Invitrogen

CD4 Monoclonal Antibody (S3.5), Qdot™ 605

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CD4 Antibody (Q10008) in Flow

Qdot® 605 anti-human CD4 conjugate 605/20 band pass filter

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Product Details

Q10008

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 24 publications 24 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

S3.5

Immunogen

Human CD4

Conjugate

Qdot™ 605 Qdot™ 605 Qdot™ 605

Excitation/Emission Max

300/603 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11180611

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

HIV silencing and cell survival signatures in infected T cell reservoirs.

Nature

Clark IC,Mudvari P,Thaploo S,Smith S,Abu-Laban M,Hamouda M,Theberge M,Shah S,Ko SH,Pérez L,Bunis DG,Lee JS,Kilam D,Zakaria S,Choi S,Darko S,Henry AR,Wheeler MA,Hoh R,Butrus S,Deeks SG,Quintana FJ,Douek DC,Abate AR,Boritz EA

Q10008 was used in Flow Cytometry to reveal that HIV-infected memory CD4 T cells under antiretroviral therapy are a distinctive population with host gene expression patterns that favour HIV silencing, cell survival and cell proliferation, with important implications for the development of HIV cure strategies.

Wed Feb 01 00:00:00 EST 2023

HIV silencing and cell survival signatures in infected T cell reservoirs.

Nature

Wed Feb 01 00:00:00 EST 2023

A high OXPHOS CD8 T cell subset is predictive of immunotherapy resistance in melanoma patients.

The Journal of experimental medicine

Li C,Phoon YP,Karlinsey K,Tian YF,Thapaliya S,Thongkum A,Qu L,Matz AJ,Cameron M,Cameron C,Menoret A,Funchain P,Song JM,Diaz-Montero CM,Tamilselvan B,Golden JB,Cartwright M,Rodriguez A,Bonin C,Vella A,Zhou B,Gastman BR

Q10008 was used in Flow cytometry/Cell sorting to conclude that CD8+ TOXPHOS cells represent a critical immune population to assess ICI response with the potential to be a new target to improve outcomes in melanoma patients.

Mon Jan 03 00:00:00 EST 2022

CARD8 inflammasome activation triggers pyroptosis in human T cells.

The EMBO journal

Linder A,Bauernfried S,Cheng Y,Albanese M,Jung C,Keppler OT,Hornung V

Published figure using CD4 monoclonal antibody (Product # Q10008) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CARD8 inflammasome activation triggers pyroptosis in human T cells.

The EMBO journal

Linder A,Bauernfried S,Cheng Y,Albanese M,Jung C,Keppler OT,Hornung V

Published figure using CD4 monoclonal antibody (Product # Q10008) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Differentiation into an Effector Memory Phenotype Potentiates HIV-1 Latency Reversal in CD4⁺ T Cells.

Journal of virology

Kulpa DA,Talla A,Brehm JH,Ribeiro SP,Yuan S,Bebin-Blackwell AG,Miller M,Barnard R,Deeks SG,Hazuda D,Chomont N,Sékaly RP

Q10008 was used in Flow Cytometry to support the idea that the effector memory phenotype supports HIV-1 latency reversal in CD4+ T cells.

Sun Dec 15 00:00:00 EST 2019

PD-1 blockade potentiates HIV latency reversal ex vivo in CD4⁺ T cells from ART-suppressed individuals.

Nature communications

Fromentin R,DaFonseca S,Costiniuk CT,El-Far M,Procopio FA,Hecht FM,Hoh R,Deeks SG,Hazuda DJ,Lewin SR,Routy JP,Sékaly RP,Chomont N

Q10008 was used in Flow cytometry/Cell sorting to investigate whether PD-1 plays a functional role in HIV latency and reservoir persistence.

Mon Feb 18 00:00:00 EST 2019

Cycling CD4+ T cells in HIV-infected immune nonresponders have mitochondrial dysfunction.

