MOE/E6E7 Vector (gray), MOE/E6E7 CXCL14 (blue), and MOE/E6E7 ... View More

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Product Details

16-5999-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Functional Assay (Functional)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

28-14-8

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469197

Product Specific Information

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tnfrsf4-expressing regulatory T cells promote immune escape of chronic myeloid leukemia stem cells.

JCI insight

Hinterbrandner M,Rubino V,Stoll C,Forster S,Schnüriger N,Radpour R,Baerlocher GM,Ochsenbein AF,Riether C

16-5999-82 was used in Flow Cytometry to identify Tregs as key regulators of immune escape of leukemia stem cells and tumour necrosis factor receptor 4 as a potential target to reduce the function of Tregs and boost antileukemic immunity in chronic myeloid leukemia.

Wed Dec 08 00:00:00 EST 2021

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 16-5999-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 16-5999-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

The Shc1 adaptor simultaneously balances Stat1 and Stat3 activity to promote breast cancer immune suppression.

Nature communications

Ahn R,Sabourin V,Bolt AM,Hébert S,Totten S,De Jay N,Festa MC,Young YK,Im YK,Pawson T,Koromilas AE,Muller WJ,Mann KK,Kleinman CL,Ursini-Siegel J

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 16-5999-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

N-glycosylation converts non-glycoproteins into mannose receptor ligands and reveals antigen-specific T cell responses in vivo.

Oncotarget

Kreer C,Kuepper JM,Zehner M,Quast T,Kolanus W,Schumak B,Burgdorf S

16-5999 was used in Flow cytometry/Cell sorting to analyse whether enforced N-glycosylation is a suited strategy to enhance the immunogenicity of non-glycosylated antigens for vaccination studies.

Tue Jan 24 00:00:00 EST 2017

T Cell Priming by Activated Nlrc5-Deficient Dendritic Cells Is Unaffected despite Partially Reduced MHC Class I Levels.

Journal of immunology (Baltimore, Md. : 1950)

Rota G,Ludigs K,Siegert S,Tardivel A,Morgado L,Reith W,De Gassart A,Guarda G

16-5999 was used in Flow cytometry/Cell sorting to show that Nlrc5 deficiency is not sufficient to hinder T cell activation, highlighting the robustness of the T cell priming process by activated dendritic cells.

Fri Apr 01 00:00:00 EDT 2016

IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require α4β1 Integrin To Migrate between the Peritoneum and Inflamed Skin.

Journal of immunology (Baltimore, Md. : 1950)

Geherin SA,Gómez D,Glabman RA,Ruthel G,Hamann A,Debes GF

16-5999 was used in Flow cytometry/Cell sorting to investigate innate-like B cells within the skin.

Tue Mar 15 00:00:00 EDT 2016

Identification of Candidate Tolerogenic CD8(+) T Cell Epitopes for Therapy of Type 1 Diabetes in the NOD Mouse Model.

Journal of diabetes research

Yu C,Burns JC,Robinson WH,Utz PJ,Ho PP,Steinman L,Frey AB

16-5999 was used in Flow cytometry/Cell sorting to investigate two predicted tolerogenic CD8(+) T cell epitopes from the NOD murine model that are pathogenic in type 1 diabetes.

Tue Dec 13 00:00:00 EST 2016

Fine needle aspirate flow cytometric phenotyping characterizes immunosuppressive nature of the mesothelioma microenvironment.

Scientific reports

Lizotte PH,Jones RE,Keogh L,Ivanova E,Liu H,Awad MM,Hammerman PS,Gill RR,Richards WG,Barbie DA,Bass AJ,Bueno R,English JM,Bittinger M,Wong KK

16-5999 was used in Flow cytometry/Cell sorting to develop a fine needle aspirate biopsy platform to perform immune profiling on thoracic malignancies.

Fri Aug 19 00:00:00 EDT 2016

Adaptive Immune Regulation of Mammary Postnatal Organogenesis.

Developmental cell

Plaks V,Boldajipour B,Linnemann JR,Nguyen NH,Kersten K,Wolf Y,Casbon AJ,Kong N,van den Bijgaart RJ,Sheppard D,Melton AC,Krummel MF,Werb Z

16-5999 was used in Inhibition experiments to show that adaptive immune responses participate in the normal postnatal development of a non-lymphoid epithelial tissue.

Mon Sep 14 00:00:00 EDT 2015

Intratumoral IL-12 combined with CTLA-4 blockade elicits T cell-mediated glioma rejection.

The Journal of experimental medicine

Vom Berg J,Vrohlings M,Haller S,Haimovici A,Kulig P,Sledzinska A,Weller M,Becher B

16-5999 was used in Flow cytometry/Cell sorting to study intratumoral elicitors of T-cell mediated glioma rejection.

Mon Dec 16 00:00:00 EST 2013

Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Nouri-Shirazi M,Guinet E

16-5999 was used in Flow cytometry/Cell sorting to investigate the causal relationship between nicotine-induced dendritic cell alterations and host response to vaccines.

Thu Mar 01 00:00:00 EST 2012

CSF-1-dependent red pulp macrophages regulate CD4 T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Kurotaki D,Kon S,Bae K,Ito K,Matsui Y,Nakayama Y,Kanayama M,Kimura C,Narita Y,Nishimura T,Iwabuchi K,Mack M,van Rooijen N,Sakaguchi S,Uede T,Morimoto J

16-5999 was used in Flow cytometry/Cell sorting to show tissue macrophages residing in the red pulp showed different expression patterns of surface markers compared with F4/80(+)Mac-1(hi) monocytes.

Tue Feb 15 00:00:00 EST 2011

Effective melanoma immunotherapy in mice by the skin-depigmenting agent monobenzone and the adjuvants imiquimod and CpG.

PloS one

van den Boorn JG,Konijnenberg D,Tjin EP,Picavet DI,Meeuwenoord NJ,Filippov DV,van der Veen JP,Bos JD,Melief CJ,Luiten RM

16-5999 was used in Flow cytometry/Cell sorting to demonstrate that MIC therapy is effective in eradicating melanoma, by incorporating NK-, B- and T-cells in its therapeutic effect.

Thu May 13 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D cell surface glycoprotein; H-2D(B); H2-DC1-beta transplantation antigen protein; H2D1; H2DB; H2L; H2LD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein

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Gene Aliases: H-2D; H2-D; H2-D1

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UniProt ID: (Mouse) P01899

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Entrez Gene ID: (Mouse) 14964

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), Functional Grade, eBioscience™

MHC Class I (H-2Db) Antibody (16-5999-82) in Flow

Product Details

16-5999-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Control (Ctrl)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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