Invitrogen

SOX2 Monoclonal Antibody (Btjce), eFluor™ 570, eBioscience™

SOX2 Antibody in Immunocytochemistry (ICC/IF)

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SOX2 Antibody (41-9811-82) in ICC/IF

Immunocytochemistry of F9 cells using 5 µg/mL of Rat IgG2a Isotype Control eFluor® 570 (left) or 5 µg/mL of Anti-Human/Mouse Sox2 eFluor® 570 (right). Nuclei are stained with DAPI.

SOX2 Antibody in Immunohistochemistry (IHC)

SOX2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

SOX2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

SOX2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

41-9811-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Btjce

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573650

Product Specific Information

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).

Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.

Applications Reported: This Btjce antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This Btjce antibody has been tested by immunocytochemistry of fixed and permeabilized F9 cells. Thsi can be used at less than 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Bacterial expression of region after aa135 (homology between species but not to other family members)

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Thioredoxin facilitates hepatocellular carcinoma stemness and metastasis by increasing BACH1 stability to activate the AKT/mTOR pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang C,Zhang L,Cao M,Fu Z,Wang H,Zhang S,Zhu K,Hou Z,Cui J,Yue P,Guo H,Zhang T

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Western Blot

Thu Jun 01 00:00:00 EDT 2023

A gastruloid model of the interaction between embryonic and extra-embryonic cell types.

Journal of tissue engineering

Bérenger-Currias NM,Mircea M,Adegeest E,van den Berg PR,Feliksik M,Hochane M,Idema T,Tans SJ,Semrau S

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Sat Jul 16 00:00:00 EDT 2022

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis.

International journal of molecular sciences

Belgacemi R,Danopoulos S,Deutsch G,Glass I,Dormoy V,Bellusci S,Al Alam D

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Mon May 09 00:00:00 EDT 2022

DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential.

Developmental cell

Frederico B,Martins I,Chapela D,Gasparrini F,Chakravarty P,Ackels T,Piot C,Almeida B,Carvalho J,Ciccarelli A,Peddie CJ,Rogers N,Briscoe J,Guillemot F,Schaefer AT,Saúde L,Reis e Sousa C

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Mon Aug 22 00:00:00 EDT 2022

The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.

Acta neuropathologica communications

Lambert E,Saha O,Soares Landeira B,Melo de Farias AR,Hermant X,Carrier A,Pelletier A,Gadaut J,Davoine L,Dupont C,Amouyel P,Bonnefond A,Lafont F,Abdelfettah F,Verstreken P,Chapuis J,Barois N,Delahaye F,Dermaut B,Lambert JC,Costa MR,Dourlen P

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Sat Jan 08 00:00:00 EST 2022

Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.

Cell stem cell

Kinoshita M,Barber M,Mansfield W,Cui Y,Spindlow D,Stirparo GG,Dietmann S,Nichols J,Smith A

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Thu Mar 04 00:00:00 EST 2021

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

Wnt/β-catenin signalling is dispensable for adult neural stem cell homeostasis and activation.

Development (Cambridge, England)

Austin SHL,Gabarró-Solanas R,Rigo P,Paun O,Harris L,Guillemot F,Urbán N

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Fri Oct 15 00:00:00 EDT 2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Science advances

Mirzaei R,Gordon A,Zemp FJ,Kumar M,Sarkar S,Luchman HA,Bellail AC,Hao C,Mahoney DJ,Dunn JF,Bose P,Yong VW

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Fri Nov 05 00:00:00 EDT 2021

Induction of inverted morphology in brain organoids by vertical-mixing bioreactors.

Communications biology

Suong DNA,Imamura K,Inoue I,Kabai R,Sakamoto S,Okumura T,Kato Y,Kondo T,Yada Y,Klein WL,Watanabe A,Inoue H

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry (Frozen)

Fri Oct 22 00:00:00 EDT 2021

Modulating mesendoderm competence during human germ layer differentiation.

Cell reports

Valcourt JR,Huang RE,Kundu S,Venkatasubramanian D,Kingston RE,Ramanathan S

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Tue Nov 09 00:00:00 EST 2021

Elp2 mutations perturb the epitranscriptome and lead to a complex neurodevelopmental phenotype.

