Invitrogen

Perforin Monoclonal Antibody (dG9 (delta G9)), FITC, eBioscience™

Perforin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

Perforin Antibody (11-9994-42) in Flow

Intracellular staining of normal human peripheral blood cells with Anti-Human CD8 APC-eFluor® 780 (Product # 47-0088-42) and Mouse IgG2b K Isotype Control FITC (Product # 11-4732-42) (left) or Anti-Human Perforin FITC (right). Cells in the lymphocyte gate were used for analysis.

Perforin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-9994-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

dG9 (delta G9)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1944474

Product Specific Information

Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistochemical staining.

Applications Tested: This dG9 (delta G9) antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In vitro vascular differentiation system efficiently produces natural killer cells for cancer immunotherapies.

Oncoimmunology

Galat Y,Du Y,Perepitchka M,Li XN,Balyasnikova IV,Tse WT,Dambaeva S,Schneiderman S,Iannaccone PM,Becher O,Graham DK,Galat V

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2023

CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance.

Cancer immunology research

Lao L,Zeng W,Huang P,Chen H,Jia Z,Wang P,Huang D,Chen J,Nie Y,Yang L,Wu W,Liu J

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes.

JCI insight

Zhang M,Chong KK,Chen ZY,Guo H,Liu YF,Kang YY,Li YJ,Shi TT,Lai KK,He MQ,Ye K,Kahaly GJ,Shi BY,Wang Y

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Wed Feb 08 00:00:00 EST 2023

Neoadjuvant chemotherapy plus nivolumab with or without ipilimumab in operable non-small cell lung cancer: the phase 2 platform NEOSTAR trial.

Nature medicine

Cascone T,Leung CH,Weissferdt A,Pataer A,Carter BW,Godoy MCB,Feldman H,William WN,Xi Y,Basu S,Sun JJ,Yadav SS,Rojas Alvarez FR,Lee Y,Mishra AK,Chen L,Pradhan M,Guo H,Sinjab A,Zhou N,Negrao MV,Le X,Gay CM,Tsao AS,Byers LA,Altan M,Glisson BS,Fossella FV,Elamin YY,Blumenschein G,Zhang J,Skoulidis F,Wu J,Mehran RJ,Rice DC,Walsh GL,Hofstetter WL,Rajaram R,Antonoff MB,Fujimoto J,Solis LM,Parra ER,Haymaker C,Wistuba II,Swisher SG,Vaporciyan AA,Lin HY,Wang J,Gibbons DL,Jack Lee J,Ajami NJ,Wargo JA,Allison JP,Sharma P,Kadara H,Heymach JV,Sepesi B

11-9994-42 was used in Flow Cytometry, Immunohistochemistry to report the results and correlates of two arms of the phase 2 platform NEOSTAR trial testing neoadjuvant Nivo+CT and Ipi+Nivo+CT with major pathologic response (MPR) as the primary endpoint.

Wed Mar 01 00:00:00 EST 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

11-9994-42 was used in Flow Cytometry to demonstrate that LINC01123 acts as a miR-214-3p sponge to inhibit the activation of CD8+T cells and promote the progression of HNSCC by upregulating B7-H3.

Thu Feb 03 00:00:00 EST 2022

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

11-9994-42 was used in Flow Cytometry to demonstrate that LINC01123 acts as a miR-214-3p sponge to inhibit the activation of CD8+T cells and promote the progression of HNSCC by upregulating B7-H3.

Thu Feb 03 00:00:00 EST 2022

Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.

Nature communications

Liu X,Jin S,Hu S,Li R,Pan H,Liu Y,Lai P,Xu D,Sun J,Liu Z,Gao Y,Zhao Y,Liu F,Xiao Y,Li Y,Wen Y,Chen Z,Xu B,Lin Y,Ran M,Li Q,Yang S,Li H,Tu P,Haniffa M,Teichmann SA,Bai F,Wang Y

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Immunohistochemistry

Thu Mar 03 00:00:00 EST 2022

PD-1-Expressing SARS-CoV-2-Specific CD8⁺ T Cells Are Not Exhausted, but Functional in Patients with COVID-19.

