Invitrogen

RANGAP1 Monoclonal Antibody (19C7)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

16 Published Figures

23 References

View all (21) RANGAP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

RANGAP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

RANGAP1 Antibody (33-0800) in ICC/IF

Immunofluorescence analysis of RanGAP-1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RanGAP-1 Mouse Monoclonal Antibody (Product # 33-0800) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a d... View More

RANGAP1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

RANGAP1 Antibody in Immunohistochemistry (IHC)

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Product Details

33-0800

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 8 publications 8 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

19C7

Immunogen

A fusion protein consisting of the first 203 amino-terminal residues of the mouse RanGAP-1 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533099

Target Information

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrative genetic analysis illuminates ALS heritability and identifies risk genes.

Nature communications

Megat S,Mora N,Sanogo J,Roman O,Catanese A,Alami NO,Freischmidt A,Mingaj X,De Calbiac H,Muratet F,Dirrig-Grosch S,Dieterle S,Van Bakel N,Müller K,Sieverding K,Weishaupt J,Andersen PM,Weber M,Neuwirth C,Margelisch M,Sommacal A,Van Eijk KR,Veldink JH,Lautrette G,Couratier P,Camuzat A,Le Ber I,Grassano M,Chio A,Boeckers T,Ludolph AC,Roselli F,Yilmazer-Hanke D,Millecamps S,Kabashi E,Storkebaum E,Sellier C,Dupuis L

33-0800 was used in Immunocytochemistry and Western Blottig to show that ALS heritability is enriched in splicing variants and in binding sites of 6 RNA-binding proteins including TDP-43 and FUS.

Fri Jan 20 00:00:00 EST 2023

Integrative genetic analysis illuminates ALS heritability and identifies risk genes.

Nature communications

33-0800 was used in Immunocytochemistry and Western Blottig to show that ALS heritability is enriched in splicing variants and in binding sites of 6 RNA-binding proteins including TDP-43 and FUS.

Fri Jan 20 00:00:00 EST 2023

MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus.

Journal of virology

Sloan E,Orr A,Everett RD

33-0800 was used in Western Blotting to reveal that MORC3 is an antiviral factor which plays an important role during HSV-1 and HCMV infection.

Sat Oct 01 00:00:00 EDT 2016

Analysis of the SUMO2 Proteome during HSV-1 Infection.

PLoS pathogens

Sloan E,Tatham MH,Groslambert M,Glass M,Orr A,Hay RT,Everett RD

33-0800 was used in western blot to summarize HSV-1 infection and the SUMO2 proteome

Wed Jul 01 00:00:00 EDT 2015

The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function.

Molecular biology of the cell

Chow KH,Elgort S,Dasso M,Powers MA,Ullman KS

33-0800 was used in immunocytochemistry and western blot to identify a new role for the pore-associated SENPs in nucleoporin homeostasis and proper configuration of the nuclear pore complex.

Wed Jan 01 00:00:00 EST 2014

The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function.

Molecular biology of the cell

Chow KH,Elgort S,Dasso M,Powers MA,Ullman KS

33-0800 was used in immunocytochemistry and western blot to identify a new role for the pore-associated SENPs in nucleoporin homeostasis and proper configuration of the nuclear pore complex.

Wed Jan 01 00:00:00 EST 2014

A pathway linking oxidative stress and the Ran GTPase system in progeria.

Molecular biology of the cell

Datta S,Snow CJ,Paschal BM

Tue Apr 01 00:00:00 EDT 2014

Ubiquitylation-dependent localization of PLK1 in mitosis.

Nature cell biology

Beck J,Maerki S,Posch M,Metzger T,Persaud A,Scheel H,Hofmann K,Rotin D,Pedrioli P,Swedlow JR,Peter M,Sumara I

33-0800 was used in Functional Assay to suggest that CUL3-KLHL22-mediated ubiquitylation signals degradation-independent removal of PLK1 from kinetochores and SAC satisfaction, which are required for faithful mitosis.

Mon Apr 01 00:00:00 EDT 2013

Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe.

Cell death & disease

Hashizume C,Kobayashi A,Wong RW

33-0800 was used in immunocytochemistry and western blot to study the role of nucleoporin RanBP2/Nup358 in chromosomal alignment and mitotic integrity

Thu Oct 10 00:00:00 EDT 2013

Down-modulation of nucleoporin RanBP2/Nup358 impaired chromosomal alignment and induced mitotic catastrophe.

Cell death & disease

Hashizume C,Kobayashi A,Wong RW

33-0800 was used in immunocytochemistry and western blot to study the role of nucleoporin RanBP2/Nup358 in chromosomal alignment and mitotic integrity

Thu Oct 10 00:00:00 EDT 2013

The interaction between importin-α and Nup153 promotes importin-α/β-mediated nuclear import.

Traffic (Copenhagen, Denmark)

Ogawa Y,Miyamoto Y,Oka M,Yoneda Y

33-0800 was used in Immunocytochemistry-immunoflourescence to show that in conjunction with Nup153, importin α contributes to directionally biased exit of classical nuclear localisation signal-containing cargo to the nuclear side of nuclear pore complexes.

Sun Jul 01 00:00:00 EDT 2012

Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly.

Molecular biology of the cell

Funakoshi T,Clever M,Watanabe A,Imamoto N

33-0800 was used in immunocytochemistry to investigate the contribution of pore membrane protein-121 to nuclear pore assembly.

Fri Apr 01 00:00:00 EDT 2011

Detection of protein SUMOylation in vivo.

Nature protocols

Tatham MH,Rodriguez MS,Xirodimas DP,Hay RT

33-0800 was used in western blot to describe a method to detect SUMOylation

Thu Dec 03 00:00:00 EST 2009

The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle.

Molecular & cellular proteomics : MCP

Schimmel J,Larsen KM,Matic I,van Hagen M,Cox J,Mann M,Andersen JS,Vertegaal AC

33-0800 was used in western blot to investigate the cross-talk between sumoylation and ubiquitination

Sat Nov 01 00:00:00 EDT 2008

NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression.

Proceedings of the National Academy of Sciences of the United States of America

Rosendorff A,Sakakibara S,Lu S,Kieff E,Xuan Y,DiBacco A,Shi Y,Shi Y,Gill G

Published figure using RANGAP1 monoclonal antibody (Product # 33-0800) in Western Blot

Tue Apr 04 00:00:00 EDT 2006

Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics.

Molecular & cellular proteomics : MCP

Vertegaal AC,Andersen JS,Ogg SC,Hay RT,Mann M,Lamond AI

33-0800 was used in western blot to investigate the target protein preferences of SUMO-1 and SUMO-2

Fri Dec 01 00:00:00 EST 2006

Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development.

Molecular and cellular biology

Yamaguchi T,Sharma P,Athanasiou M,Kumar A,Yamada S,Kuehn MR

Wed Jun 01 00:00:00 EDT 2005

Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development.

Molecular and cellular biology

Yamaguchi T,Sharma P,Athanasiou M,Kumar A,Yamada S,Kuehn MR

Wed Jun 01 00:00:00 EDT 2005

A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers.

Molecular & cellular proteomics : MCP

Rosas-Acosta G,Russell WK,Deyrieux A,Russell DH,Wilson VG

Published figure using RANGAP1 monoclonal antibody (Product # 33-0800) in Western Blot

Sat Jan 01 00:00:00 EST 2005

SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.

The Journal of cell biology

Joseph J,Tan SH,Karpova TS,McNally JG,Dasso M

Mon Feb 18 00:00:00 EST 2002

Association of the human SUMO-1 protease SENP2 with the nuclear pore.