Nature communications

Kojic M,Gawda T,Gaik M,Begg A,Salerno-Kochan A,Kurniawan ND,Jones A,Drożdżyk K,Kościelniak A,Chramiec-Głąbik A,Hediyeh-Zadeh S,Kasherman M,Shim WJ,Sinniah E,Genovesi LA,Abrahamsen RK,Fenger CD,Madsen CG,Cohen JS,Fatemi A,Stark Z,Lunke S,Lee J,Hansen JK,Boxill MF,Keren B,Marey I,Saenz MS,Brown K,Alexander SA,Mureev S,Batzilla A,Davis MJ,Piper M,Bodén M,Burne THJ,Palpant NJ,Møller RS,Glatt S,Wainwright BJ

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Tue May 11 00:00:00 EDT 2021

Quantitative analysis of signaling responses during mouse primordial germ cell specification.

Biology open

Morgani SM,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Sat May 15 00:00:00 EDT 2021

Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.

Cells

Siljee S,Buchanan O,Brasch HD,Bockett N,Patel J,Paterson E,Purdie GL,Davis PF,Itinteang T,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2021

The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos.

eLife

Morgani SM,Su J,Nichols J,Massagué J,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry (Frozen)

Fri Apr 30 00:00:00 EDT 2021

Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging.

Frontiers in aging neuroscience

Sugiura A,Shimizu T,Kameyama T,Maruo T,Kedashiro S,Miyata M,Mizutani K,Takai Y

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Tue Mar 30 00:00:00 EDT 2021

Embryonic disc formation following post-hatching bovine embryo development in vitro.

Reproduction (Cambridge, England)

Ramos-Ibeas P,Lamas-Toranzo I,Martínez-Moro Á,de Frutos C,Quiroga AC,Zurita E,Bermejo-Álvarez P

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells.

Viruses

Trus I,Berube N,Jiang P,Rak J,Gerdts V,Karniychuk U

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunocytochemistry

Mon May 25 00:00:00 EDT 2020

Cancer Stem Cells in Head and Neck Metastatic Malignant Melanoma Express Components of the Renin-Angiotensin System.

Life (Basel, Switzerland)

Siljee S,Pilkington T,Brasch HD,Bockett N,Patel J,Paterson E,Davis PF,Tan ST

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry (Paraffin)

Mon Nov 02 00:00:00 EST 2020

Spatial-Temporal Lineage Restrictions of Embryonic p63⁺ Progenitors Establish Distinct Stem Cell Pools in Adult Airways.

Developmental cell

Yang Y,Riccio P,Schotsaert M,Mori M,Lu J,Lee DK,García-Sastre A,Xu J,Cardoso WV

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunohistochemistry

Mon Mar 26 00:00:00 EDT 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.

eLife

Morgani SM,Metzger JJ,Nichols J,Siggia ED,Hadjantonakis AK

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunofluorescence

Wed Feb 07 00:00:00 EST 2018

BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.

Nature communications

Lazarus KA,Hadi F,Zambon E,Bach K,Santolla MF,Watson JK,Correia LL,Das M,Ugur R,Pensa S,Becker L,Campos LS,Ladds G,Liu P,Evan GI,McCaughan FM,Le Quesne J,Lee JH,Calado D,Khaled WT

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunofluorescence

Mon Aug 20 00:00:00 EDT 2018

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using SOX2 monoclonal antibody (Product # 41-9811-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

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Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb

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UniProt ID: (Human) P48431, (Mouse) P48432

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Entrez Gene ID: (Human) 6657, (Mouse) 20674

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Function(s)

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding transcription factor binding chromatin DNA binding protein heterodimerization activity HMG box transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation eye development endodermal cell fate specification chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell cycle arrest response to wounding regulation of gene expression glial cell fate commitment pituitary gland development adenohypophysis development positive regulation of cell-cell adhesion forebrain development somatic stem cell population maintenance tissue regeneration regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter inner ear development negative regulation of epithelial cell proliferation response to growth factor negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance cell fate specification positive regulation of neuroblast proliferation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development sensory perception of sound response to organic substance stem cell population maintenance forebrain neuron differentiation cerebral cortex development negative regulation of Wnt signaling pathway male genitalia development positive regulation of epithelial cell differentiation olfactory placode formation response to retinoic acid inner ear morphogenesis tongue development cell fate commitment negative regulation of cell differentiation positive regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of Notch signaling pathway pigment biosynthetic process lung alveolus development embryonic organ development anatomical structure formation involved in morphogenesis neuron fate commitment diencephalon morphogenesis stem cell differentiation regulation of neurogenesis detection of mechanical stimulus involved in sensory perception of sound detection of mechanical stimulus involved in equilibrioception retina morphogenesis in camera-type eye lens induction in camera-type eye epithelial tube branching involved in lung morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOX2 Monoclonal Antibody (Btjce), eFluor™ 570, eBioscience™

SOX2 Antibody (41-9811-82) in ICC/IF

Product Details

41-9811-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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