Immunity

Rha MS,Jeong HW,Ko JH,Choi SJ,Seo IH,Lee JS,Sa M,Kim AR,Joo EJ,Ahn JY,Kim JH,Song KH,Kim ES,Oh DH,Ahn MY,Choi HK,Jeon JH,Choi JP,Kim HB,Kim YK,Park SH,Choi WS,Choi JY,Peck KR,Shin EC

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Human Thymic CD10⁺ PD-1⁺ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a^- CD95⁺ CD28^- CCR7^- Developmental Stage.

International journal of molecular sciences

Billiet L,Goetgeluk G,Bonte S,De Munter S,De Cock L,Pille M,Ingels J,Jansen H,Weening K,Van Nieuwerburgh F,Kerre T,Taghon T,Leclercq G,Vandekerckhove B

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Fri Nov 20 00:00:00 EST 2020

Neutrophil expansion defines an immunoinhibitory peripheral and intratumoral inflammatory milieu in resected non-small cell lung cancer: a descriptive analysis of a prospectively immunoprofiled cohort.

Journal for immunotherapy of cancer

Mitchell KG,Diao L,Karpinets T,Negrao MV,Tran HT,Parra ER,Corsini EM,Reuben A,Federico L,Bernatchez C,Dejima H,Francisco-Cruz A,Wang J,Antonoff MB,Vaporciyan AA,Swisher SG,Cascone T,Wistuba II,Heymach JV,Gibbons DL,Zhang J,Haymaker CL,Sepesi B

11-9994 was used in Flow cytometry/Cell sorting to demonstrate that neutrophil expansion reflects protumorigenic and immunosuppressive processes that manifest as worse overall survival (OS) in patients undergoing non-small lung cancer (NSCLC) resection.

Wed Apr 01 00:00:00 EDT 2020

Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting.

Cancer cell

Rotolo A,Caputo VS,Holubova M,Baxan N,Dubois O,Chaudhry MS,Xiao X,Goudevenou K,Pitcher DS,Petevi K,Kachramanoglou C,Iles S,Naresh K,Maher J,Karadimitris A

11-9994 was used in Flow cytometry/Cell sorting to investigate whether chimeric antigen receptor anti-CD19-engineering of the CD1d-restricted invariant natural killer T cells would result in enhanced anti-lymphoma activity.

Mon Oct 08 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

The Transcription Factor Hobit Identifies Human Cytotoxic CD4⁺ T Cells.

Frontiers in immunology

Oja AE,Vieira Braga FA,Remmerswaal EB,Kragten NA,Hertoghs KM,Zuo J,Moss PA,van Lier RA,van Gisbergen KP,Hombrink P

11-9994 was used in Flow cytometry/Cell sorting to suggest a shared differentiation pathway in CD4+, CD8+, and γδ+ T cells that may involve Hobit-driven acquisition of long-lived cytotoxic effector function.

Mon Jan 31 00:00:00 EST 2022

Human Asymptomatic Epitopes Identified from the Herpes Simplex Virus Tegument Protein VP13/14 (UL47) Preferentially Recall Polyfunctional Effector Memory CD44high CD62Llow CD8+ TEM Cells and Protect Humanized HLA-A*02:01 Transgenic Mice against Ocular Herpesvirus Infection.

Journal of virology

Srivastava R,Khan AA,Garg S,Syed SA,Furness JN,Vahed H,Pham T,Yu HT,Nesburn AB,BenMohamed L

11-9994 was used in Flow cytometry/Cell sorting to outline the phenotypic and functional features of protective HSV-specific CD8 T cells that should guide the development of a safe and effective T-cell-based herpes simplex vaccine.

Sun Jan 15 00:00:00 EST 2017

Eomesodermin and T-bet mark developmentally distinct human natural killer cells.

JCI insight

Collins A,Rothman N,Liu K,Reiner SL

11-9994 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation.

Thu Mar 09 00:00:00 EST 2017

Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells.

