Invitrogen

Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eFluor™ 660, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

27 Published Figures

32 References

View all (70) Podoplanin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 37

Podoplanin Antibody (50-5381-82) in IHC (P)

Immunohistochemical analysis of Podoplanin was performed using formalin-fixed paraffin-embedded mouse lung and mouse skeletal muscle tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degre... View More

Podoplanin Antibody in Western Blot (WB)

Podoplanin Antibody in Flow Cytometry (Flow)

Podoplanin Antibody in Immunohistochemistry (IHC)

Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed), Flow Cytometry (ICC/IF, IHC (PFA), Flow)

Podoplanin Antibody in Immunocytochemistry (ICC/IF)

Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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Product Details

50-5381-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

2 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.125 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Mouse, Rat

Host/Isotype

Syrian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBio8.1.1 (8.1.1)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151516

Product Specific Information

Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.

Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts.

The Journal of experimental medicine

Kerdidani D,Aerakis E,Verrou KM,Angelidis I,Douka K,Maniou MA,Stamoulis P,Goudevenou K,Prados A,Tzaferis C,Ntafis V,Vamvakaris I,Kaniaris E,Vachlas K,Sepsas E,Koutsopoulos A,Potaris K,Tsoumakidou M

50-5381-82 was used in Immunohistochemistry to characterize in the lungs a subset of antigen-presenting fibroblasts with tumor-suppressive properties and propose that cancer immunotherapies might be strongly dependent on in situ MHCII antigen presentation.

Mon Feb 07 00:00:00 EST 2022

Dysregulation of interaction between LOX^high fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Theranostics

Chen Y,Zhang T,Yao F,Gao X,Li D,Fu S,Mao L,Liu F,Zhang X,Xu Y,Deng J,Li W,Fan G,Xiao C,Chen Y,Wang L,Guo W,Zhou B

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunohistochemistry

Thu Mar 10 00:00:00 EST 2022

Vascularized lymph node transplantation successfully reverses lymphedema and maintains immunity in a rat lymphedema model.

Bioengineering & translational medicine

Sakarya AH,Huang CW,Yang CY,Hsiao HY,Chang FC,Huang JJ

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunohistochemistry

Thu Sep 01 00:00:00 EDT 2022

Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity.

Nature immunology

Prados A,Onder L,Cheng HW,Mörbe U,Lütge M,Gil-Cruz C,Perez-Shibayama C,Koliaraki V,Ludewig B,Kollias G

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Journal of cell science

de Winde CM,Makris S,Millward LJ,Cantoral-Rebordinos JA,Benjamin AC,Martínez VG,Acton SE

50-5381-82 was used in Flow Cytometry to show a multi-faceted FRC response to DCs, which requires CD44 and CD9 in addition to podoplanin.

Thu Jul 15 00:00:00 EDT 2021

Fibroblastic reticular cell response to dendritic cells requires coordinated activity of podoplanin, CD44 and CD9.

Journal of cell science

de Winde CM,Makris S,Millward LJ,Cantoral-Rebordinos JA,Benjamin AC,Martínez VG,Acton SE

50-5381-82 was used in Flow Cytometry to show a multi-faceted FRC response to DCs, which requires CD44 and CD9 in addition to podoplanin.

Thu Jul 15 00:00:00 EDT 2021

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

CLEC-2-dependent platelet subendothelial accumulation by flow disturbance contributes to atherogenesis in mice.

Theranostics

Tang C,Wang L,Sheng Y,Zheng Z,Xie Z,Wu F,You T,Ren L,Xia L,Ruan C,Zhu L

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Mon Feb 07 00:00:00 EST 2022

Podoplanin drives dedifferentiation and amoeboid invasion of melanoma.

iScience

de Winde CM,George SL,Crosas-Molist E,Hari-Gupta Y,Arp AB,Benjamin AC,Millward LJ,Makris S,Carver A,Imperatore V,Martínez VG,Sanz-Moreno V,Acton SE

50-5381-82 was used in Flow cytometry/Cell sorting to define podoplanin as a functional biomarker for dedifferentiated invasive melanoma and a promising migrastatic therapeutic target.

