Invitrogen

Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), PE-Cyanine7, eBioscience™

9 Published Figures

15 References

View all (70) Podoplanin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

Podoplanin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

Podoplanin Antibody (25-5381-82) in Flow

Staining of the TE-71 cell line with 0.06 µg of Golden Syrian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4914-82) (blue histogram) or 0.06 µg of Anti-Mouse Podoplanin PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor 450® (Product # 65-0863-14), were used for analysis.

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Product Details

25-5381-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio8.1.1 (8.1.1)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573460

Product Specific Information

Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.

Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity.

Nature immunology

Prados A,Onder L,Cheng HW,Mörbe U,Lütge M,Gil-Cruz C,Perez-Shibayama C,Koliaraki V,Ludewig B,Kollias G

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Sialoglycans on lymphatic endothelial cells augment interactions with Siglec-1 (CD169) of lymph node macrophages.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

D'Addio M,Frey J,Tacconi C,Commerford CD,Halin C,Detmar M,Cummings RD,Otto VI

25-5381-82 was used in Flow Cytometry to explore the role of sialoglycans on lymphatic endothelial cells in interactions with macrophage-expressed Siglec-1.

Mon Nov 01 00:00:00 EDT 2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Journal of cell science

de Winde CM,Makris S,Millward LJ,Cantoral-Rebordinos JA,Benjamin AC,Martínez VG,Acton SE

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

Lymph Node-Targeted Synthetically Glycosylated Antigen Leads to Antigen-Specific Immunological Tolerance.

Frontiers in immunology

Maulloo CD,Cao S,Watkins EA,Raczy MM,Solanki AS,Nguyen M,Reda JW,Shim HN,Wilson DS,Swartz MA,Hubbell JA

25-5381-82 was used in Flow cytometry/Cell sorting to demonstrate that targeting glycoconjugates to lymph node APCs under homeostatic conditions leads to increased accumulation in the lymph nodes.

Wed Dec 22 00:00:00 EST 2021

Sex-specific adipose tissue imprinting of regulatory T cells.

Nature

Vasanthakumar A,Chisanga D,Blume J,Gloury R,Britt K,Henstridge DC,Zhan Y,Torres SV,Liene S,Collins N,Cao E,Sidwell T,Li C,Spallanzani RG,Liao Y,Beavis PA,Gebhardt T,Trevaskis N,Nutt SL,Zajac JD,Davey RA,Febbraio MA,Mathis D,Shi W,Kallies A

25-5381-82 was used in Flow Cytometry to find that sex-specific differences in Treg cells from visceral adipose tissue are determined by the tissue niche in a sex-hormone-dependent manner to limit adipose tissue inflammation.

Sun Mar 01 00:00:00 EST 2020

Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.

PLoS biology

Fujimoto N,He Y,D'Addio M,Tacconi C,Detmar M,Dieterich LC

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Virus infection is controlled by hematopoietic and stromal cell sensing of murine cytomegalovirus through STING.

eLife

Piersma SJ,Poursine-Laurent J,Yang L,Barber GN,Parikh BA,Yokoyama WM

25-5381-82 was used in Flow cytometry/Cell sorting to contrast anti-viral effects of MyD88 versus STING in distinct cell types that are infected with murine CMV (MCMV).

Wed Jul 29 00:00:00 EDT 2020

Compartmentalized gut lymph node drainage dictates adaptive immune responses.

Nature

Esterházy D,Canesso MCC,Mesin L,Muller PA,de Castro TBR,Lockhart A,ElJalby M,Faria AMC,Mucida D

25-5381-82 was used in Flow cytometry/Cell sorting to show drainage of gut-draining lymph nodes can resolve conflicting tolerogenic and inflammatory intestinal responses by encouraging antigen targeting to specific gut segments.

Wed May 01 00:00:00 EDT 2019

Transcriptional profiling of breast cancer-associated lymphatic vessels reveals VCAM-1 as regulator of lymphatic invasion and permeability.

International journal of cancer

Dieterich LC,Kapaklikaya K,Cetintas T,Proulx ST,Commerford CD,Ikenberg K,Bachmann SB,Scholl J,Detmar M

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Fri Nov 15 00:00:00 EST 2019

Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury.

JCI insight

Riemondy KA,Jansing NL,Jiang P,Redente EF,Gillen AE,Fu R,Miller AJ,Spence JR,Gerber AN,Hesselberth JR,Zemans RL

25-5381 was used in Flow cytometry/Cell sorting to provide insight into the molecular mechanisms regulating alveolar regeneration and the pathogenesis of diseases resulting from a failure of regeneration.

Tue Mar 26 00:00:00 EDT 2019

Podoplanin is an important stromal prognostic marker in perihilar cholangiocarcinoma.

Oncology letters

Obulkasim H,Shi X,Wang J,Li J,Dai B,Wu P,Wang S,Wang X,Ding Y

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node.

Immunity

Baratin M,Simon L,Jorquera A,Ghigo C,Dembele D,Nowak J,Gentek R,Wienert S,Klauschen F,Malissen B,Dalod M,Bajénoff M

25-5381 was used in Immunohistochemistry-immunofluorescence to characterise a population of CX3CR1+ MERTK+ cells located in the T cell zone of lymph nodes.

Tue Aug 15 00:00:00 EDT 2017

Cornea lymphatics drive the CD8⁺ T-cell response to herpes simplex virus-1.

Immunology and cell biology

Gurung HR,Carr MM,Carr DJ

Published figure using Podoplanin monoclonal antibody (Product # 25-5381-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Thu Dec 01 00:00:00 EST 2016

Bioinformatics

Protein Aliases: aggrus; CTA-520D8.1; E11; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; Transmembrane glycoprotein E11

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Gene Aliases: Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha

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UniProt ID: (Mouse) Q62011

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Entrez Gene ID: (Mouse) 14726

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Function(s)

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity

Process(es)

cell morphogenesis lymphangiogenesis prostaglandin metabolic process water transport amino acid transport movement of cell or subcellular component cell adhesion signal transduction multicellular organism development cell proliferation regulation of cell shape positive regulation of platelet activation folic acid transport single organismal cell-cell adhesion lung development positive regulation of cell migration tube morphogenesis lung alveolus development positive regulation of cellular component movement visceral serous pericardium development regulation of G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), PE-Cyanine7, eBioscience™

Podoplanin Antibody (25-5381-82) in Flow

Product Details

25-5381-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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