Invitrogen

Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), Biotin, eBioscience™

27 Published Figures

29 References

View all (70) Podoplanin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (27)

Podoplanin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 28

Podoplanin Antibody (13-5381-82) in Flow

Staining of the TE-71 cell line with 0.125 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 13-4914-81) (blue histogram) or 0.125 µg of Anti-Mouse Podoplanin Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

Podoplanin Antibody in Immunohistochemistry (IHC)

Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed), Flow Cytometry (ICC/IF, IHC (PFA), Flow)

Podoplanin Antibody in Immunocytochemistry (ICC/IF)

Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

Product Details

13-5381-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 12 publications 12 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Flow Cytometry (Flow)

0.25 µg/Test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Mouse, Rat

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio8.1.1 (8.1.1)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016716

Product Specific Information

Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.

Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis and immunohistochemistry.

Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of TE-71 cells. This can be used at less than or equal to 0.25 µg per million cells in a 100 µL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer.

Cancer immunology research

Lidström T,Cumming J,Gaur R,Frängsmyr L,Pateras IS,Mickert MJ,Franklin O,Forsell MNE,Arnberg N,Dongre M,Patthey C,Öhlund D

13-5381-82 was used in Flow cytometry/Cell sorting to show that gal 4 is involved in immune evasion and identify galectin 4 as a promising drug target for overcoming immunosuppression in PDAC.

Tue Jan 03 00:00:00 EST 2023

Dysregulation of interaction between LOX^high fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Theranostics

Chen Y,Zhang T,Yao F,Gao X,Li D,Fu S,Mao L,Liu F,Zhang X,Xu Y,Deng J,Li W,Fan G,Xiao C,Chen Y,Wang L,Guo W,Zhou B

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunohistochemistry

Thu Mar 10 00:00:00 EST 2022

Vascularized lymph node transplantation successfully reverses lymphedema and maintains immunity in a rat lymphedema model.

Bioengineering & translational medicine

Sakarya AH,Huang CW,Yang CY,Hsiao HY,Chang FC,Huang JJ

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunohistochemistry

Thu Sep 01 00:00:00 EDT 2022

Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity.

Nature immunology

Prados A,Onder L,Cheng HW,Mörbe U,Lütge M,Gil-Cruz C,Perez-Shibayama C,Koliaraki V,Ludewig B,Kollias G

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Journal of cell science

de Winde CM,Makris S,Millward LJ,Cantoral-Rebordinos JA,Benjamin AC,Martínez VG,Acton SE

13-5381-82 was used in Immunohistochemistry to show a multi-faceted FRC response to DCs, which requires CD44 and CD9 in addition to podoplanin.

Thu Jul 15 00:00:00 EDT 2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Journal of cell science

de Winde CM,Makris S,Millward LJ,Cantoral-Rebordinos JA,Benjamin AC,Martínez VG,Acton SE

13-5381-82 was used in Immunohistochemistry to show a multi-faceted FRC response to DCs, which requires CD44 and CD9 in addition to podoplanin.

Thu Jul 15 00:00:00 EDT 2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Journal of cell science

de Winde CM,Makris S,Millward LJ,Cantoral-Rebordinos JA,Benjamin AC,Martínez VG,Acton SE

13-5381-82 was used in Immunohistochemistry to show a multi-faceted FRC response to DCs, which requires CD44 and CD9 in addition to podoplanin.

Thu Jul 15 00:00:00 EDT 2021

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

Podoplanin drives dedifferentiation and amoeboid invasion of melanoma.

iScience

de Winde CM,George SL,Crosas-Molist E,Hari-Gupta Y,Arp AB,Benjamin AC,Millward LJ,Makris S,Carver A,Imperatore V,Martínez VG,Sanz-Moreno V,Acton SE

13-5381-82 was used in Flow Cytometry, Immunohistochemistry, Immunocytochemistry to define podoplanin as a functional biomarker for dedifferentiated invasive melanoma and a promising migrastatic therapeutic target.

Fri Sep 24 00:00:00 EDT 2021

Podoplanin drives dedifferentiation and amoeboid invasion of melanoma.

iScience

de Winde CM,George SL,Crosas-Molist E,Hari-Gupta Y,Arp AB,Benjamin AC,Millward LJ,Makris S,Carver A,Imperatore V,Martínez VG,Sanz-Moreno V,Acton SE

Fri Sep 24 00:00:00 EDT 2021

Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.

PLoS biology

Fujimoto N,He Y,D'Addio M,Tacconi C,Detmar M,Dieterich LC

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growth.

eLife

Thacker VV,Dhar N,Sharma K,Barrile R,Karalis K,McKinney JD

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunocytochemistry

Tue Nov 24 00:00:00 EST 2020

Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury.

JCI insight

Riemondy KA,Jansing NL,Jiang P,Redente EF,Gillen AE,Fu R,Miller AJ,Spence JR,Gerber AN,Hesselberth JR,Zemans RL

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Tue Mar 26 00:00:00 EDT 2019

Transcriptional profiling of breast cancer-associated lymphatic vessels reveals VCAM-1 as regulator of lymphatic invasion and permeability.