Fri Sep 24 00:00:00 EDT 2021

Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.

PLoS biology

Fujimoto N,He Y,D'Addio M,Tacconi C,Detmar M,Dieterich LC

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growth.

eLife

Thacker VV,Dhar N,Sharma K,Barrile R,Karalis K,McKinney JD

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunocytochemistry

Tue Nov 24 00:00:00 EST 2020

Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury.

JCI insight

Riemondy KA,Jansing NL,Jiang P,Redente EF,Gillen AE,Fu R,Miller AJ,Spence JR,Gerber AN,Hesselberth JR,Zemans RL

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunofluorescence

Tue Mar 26 00:00:00 EDT 2019

Transcriptional profiling of breast cancer-associated lymphatic vessels reveals VCAM-1 as regulator of lymphatic invasion and permeability.

International journal of cancer

Dieterich LC,Kapaklikaya K,Cetintas T,Proulx ST,Commerford CD,Ikenberg K,Bachmann SB,Scholl J,Detmar M

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Fri Nov 15 00:00:00 EST 2019

IL-4Rα-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells.

Cell reports

Dubey LK,Ludewig B,Luther SA,Harris NL

50-5381 was used in Flow cytometry/Cell sorting to determine the source of CXCL3 responsible for attracting CXCR5+ dendritic cells.

Tue May 21 00:00:00 EDT 2019

Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor.

Nature

Zacharias WJ,Frank DB,Zepp JA,Morley MP,Alkhaleel FA,Kong J,Zhou S,Cantu E,Morrisey EE

50-5381 was used in Immunohistochemistry to identify the AEP lineage as an evolutionarily conserved alveolar progenitor that represents a new target for human lung regeneration strategies.

Thu Mar 08 00:00:00 EST 2018

Macrophages impede CD8 T cells from reaching tumor cells and limit the efficacy of anti-PD-1 treatment.

Proceedings of the National Academy of Sciences of the United States of America

Peranzoni E,Lemoine J,Vimeux L,Feuillet V,Barrin S,Kantari-Mimoun C,Bercovici N,Guérin M,Biton J,Ouakrim H,Régnier F,Lupo A,Alifano M,Damotte D,Donnadieu E

50-5381 was used in Immunohistochemistry-immunofluorescence to show that exclusion of CD8 T cells from tumour islets is correlated with a poor clinical outcome and low lymphocyte motility in human lung squamous-cell carcinomas.

Tue Apr 24 00:00:00 EDT 2018

Podoplanin is an important stromal prognostic marker in perihilar cholangiocarcinoma.

Oncology letters

Obulkasim H,Shi X,Wang J,Li J,Dai B,Wu P,Wang S,Wang X,Ding Y

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.

Nature communications

Frye M,Taddei A,Dierkes C,Martinez-Corral I,Fielden M,Ortsäter H,Kazenwadel J,Calado DP,Ostergaard P,Salminen M,He L,Harvey NL,Kiefer F,Mäkinen T

50-5381 was used in Flow cytometry/Cell sorting to study whether extracellular matrix stiffness impacts lymphatic vascular development.

Tue Apr 17 00:00:00 EDT 2018

Cornea lymphatics drive the CD8⁺ T-cell response to herpes simplex virus-1.

Immunology and cell biology

Gurung HR,Carr MM,Carr DJ

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Stem cell function and stress response are controlled by protein synthesis.

Nature

Blanco S,Bandiera R,Popis M,Hussain S,Lombard P,Aleksic J,Sajini A,Tanna H,Cortés-Garrido R,Gkatza N,Dietmann S,Frye M

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunofluorescence

Thu Jun 16 00:00:00 EDT 2016

Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers.

Journal of hepatology

Lua I,Li Y,Zagory JA,Wang KS,French SW,Sévigny J,Asahina K

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunohistochemistry

Sun May 01 00:00:00 EDT 2016

Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers.

Journal of hepatology

Lua I,Li Y,Zagory JA,Wang KS,French SW,Sévigny J,Asahina K

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunohistochemistry

Sun May 01 00:00:00 EDT 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins.