The Journal of clinical investigation

Younes SA,Talla A,Pereira Ribeiro S,Saidakova EV,Korolevskaya LB,Shmagel KV,Shive CL,Freeman ML,Panigrahi S,Zweig S,Balderas R,Margolis L,Douek DC,Anthony DD,Pandiyan P,Cameron M,Sieg SF,Calabrese LH,Rodriguez B,Lederman MM

Q10008 was used in Flow cytometry/Cell sorting to demonstrate that cells with the phenotype and transcriptional profile of regulatory T cells are enriched among cycling cells in health and in HIV infection.

Thu Nov 01 00:00:00 EDT 2018

Early-onset childhood atopic dermatitis is related to NLRP2 repression.

The Journal of allergy and clinical immunology

Thürmann L,Grützmann K,Klös M,Bieg M,Winter M,Polte T,Bauer T,Schick M,Bewerunge-Hudler M,Röder S,Bauer M,Wissenbach DK,Sack U,Weichenhan D,Mücke O,Plass C,Borte M,von Bergen M,Lehmann I,Eils R,Trump S

Q10008 was used in Flow cytometry/Cell sorting to help elucidate the relevance of NLRPs in childhood AD.

Sun Apr 01 00:00:00 EDT 2018

PD-1 Expression on Mycobacterium tuberculosis-Specific CD4 T Cells Is Associated With Bacterial Load in Human Tuberculosis.

Frontiers in immunology

Day CL,Abrahams DA,Bunjun R,Stone L,de Kock M,Walzl G,Wilkinson RJ,Burgers WA,Hanekom WA

Q10008 was used in Flow Cytometry to study the effect of PD-1 expression on bacterial load and functional capacity of Mycobacterial tuberculosis specific T cell responses.

Mon Sep 30 00:00:00 EDT 2019

Novel mechanisms to inhibit HIV reservoir seeding using Jak inhibitors.

PLoS pathogens

Gavegnano C,Brehm JH,Dupuy FP,Talla A,Ribeiro SP,Kulpa DA,Cameron C,Santos S,Hurwitz SJ,Marconi VC,Routy JP,Sabbagh L,Schinazi RF,Sékaly RP

Q10008 was used in Flow cytometry/Cell sorting to evaluate the ability of tofacitinib and ruxolitinib, two FDA-approved Jak inhibitors, to block seeding and maintenance of the HIV reservoir in vitro.

Fri Dec 01 00:00:00 EST 2017

HIV-1 Infection Is Associated with Depletion and Functional Impairment of Mycobacterium tuberculosis-Specific CD4 T Cells in Individuals with Latent Tuberculosis Infection.

Journal of immunology (Baltimore, Md. : 1950)

Day CL,Abrahams DA,Harris LD,van Rooyen M,Stone L,de Kock M,Hanekom WA

Q10008 was used in Flow cytometry/Cell sorting to provide new insights into the parameters of M. tuberculosis-specific CD4 T cell immunity that are impaired in HIV-infected individuals with LTBI, which may contribute to their increased risk of developing active tuberculosis disease.

Fri Sep 15 00:00:00 EDT 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Q10008 was used in Flow cytometry/Cell sorting to propose that universally high mycobacterial sensitization in persons from high TB burden settings may impart broad inhibition of mycobacterial growth.

Wed Oct 18 00:00:00 EDT 2017

Nonprogressing HIV-infected children share fundamental immunological features of nonpathogenic SIV infection.

Science translational medicine

Muenchhoff M,Adland E,Karimanzira O,Crowther C,Pace M,Csala A,Leitman E,Moonsamy A,McGregor C,Hurst J,Groll A,Mori M,Sinmyee S,Thobakgale C,Tudor-Williams G,Prendergast AJ,Kloverpris H,Roider J,Leslie A,Shingadia D,Brits T,Daniels S,Frater J,Willberg CB,Walker BD,Ndung'u T,Jooste P,Moore PL,Morris L,Goulder P

Q10008 was used in Flow cytometry/Cell sorting to investigate pediatric infection, where AIDS typically develops more rapidly than in adults.

