Invitrogen

Snail1 Monoclonal Antibody (20C8), Alexa Fluor™ 488, eBioscience™

11 Published Figures

11 References

View all (36) SNAIL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

Snail1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Snail1 Antibody (53-9859-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized MCF7 cells using 20 µg/mL Mouse IgG2a Alexa Fluor® 488 Isotype Control (left) or 20 µg/mL Anti-Human Snail1 Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Snail1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

Product Details

53-9859-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

Immunocytochemistry (ICC/IF)

20 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-Dependent

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

20C8

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574483

Product Specific Information

Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.

Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, immunocytochemistry, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This 20C8 antibody has been tested by immunocytochemistry on fixed and permeabilized MCF7 cells and by immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma. This antibody can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer.

Cell death & disease

Guo F,Ma J,Li C,Liu S,Wu W,Li C,Wang J,Wang J,Li Z,Zhai J,Sun F,Zhou Y,Guo C,Qian H,Xu B

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Western Blot

Tue Apr 18 00:00:00 EDT 2023

Aberrant Akt2 signaling in the RPE may contribute to retinal fibrosis process in diabetic retinopathy.

Cell death discovery

Daley R,Maddipatla V,Ghosh S,Chowdhury O,Hose S,Zigler JS,Sinha D,Liu H

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Western Blot

Thu Jul 13 00:00:00 EDT 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunoprecipitation

Fri Feb 03 00:00:00 EST 2023

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Western Blot

Fri Dec 31 00:00:00 EST 2021

MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunocytochemistry

Thu Jul 15 00:00:00 EDT 2021

MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunocytochemistry

Thu Jul 15 00:00:00 EDT 2021

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunocytochemistry

Mon Aug 31 00:00:00 EDT 2020

HOXC10 promotes migration and invasion via the WNT-EMT signaling pathway in oral squamous cell carcinoma.

Journal of Cancer

Dai BW,Yang ZM,Deng P,Chen YR,He ZJ,Yang X,Zhang S,Wu HJ,Ren ZH

Published figure using Snail1 monoclonal antibody (Product # 53-9859-82) in Immunofluorescence

Wed Sep 30 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1

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Gene Aliases: dJ710H13.1; SLUGH2; SNA; SNAH; SNAI1; SNAIL; SNAIL1

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UniProt ID: (Human) O95863

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Entrez Gene ID: (Human) 6615

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding kinase binding metal ion binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation mesoderm formation epithelial to mesenchymal transition epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition negative regulation of vitamin D biosynthetic process cell migration positive regulation of cell migration hair follicle morphogenesis negative regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of transcription, DNA-templated palate development cartilage morphogenesis trophoblast giant cell differentiation negative regulation of cell differentiation involved in embryonic placenta development left/right pattern formation Notch signaling involved in heart development negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of bicellular tight junction assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Snail1 Monoclonal Antibody (20C8), Alexa Fluor™ 488, eBioscience™

Snail1 Antibody (53-9859-82) in ICC/IF

Product Details

53-9859-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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