International journal of cancer

Dieterich LC,Kapaklikaya K,Cetintas T,Proulx ST,Commerford CD,Ikenberg K,Bachmann SB,Scholl J,Detmar M

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Fri Nov 15 00:00:00 EST 2019

Podoplanin is an important stromal prognostic marker in perihilar cholangiocarcinoma.

Oncology letters

Obulkasim H,Shi X,Wang J,Li J,Dai B,Wu P,Wang S,Wang X,Ding Y

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Cornea lymphatics drive the CD8⁺ T-cell response to herpes simplex virus-1.

Immunology and cell biology

Gurung HR,Carr MM,Carr DJ

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Stem cell function and stress response are controlled by protein synthesis.

Nature

Blanco S,Bandiera R,Popis M,Hussain S,Lombard P,Aleksic J,Sajini A,Tanna H,Cortés-Garrido R,Gkatza N,Dietmann S,Frye M

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Thu Jun 16 00:00:00 EDT 2016

Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers.

Journal of hepatology

Lua I,Li Y,Zagory JA,Wang KS,French SW,Sévigny J,Asahina K

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunohistochemistry

Sun May 01 00:00:00 EDT 2016

Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers.

Journal of hepatology

Lua I,Li Y,Zagory JA,Wang KS,French SW,Sévigny J,Asahina K

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunohistochemistry

Sun May 01 00:00:00 EDT 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Chronic lung injury by constitutive expression of activation-induced cytidine deaminase leads to focal mucous cell metaplasia and cancer.

PloS one

Kitamura J,Uemura M,Kurozumi M,Sonobe M,Manabe T,Hiai H,Date H,Kinoshita K

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunohistochemistry

Mon Mar 30 00:00:00 EDT 2015

Alveolar progenitor and stem cells in lung development, renewal and cancer.

Nature

Desai TJ,Brownfield DG,Krasnow MA

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Thu Mar 13 00:00:00 EDT 2014

Dendritic cells control fibroblastic reticular network tension and lymph node expansion.

Nature

Acton SE,Farrugia AJ,Astarita JL,Mourão-Sá D,Jenkins RP,Nye E,Hooper S,van Blijswijk J,Rogers NC,Snelgrove KJ,Rosewell I,Moita LF,Stamp G,Turley SJ,Sahai E,Reis e Sousa C

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Thu Oct 23 00:00:00 EDT 2014

Dendritic cells control fibroblastic reticular network tension and lymph node expansion.

Nature

Acton SE,Farrugia AJ,Astarita JL,Mourão-Sá D,Jenkins RP,Nye E,Hooper S,van Blijswijk J,Rogers NC,Snelgrove KJ,Rosewell I,Moita LF,Stamp G,Turley SJ,Sahai E,Reis e Sousa C

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Thu Oct 23 00:00:00 EDT 2014

IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.

The Journal of experimental medicine

Fleige H,Ravens S,Moschovakis GL,Bölter J,Willenzon S,Sutter G,Häussler S,Kalinke U,Prinz I,Förster R

13-5381 was used in Immunohistochemistry to identify distinct pathogen-dependent induction and maturation pathways for BALT formation.

Mon Apr 07 00:00:00 EDT 2014

Mesodermal mesenchymal cells give rise to myofibroblasts, but not epithelial cells, in mouse liver injury.

Hepatology (Baltimore, Md.)

Lua I,James D,Wang J,Wang KS,Asahina K

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Tue Jul 01 00:00:00 EDT 2014

C3 dysregulation due to factor H deficiency is mannan-binding lectin-associated serine proteases (MASP)-1 and MASP-3 independent in vivo.

Clinical and experimental immunology

Ruseva MM,Takahashi M,Fujita T,Pickering MC

Tue Apr 01 00:00:00 EDT 2014

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

Published figure using Podoplanin monoclonal antibody (Product # 13-5381-82) in Immunofluorescence

Sun Mar 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: aggrus; CTA-520D8.1; E11; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; Transmembrane glycoprotein E11

View more View less

Gene Aliases: Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha

View more View less

UniProt ID: (Mouse) Q62011

View more View less

Entrez Gene ID: (Mouse) 14726

View more View less

Function(s)

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity

Process(es)

cell morphogenesis lymphangiogenesis prostaglandin metabolic process water transport amino acid transport movement of cell or subcellular component cell adhesion signal transduction multicellular organism development cell proliferation regulation of cell shape positive regulation of platelet activation folic acid transport single organismal cell-cell adhesion lung development positive regulation of cell migration tube morphogenesis lung alveolus development positive regulation of cellular component movement visceral serous pericardium development regulation of G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), Biotin, eBioscience™

Podoplanin Antibody (13-5381-82) in Flow

Product Details

13-5381-82

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!