Invitrogen

CD4 Monoclonal Antibody (S3.5), Qdot™ 655

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CD4 Antibody (Q10007) in Flow

Qdot® 655 anti-human CD4 conjugate 655/20 band pass filter

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Product Details

Q10007

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 8 publications 8 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

S3.5

Immunogen

Human CD4

Conjugate

Qdot™ 655 Qdot™ 655 Qdot™ 655

Excitation/Emission Max

300/654 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11180600

Product Specific Information

QdotTM Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, QdotTM Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10015.

Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10007: peak excitation 405 (488) nm/peak emission 655 nm; recommended filter 655/20 nm.

Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

T cell phenotypes in women with Chlamydia trachomatis infection and influence of treatment on phenotype distributions.

Microbes and infection

Ogendi BMO,Bakshi RK,Gupta K,Kapil R,Brown LT,Jordan SJ,Sabbaj S,Press CG,Lee JY,Geisler WM

Q10007 was used in Flow cytometry/Cell sorting to evaluate differences in T cell phenotypes between Chlamydia trachomatis(CT)-infected women and CT-seronegative controls and investigate changes in T cell phenotype distributions after CT treatment and their association with reinfection.

Thu Mar 01 00:00:00 EST 2018

PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8⁺ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients.

Cancer cell

Naing A,Infante JR,Papadopoulos KP,Chan IH,Shen C,Ratti NP,Rojo B,Autio KA,Wong DJ,Patel MR,Ott PA,Falchook GS,Pant S,Hung A,Pekarek KL,Wu V,Adamow M,McCauley S,Mumm JB,Wong P,Van Vlasselaer P,Leveque J,Tannir NM,Oft M

Q10007 was used in Flow cytometry/Cell sorting to report that pegilodecakin induces hallmarks of CD8+ T cell immunity in cancer patients, including elevation of interferon-γ and GranzymeB, expansion and activation of intratumoral CD8+ T cells, and proliferation and expansion of LAG-3+ PD-1+ CD8+ T cells.

Mon Nov 12 00:00:00 EST 2018

Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women.

American journal of reproductive immunology (New York, N.Y. : 1989)

Ogendi BMO,Bakshi RK,Sabbaj S,Brown L,Lee JY,Kapil R,Geisler WM

Q10007 was used in Flow cytometry/Cell sorting to characterise the distinct peripheral and mucosal T-cell phenotypes in chlamydia-infected women.

Fri Dec 01 00:00:00 EST 2017

Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.

Immunological investigations

Kobie JJ,Treanor JJ,Ritchlin CT

Q10007 was used in flow cytometry to examine various DC after flu vaccination

Thu Apr 16 00:00:00 EDT 2015

Dual role of novel ingenol derivatives from Euphorbia tirucalli in HIV replication: inhibition of de novo infection and activation of viral LTR.

PloS one

Abreu CM,Price SL,Shirk EN,Cunha RD,Pianowski LF,Clements JE,Tanuri A,Gama L

Q10007 was used in Flow cytometry/Cell sorting to study the use of three ingenol synthetic derivatives to activate the HIV-LTR in latently infected cells whilst inhibiting replication.

Mon Jan 05 00:00:00 EST 2015

Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.

Clinical immunology (Orlando, Fla.)

McArthur MA,Sztein MB

Q10007 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which Staphylococcal enterotoxin B and other superantigens cause toxic shock syndrome.

Fri Feb 01 00:00:00 EST 2013

Leukocyte composition of human breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM

Q10007 was used in flow cytometry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery

Tue Feb 21 00:00:00 EST 2012

Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization.

PloS one

McArthur MA,Sztein MB

Q10007 was used in flow cytometry to explore the multifunctional IL-17A responses against S. Typhi antigens in T memory subsets.

Tue Oct 02 00:00:00 EDT 2012

Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

Berthoud TK,Hamill M,Lillie PJ,Hwenda L,Collins KA,Ewer KJ,Milicic A,Poyntz HC,Lambe T,Fletcher HA,Hill AV,Gilbert SC

Q10007 was used in flow cytometry to discuss the use of internal antigens to provide better protection against multiple influenza subtypes.

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (S3.5), Qdot™ 655

CD4 Antibody (Q10007) in Flow

Product Details

Q10007

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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