Wed Sep 28 00:00:00 EDT 2016

CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART.

PLoS pathogens

Fromentin R,Bakeman W,Lawani MB,Khoury G,Hartogensis W,DaFonseca S,Killian M,Epling L,Hoh R,Sinclair E,Hecht FM,Bacchetti P,Deeks SG,Lewin SR,Sékaly RP,Chomont N

Q10008 was used in flow cytometry test if CD4 positive T cells expressing immune checkpoint molecules are enriched in HIV-infected cells in patients receiving antiretroviral therapy

Fri Jul 01 00:00:00 EDT 2016

Analysis of Intracellular Ca(2+) Mobilization in Human NK Cell Subsets by Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)

Theorell J,Bryceson YT

Q10008 was used in flow cytometry to utilize flow cytometry to analyze intracellular Ca(2+) mobilization in human NK cell subsets

Mon Dec 25 00:00:00 EST 2017

Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry.

Journal of immunological methods

Kagina BM,Mansoor N,Kpamegan EP,Penn-Nicholson A,Nemes E,Smit E,Gelderbloem S,Soares AP,Abel B,Keyser A,Sidibana M,Hughes JE,Kaplan G,Hussey GD,Hanekom WA,Scriba TJ

Q10008 was used in flow cytometry to assess the effects of long-term cryopreservation of fixed cells on the measurement of mycobacteria induced CD4 T cell immunity

Sun Feb 01 00:00:00 EST 2015

Antiviral Innate Immune Activation in HIV-Infected Adults Negatively Affects H1/IC31-Induced Vaccine-Specific Memory CD4+ T Cells.

Clinical and vaccine immunology : CVI

Lenz N,Schindler T,Kagina BM,Zhang JD,Lukindo T,Mpina M,Bang P,Kromann I,Hoff ST,Andersen P,Reither K,Churchyard GJ,Certa U,Daubenberger CA

Q10008 was used in flow cytometry to study the immune responses of HIV-infected volunteers vaccinated against tuberculosis.

Wed Jul 01 00:00:00 EDT 2015

Distinct T-cell responses when BCG vaccination is delayed from birth to 6 weeks of age in Ugandan infants.

The Journal of infectious diseases

Lutwama F,Kagina BM,Wajja A,Waiswa F,Mansoor N,Kirimunda S,Hughes EJ,Kiwanuka N,Joloba ML,Musoke P,Scriba TJ,Mayanja-Kizza H,Day CL,Hanekom WA

Q10008 was used in flow cytometry to assess the effect of delayed administration of the BCG vaccine on the induced immune response in Ugandan children

Sat Mar 01 00:00:00 EST 2014

Hypomethylation at the regulatory T cell-specific demethylated region in CD25hi T cells is decoupled from FOXP3 expression at the inflamed site in childhood arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Bending D,Pesenacker AM,Ursu S,Wu Q,Lom H,Thirugnanabalan B,Wedderburn LR

Q10008 was used in Flow cytometry/Cell sorting to study dissociation of CD25 and FOXP3 in CD4+ T cells at the site of inflammation in patients with juvenile arthritis..

Mon Sep 15 00:00:00 EDT 2014

The novel tuberculosis vaccine, AERAS-402, is safe in healthy infants previously vaccinated with BCG, and induces dose-dependent CD4 and CD8T cell responses.