Genesis (New York, N.Y. : 2000)

Ulvmar MH,Martinez-Corral I,Stanczuk L,Mäkinen T

50-5381 was used in Flow cytometry/Cell sorting to show that Pdgfrb-Cre does not target YS HemEC or YS-derived erythro-myeloid progenitors.

Wed Jun 01 00:00:00 EDT 2016

Nonvenous origin of dermal lymphatic vasculature.

Circulation research

Martinez-Corral I,Ulvmar MH,Stanczuk L,Tatin F,Kizhatil K,John SW,Alitalo K,Ortega S,Makinen T

50-5381 was used in Flow cytometry/Cell sorting to investigate the origin of murine lymphatic vasculature, showing a significant contribution from nonvenous cells.

Fri May 08 00:00:00 EDT 2015

Chronic lung injury by constitutive expression of activation-induced cytidine deaminase leads to focal mucous cell metaplasia and cancer.

PloS one

Kitamura J,Uemura M,Kurozumi M,Sonobe M,Manabe T,Hiai H,Date H,Kinoshita K

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunohistochemistry

Mon Mar 30 00:00:00 EDT 2015

Alveolar progenitor and stem cells in lung development, renewal and cancer.

Nature

Desai TJ,Brownfield DG,Krasnow MA

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunofluorescence

Thu Mar 13 00:00:00 EDT 2014

Dendritic cells control fibroblastic reticular network tension and lymph node expansion.

Nature

Acton SE,Farrugia AJ,Astarita JL,Mourão-Sá D,Jenkins RP,Nye E,Hooper S,van Blijswijk J,Rogers NC,Snelgrove KJ,Rosewell I,Moita LF,Stamp G,Turley SJ,Sahai E,Reis e Sousa C

50-5381 was used in Immunofluorescence to show that CLEC-2 modulation of PDPN signalling permits FRC network stretching and allows for the rapid lymph node expansion that is the critical hallmark of adaptive immunity.

Thu Oct 23 00:00:00 EDT 2014

Dendritic cells control fibroblastic reticular network tension and lymph node expansion.

Nature

Acton SE,Farrugia AJ,Astarita JL,Mourão-Sá D,Jenkins RP,Nye E,Hooper S,van Blijswijk J,Rogers NC,Snelgrove KJ,Rosewell I,Moita LF,Stamp G,Turley SJ,Sahai E,Reis e Sousa C

Thu Oct 23 00:00:00 EDT 2014

Mesodermal mesenchymal cells give rise to myofibroblasts, but not epithelial cells, in mouse liver injury.

Hepatology (Baltimore, Md.)

Lua I,James D,Wang J,Wang KS,Asahina K

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunofluorescence

Tue Jul 01 00:00:00 EDT 2014

C3 dysregulation due to factor H deficiency is mannan-binding lectin-associated serine proteases (MASP)-1 and MASP-3 independent in vivo.

Clinical and experimental immunology

Ruseva MM,Takahashi M,Fujita T,Pickering MC

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunofluorescence

Tue Apr 01 00:00:00 EDT 2014

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

Published figure using Podoplanin monoclonal antibody (Product # 50-5381-82) in Immunofluorescence

Sun Mar 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: aggrus; CTA-520D8.1; E11; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; Transmembrane glycoprotein E11

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Gene Aliases: Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha

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UniProt ID: (Mouse) Q62011

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Entrez Gene ID: (Mouse) 14726

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Function(s)

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity

Process(es)

cell morphogenesis lymphangiogenesis prostaglandin metabolic process water transport amino acid transport movement of cell or subcellular component cell adhesion signal transduction multicellular organism development cell proliferation regulation of cell shape positive regulation of platelet activation folic acid transport single organismal cell-cell adhesion lung development positive regulation of cell migration tube morphogenesis lung alveolus development positive regulation of cellular component movement visceral serous pericardium development regulation of G1/S transition of mitotic cell cycle

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eFluor™ 660, eBioscience™

Podoplanin Antibody (50-5381-82) in IHC (P)

Product Details

50-5381-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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