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FIGURE: 1 / 3
Mouse IgG3 Isotype Control (14-4742-82) in ICC/IF
Immunocytochemistry of fixed A375 cells using 5 µg/mL of Mouse IgG3 Isotype Control Purified (left) or 5 µg/mL of Anti-Human Disialoganglioside GD3 Purified (right) followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
Product Details
14-4742-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
Assay-Dependent
Immunocytochemistry (ICC/IF)
Assay-Dependent
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG3, lambda
Class
Monoclonal
Type
Isotype Control
Clone
B10
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_470120
Product Specific Information
Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG3 isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use the isotype control at the same concentration as the experimental antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG3 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the least abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG3 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG3 is less commonly used in research and diagnostic applications compared to other IgG subtypes, but it is still used in some applications such as ELISA and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G3; ImmunoglobulinG; ImmunoglobulinG3
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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