Invitrogen

Myosin 4 Monoclonal Antibody (MF20), eFluor™ 660, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

13 Published Figures

16 References

View all (9) Myosin 4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Myosin 4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Myosin 4 Antibody (50-6503-82) in ICC/IF

Immunofluorescence analysis of MYH4 was performed using 70% confluent log phase C2C12 cells and C2C12 cells differentiated to Myotubes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myosin 4 Monoclonal Antibody (MF20), eFluor 660, eBioscience™ (Product # 50-6503-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L)... View More

Myosin 4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Myosin 4 Antibody in Immunohistochemistry (IHC)

Myosin 4 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

50-6503-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 8 publications 8 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Chimpanzee, Cat, Guinea pig, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MF20

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574267

Product Specific Information

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.

The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.

Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This MF20 antibody has been tested immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice.

Cell death & disease

Al-Zaeed N,Budai Z,Szondy Z,Sarang Z

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Western Blot

Sat Jun 12 00:00:00 EDT 2021

In vivo detection of programmed cell death during mouse heart development.

Cell death and differentiation

Martínez-Lagunas K,Yamaguchi Y,Becker C,Geisen C,DeRuiter MC,Miura M,Fleischmann BK,Hesse M

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice.

Scientific reports

Ono Y,Maejima Y,Saito M,Sakamoto K,Horita S,Shimomura K,Inoue S,Kotani J

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Western Blot

Mon Jan 20 00:00:00 EST 2020

TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice.

Scientific reports

Ono Y,Maejima Y,Saito M,Sakamoto K,Horita S,Shimomura K,Inoue S,Kotani J

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Western Blot

Mon Jan 20 00:00:00 EST 2020

Data on the proliferation and differentiation of C2C12 myoblast treated with branched-chain ketoacid dehydrogenase kinase inhibitor.

Data in brief

Sato Y,Tate H,Yoshizawa F,Sato Y

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Western Blot

Sat Aug 01 00:00:00 EDT 2020

hiPSC-Derived Neurons Provide a Robust and Physiologically Relevant In Vitro Platform to Test Botulinum Neurotoxins.

Frontiers in pharmacology

Lamotte JD,Roqueviere S,Gautier H,Raban E,Bouré C,Fonfria E,Krupp J,Nicoleau C

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Immunocytochemistry

Tue Feb 02 00:00:00 EST 2021

Myocardial protection by nanomaterials formulated with CHIR99021 and FGF1.

JCI insight

Fan C,Oduk Y,Zhao M,Lou X,Tang Y,Pretorius D,Valarmathi MT,Walcott GP,Yang J,Menasche P,Krishnamurthy P,Zhu W,Zhang J

50-6503-82 was used in Immunohistochemistry (Frozen), Immunocytochemistry to reveal that CHIR + FGF1-NPs resulted in a reduction of cardiomyocyte apoptosis and increase of angiogenesis.

Thu Jun 18 00:00:00 EDT 2020

Myocardial protection by nanomaterials formulated with CHIR99021 and FGF1.

JCI insight

Fan C,Oduk Y,Zhao M,Lou X,Tang Y,Pretorius D,Valarmathi MT,Walcott GP,Yang J,Menasche P,Krishnamurthy P,Zhu W,Zhang J

50-6503-82 was used in Immunohistochemistry (Frozen), Immunocytochemistry to reveal that CHIR + FGF1-NPs resulted in a reduction of cardiomyocyte apoptosis and increase of angiogenesis.

Thu Jun 18 00:00:00 EDT 2020

Peptidyl-prolyl cis-trans isomerase NIMA interacting 1 regulates skeletal muscle fusion through structural modification of Smad3 in the linker region.

Journal of cellular physiology

Islam R,Yoon H,Shin HR,Bae HS,Kim BS,Yoon WJ,Woo KM,Baek JH,Lee YS,Ryoo HM

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Immunocytochemistry

Sat Dec 01 00:00:00 EST 2018

Expression and localisation of thymosin beta-4 in the developing human early fetal heart.

PloS one

Saunders V,Dewing JM,Sanchez-Elsner T,Wilson DI

50-6503 was used in Immunohistochemistry to investigate the expression of Tβ4 in the developing human heart and demonstrate that Tβ4 in the human heart is primarily localised to endothelial cells of the cardiac microvasculature and coronary vessels as-well as to the endothelial-like cells of the endocardium and to the epicardium.

Mon Apr 22 00:00:00 EDT 2019

Ultrastructure of medial rectus muscles in patients with intermittent exotropia.

Eye (London, England)

Yao J,Wang X,Ren H,Liu G,Lu P

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Western Blot

Fri Jan 01 00:00:00 EST 2016

Single-molecule analysis of myocyte differentiation reveals bimodal lineage commitment.

Integrative biology : quantitative biosciences from nano to macro

Gibson TM,Gersbach CA

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Immunofluorescence

Mon Jun 01 00:00:00 EDT 2015

Photoluminescent Mesoporous Silicon Nanoparticles with siCCR2 Improve the Effects of Mesenchymal Stromal Cell Transplantation after Acute Myocardial Infarction.

Theranostics

Lu W,Xie Z,Tang Y,Bai L,Yao Y,Fu C,Ma G

50-6503 was used in Immunofluorescence to exhibit a new strategy to enhance mesenchymal stromal cell survival and inhibit cardiac remodelling in infarcted myocardium.

Mon Apr 25 00:00:00 EDT 2016

iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes.

Circulation. Cardiovascular genetics

Martinez-Fernandez A,Nelson TJ,Reyes S,Alekseev AE,Secreto F,Perez-Terzic C,Beraldi R,Sung HK,Nagy A,Terzic A

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Inhibition of TGFβ signaling increases direct conversion of fibroblasts to induced cardiomyocytes.

PloS one

Ifkovits JL,Addis RC,Epstein JA,Gearhart JD

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Immunofluorescence

Tue Jan 20 00:00:00 EST 2015

Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success.

Journal of molecular and cellular cardiology

Addis RC,Ifkovits JL,Pinto F,Kellam LD,Esteso P,Rentschler S,Christoforou N,Epstein JA,Gearhart JD

Published figure using Myosin 4 monoclonal antibody (Product # 50-6503-82) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 2B myosin heavy chain; fast myosin heavy chain 2B; myh4; MyHC IIb isoform; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; myosin heavy chain 2X; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4; unnamed protein product

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Gene Aliases: AI506973; MHC; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf

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UniProt ID: (Human) Q9Y623, (Dog) Q076A5, (Rabbit) Q28641, (Mouse) Q5SX39, (Rat) Q29RW1

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Entrez Gene ID: (Human) 4622, (Bovine) 408020, (Dog) 479502, (Rabbit) 100009548, (Rabbit) 100125991, (Guinea pig) 100726456, (Mouse) 17884, (Rat) 360543

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Function(s)

microfilament motor activity double-stranded RNA binding actin binding calmodulin binding ATP binding ATPase activity motor activity actin filament binding nucleotide binding molecular_function

Process(es)

muscle contraction response to activity actin filament-based movement muscle filament sliding ATP metabolic process metabolic process response to gravity response to muscle activity response to muscle stretch muscle structure development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Myosin 4 Monoclonal Antibody (MF20), eFluor™ 660, eBioscience™

Myosin 4 Antibody (50-6503-82) in ICC/IF

Product Details

50-6503-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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