Invitrogen

TIGIT Monoclonal Antibody (MBSA43), FITC, eBioscience™

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TIGIT Antibody (11-9500-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 eFluor® 450 (Product # 48-0047-42) and 0.125 µg of Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human TIGIT FITC (right). Total viable cells were used for analysis.

TIGIT Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-9500-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MBSA43

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572530

Product Specific Information

Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MBSA43 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The TIGIT⁺ T regulatory cells subset associates with nosocomial infection and fatal outcome in COVID-19 patients under mechanical ventilation.

Scientific reports

de Lima MHF,Machado CC,Nascimento DC,Silva CMS,Toller-Kawahisa JE,Rodrigues TS,Veras FP,Pontelli MC,Castro IA,Zamboni DS,Filho JA,Cunha TM,Arruda E,da Cunha LD,Oliveira RDR,Cunha FQ,Louzada-Junior P

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Mon Aug 21 00:00:00 EDT 2023

Impaired TIGIT expression on B cells drives circulating follicular helper T cell expansion in multiple sclerosis.

The Journal of clinical investigation

Asashima H,Axisa PP,Pham THG,Longbrake EE,Ruff WE,Lele N,Cohen I,Raddassi K,Sumida TS,Hafler DA

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Mon Oct 17 00:00:00 EDT 2022

Immune profile analysis of peripheral blood and tumors of lung cancer patients treated with immune checkpoint inhibitors.

Translational lung cancer research

Ichiki Y,Fukuyama T,Ueno M,Kanasaki Y,Goto H,Takahashi M,Mikami S,Kobayashi N,Nakanishi K,Hayashi S,Ishida T

11-9500-42 was used in Flow cytometry/Cell sorting to analyze the association between the immune-related molecular expression in peripheral blood mononuclear cells (PBMCs) and lung cancer tissues, and the effects of ICI monotherapy.

Tue Nov 01 00:00:00 EDT 2022

Immunometabolic analysis shows a distinct cyto-metabotype in Covid-19 compared to sepsis from other causes.

Heliyon

Trovato FM,Mujib S,Jerome E,Cavazza A,Morgan P,Smith J,Depante MT,O'Reilly K,Luxton J,Mare T,Napoli S,McPhail MJ

11-9500-42 was used in Flow cytometry/Cell sorting to support the hypothesis that IFN-γ -PD-L1 axis might be involved in the cytokine release syndrome typical of severe Covid-19 and the phenomenon persisted through multiple pandemic waves despite use of immunomodulation.

Wed Jun 01 00:00:00 EDT 2022

Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis.

Oncogenesis

Xu K,Wang R,Xie H,Hu L,Wang C,Xu J,Zhu C,Liu Y,Gao F,Li X,Wang C,Huang J,Zhou W,Zhou G,Shu Y,Guan X

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Immunocytochemistry

Tue Oct 05 00:00:00 EDT 2021

Correlations of the expression of γδ T cells and their co-stimulatory molecules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion.

Clinical and experimental immunology

Liang Q,Tong L,Xiang L,Shen S,Pan C,Liu C,Zhang H

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

TIGIT⁺ TIM-3⁺ NK cells are correlated with NK cell exhaustion and disease progression in patients with hepatitis B virus‑related hepatocellular carcinoma.

Oncoimmunology

Yu L,Liu X,Wang X,Yan F,Wang P,Jiang Y,Du J,Yang Z

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

SARS-CoV-2-specific T cell memory is sustained in COVID-19 convalescent patients for 10 months with successful development of stem cell-like memory T cells.

Nature communications

Jung JH,Rha MS,Sa M,Choi HK,Jeon JH,Seok H,Park DW,Park SH,Jeong HW,Choi WS,Shin EC

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Wed Jun 30 00:00:00 EDT 2021

Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID-19.

Journal of cellular and molecular medicine

Yan L,Cai B,Li Y,Wang MJ,An YF,Deng R,Li DD,Wang LC,Xu H,Gao XD,Wang LL

11-9500-42 was used in Flow Cytometry to study the changes of NK, T- and B-cell subsets, inflammatory cytokines and virus-specific antibodies in patients with moderate COVID-19. Subsets were analysed using flow cytometry, cytokines and SARS-CoV-2-specific antibodies were analysed using an electrochemiluminescence immunoassay.

Tue Dec 01 00:00:00 EST 2020

TIGIT is upregulated by HIV-1 infection and marks a highly functional adaptive and mature subset of natural killer cells.

AIDS (London, England)

Vendrame E,Seiler C,Ranganath T,Zhao NQ,Vergara R,Alary M,Labbé AC,Guédou F,Poudrier J,Holmes S,Roger M,Blish CA

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL.

Genome medicine

Wierzbinska JA,Toth R,Ishaque N,Rippe K,Mallm JP,Klett LC,Mertens D,Zenz T,Hielscher T,Seifert M,Küppers R,Assenov Y,Lutsik P,Stilgenbauer S,Roessner PM,Seiffert M,Byrd J,Oakes CC,Plass C,Lipka DB

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2020

Risk-associated alterations in marrow T cells in pediatric leukemia.

JCI insight

Bailur JK,McCachren SS,Pendleton K,Vasquez JC,Lim HS,Duffy A,Doxie DB,Kaushal A,Foster C,DeRyckere D,Castellino S,Kemp ML,Qiu P,Dhodapkar MV,Dhodapkar KM

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

A Comprehensive Analysis of Key Immune Checkpoint Receptors on Tumor-Infiltrating T Cells From Multiple Types of Cancer.

Frontiers in oncology

Li X,Wang R,Fan P,Yao X,Qin L,Peng Y,Ma M,Asley N,Chang X,Feng Y,Hu Y,Zhang Y,Li C,Fanning G,Jones S,Verrill C,Maldonado-Perez D,Sopp P,Waugh C,Taylor S,Mcgowan S,Cerundolo V,Conlon C,McMichael A,Lu S,Wang X,Li N,Dong T

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Sun Jan 10 00:00:00 EST 2021

OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Belkina AC,Snyder-Cappione JE

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

Published figure using TIGIT monoclonal antibody (Product # 11-9500-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule

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Gene Aliases: TIGIT; VSIG9; VSTM3; WUCAM

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UniProt ID: (Human) Q495A1

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Entrez Gene ID: (Human) 201633

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Function(s)

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIGIT Monoclonal Antibody (MBSA43), FITC, eBioscience™

TIGIT Antibody (11-9500-42) in Flow

Product Details

11-9500-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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