Vaccine

Kagina BM,Tameris MD,Geldenhuys H,Hatherill M,Abel B,Hussey GD,Scriba TJ,Mahomed H,Sadoff JC,Hanekom WA,Mansoor N,Hughes J,de Kock M,Whatney W,Africa H,Krohn C,Veldsman A,Kany AL,Douoguih M,Pau MG,Hendriks J,McClainc B,Benko J,Snowden MA,Hokey DA

Q10008 was used in flow cytometry to test the safety and immunogenicity of an adenovirus 35-vectored tuberculosis vaccine candidate AERAS-402 in infants

Tue Oct 14 00:00:00 EDT 2014

Overweight and obese adult humans have a defective cellular immune response to pandemic H1N1 influenza A virus.

Obesity (Silver Spring, Md.)

Paich HA,Sheridan PA,Handy J,Karlsson EA,Schultz-Cherry S,Hudgens MG,Noah TL,Weir SS,Beck MA

Q10008 was used in flow cytometry to study defective cellular immune response to pandemic H1N1 influenza A virus in obese and overweight adult humans

Fri Nov 01 00:00:00 EDT 2013

Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo.

PLoS pathogens

Introini A,Vanpouille C,Lisco A,Grivel JC,Margolis L

Q10008 was used in flow cytometry to study facilitation of HIV-1 transmission to cervico-vaginal tissue ex vivo by interleukin-7

Fri Feb 01 00:00:00 EST 2013

Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.

European journal of immunology

Dintwe OB,Day CL,Smit E,Nemes E,Gray C,Tameris M,McShane H,Mahomed H,Hanekom WA,Scriba TJ

Q10008 was used in flow cytometry to characterize T cells after MVA85A vaccination

Sun Sep 01 00:00:00 EDT 2013

Quantitative and qualitative differences in the T cell response to HIV in uninfected Ugandans exposed or unexposed to HIV-infected partners.

Journal of virology

Pala P,Serwanga J,Watera C,Ritchie AJ,Moodie Z,Wang M,Goonetilleke N,Birabwa E,Hughes P,Senkaali D,Nakiboneka R,Grosskurth H,Haynes B,McMichael A,Kaleebu P

Q10008 was used in flow cytometry to characterize T cell responses of HIV-exposed, yet persistently uninfected individuals.

Thu Aug 01 00:00:00 EDT 2013

Differential regulatory T cell activity in HIV type 1-exposed seronegative individuals.

AIDS research and human retroviruses

Pattacini L,Murnane PM,Kahle EM,Bolton MJ,Delrow JJ,Lingappa JR,Katabira E,Donnell D,McElrath MJ,Baeten JM,Lund JM

Q10008 was used in flow cytometry to determine the role of conventional and regulatory T cells in protection from HIV-1 infection.

Tue Oct 01 00:00:00 EDT 2013

The Thai phase III trial (RV144) vaccine regimen induces T cell responses that preferentially target epitopes within the V2 region of HIV-1 envelope.

Journal of immunology (Baltimore, Md. : 1950)

de Souza MS,Ratto-Kim S,Chuenarom W,Schuetz A,Chantakulkij S,Nuntapinit B,Valencia-Micolta A,Thelian D,Nitayaphan S,Pitisuttithum P,Paris RM,Kaewkungwal J,Michael NL,Rerks-Ngarm S,Mathieson B,Marovich M,Currier JR,Kim JH

Q10008 was used in flow cytometry to assess cell-mediated immune responses in Thai HIV phase III prime/boost vaccine trial patients

Tue May 15 00:00:00 EDT 2012

Functional capacity of Mycobacterium tuberculosis-specific T cell responses in humans is associated with mycobacterial load.

Journal of immunology (Baltimore, Md. : 1950)

Day CL,Abrahams DA,Lerumo L,Janse van Rensburg E,Stone L,O'rie T,Pienaar B,de Kock M,Kaplan G,Mahomed H,Dheda K,Hanekom WA

Q10008 was used in flow cytometry to examine the relationship between T cells and antigen load in patients with chronic Mycobacterium tuberculosis infection.

Thu Sep 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

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CD4 Monoclonal Antibody (S3.5), Qdot™ 605

CD4 Antibody (Q10008) in Flow

Product Details

Q10008

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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