Oncoimmunology

Seebauer CT,Brunner S,Glockzin G,Piso P,Ruemmele P,Schlitt HJ,Geissler EK,Fichtner-Feigl S,Kesselring R

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Ghosh S,Taylor A,Chin M,Huang HR,Conery AR,Mertz JA,Salmeron A,Dakle PJ,Mele D,Cote A,Jayaram H,Setser JW,Poy F,Hatzivassiliou G,DeAlmeida-Nagata D,Sandy P,Hatton C,Romero FA,Chiang E,Reimer T,Crawford T,Pardo E,Watson VG,Tsui V,Cochran AG,Zawadzke L,Harmange JC,Audia JE,Bryant BM,Cummings RT,Magnuson SR,Grogan JL,Bellon SF,Albrecht BK,Sims RJ,Lora JM

Fri Jun 17 00:00:00 EDT 2016

IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Oncoimmunology

Braun M,Ress ML,Yoo YE,Scholz CJ,Eyrich M,Schlegel PG,Wölfl M

11-9994 was used in Flow cytometry/Cell sorting to investigate how IL12, and other inflammatory cytokines, shape effector functions of human T-cells.

Fri Jul 01 00:00:00 EDT 2016

Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Béziat V,Nyström S,Hengst J,Ivarsson MA,Kekäläinen E,Johansson H,Mjösberg J,Westgren M,Lankisch TO,Wedemeyer H,Ellis EC,Ljunggren HG,Michaëlsson J,Björkström NK

11-9994 was used in Flow cytometry/Cell sorting to study the unique lineage of CD49a(+)DX5(-) NK cells, showing that they are readily detectable in the liver but not in hepatic venous or peripheral blood.

Sun Mar 15 00:00:00 EDT 2015

Differentiation and functional regulation of human fetal NK cells.

The Journal of clinical investigation

Ivarsson MA,Loh L,Marquardt N,Kekäläinen E,Berglin L,Björkström NK,Westgren M,Nixon DF,Michaëlsson J

11-9994 was used in Flow cytometry/Cell sorting to provide a basis for understanding the role of foetal NK cells in pregnancy complications.

Sun Sep 01 00:00:00 EDT 2013

Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma.

Science translational medicine

Barnig C,Cernadas M,Dutile S,Liu X,Perrella MA,Kazani S,Wechsler ME,Israel E,Levy BD

Published figure using Perforin monoclonal antibody (Product # 11-9994-42) in Immunofluorescence

Wed Feb 27 00:00:00 EST 2013

Transfer of regulatory properties from tolerogenic to proinflammatory dendritic cells via induced autoreactive regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kleijwegt FS,Laban S,Duinkerken G,Joosten AM,Koeleman BP,Nikolic T,Roep BO

11-9994 was used in Flow cytometry/Cell sorting to study the mechanism by which infectious tolerance applies to human dendritic cells.

Thu Dec 15 00:00:00 EST 2011

Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.

PloS one

Yu Y,Zitzner JR,Houlihan J,Herrera N,Xu L,Miller J,Mathew JM,Tambur AR,Luo X

11-9994 was used in Flow cytometry/Cell sorting to demonstrate the use of common γc cytokines in combination with donor APCs as a strategy for generation of donor-specific T cells for immunotherapy.

Mon Aug 27 00:00:00 EDT 2012

The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors.

Journal of immunology (Baltimore, Md. : 1950)

Burt BM,Plitas G,Zhao Z,Bamboat ZM,Nguyen HM,Dupont B,DeMatteo RP

11-9994 was used in Flow cytometry/Cell sorting to conclude that the human liver contains NK cell subsets which have reduced effector function, but under appropriate inflammatory conditions become potent killers.

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Cytolysin; lymphocyte pore forming protein; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; pore-forming protein; PRF1 (pore forming protein 1); RP11-710A11.3

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Gene Aliases: FLH2; HPLH2; P1; PFN1; PFP; PRF1

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UniProt ID: (Human) P14222

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Entrez Gene ID: (Human) 5551, (Pig) 396595

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Function(s)

calcium ion binding protein binding wide pore channel activity

Process(es)

immunological synapse formation defense response to tumor cell immune response to tumor cell apoptotic process cellular defense response cytolysis protein homooligomerization defense response to virus transmembrane transport

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Perforin Monoclonal Antibody (dG9 (delta G9)), FITC, eBioscience™

Perforin Antibody (11-9994-42) in Flow

Product Details

11-9994-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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