Invitrogen

TIGIT Monoclonal Antibody (MBSA43), PE, eBioscience™

FIGURE: 1 / 1

TIGIT Antibody (12-9500-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42), Anti-Human CD45RO PerCP-eFluor® 710 (Product # 46-0457-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human TIGIT PE (right). CD4+ cells in the lymphocyte gate were used for analysis.

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Product Details

12-9500-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MBSA43

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10714831

Product Specific Information

Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MBSA43 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis.

International journal of molecular medicine

Dong W,Li W,Zhang S,Zeng X,Qin Q,Fan H,Tang Z,Wu X,Lu G

12-9500-42 was used in Flow cytometry/Cell sorting to report an X-SCID patient with a de novo c.677C>T (p.R226H) variant in exon 5 of IL2RG, exhibiting recurrent and persistent infections from 3-months-old.

Wed Mar 01 00:00:00 EST 2023

Increased expression of TIGIT and KLRG1 correlates with impaired CD56^bright NK cell immunity in HPV16-related cervical intraepithelial neoplasia.

Virology journal

Nie Y,Liu D,Yang W,Li Y,Zhang L,Cheng X,Chen R,Yuan B,Zhang G,Wang H

12-9500-42 was used in Flow cytometry/Cell sorting to examine the potential role of natural killer (NK) cell non-MHC-I specific inhibitory receptors expression in immune escape from HPV16(+)cervical intraepithelial neoplasia (CIN) patients.

Tue Apr 12 00:00:00 EDT 2022

CRISPR/Cas9-Mediated Insertion of HIV Long Terminal Repeat within BACH2 Promotes Expansion of T Regulatory-like Cells.

Journal of immunology (Baltimore, Md. : 1950)

Christian ML,Dapp MJ,Scharffenberger SC,Jones H,Song C,Frenkel LM,Krumm A,Mullins JI,Rawlings DJ

12-9500-42 was used in Flow cytometry/Cell sorting to model the effects of HIV integration within BACH2 Integration of the HIV long terminal repeat (LTR) and major splice donor increased BACH2 mRNA and protein levels, altered gene expression, and promoted selective outgrowth of an activated, proliferative, and T regulatory-like cell population.

Fri Apr 01 00:00:00 EDT 2022

Humans with inherited T cell CD28 deficiency are susceptible to skin papillomaviruses but are otherwise healthy.

Cell

Béziat V,Rapaport F,Hu J,Titeux M,Bonnet des Claustres M,Bourgey M,Griffin H,Bandet É,Ma CS,Sherkat R,Rokni-Zadeh H,Louis DM,Changi-Ashtiani M,Delmonte OM,Fukushima T,Habib T,Guennoun A,Khan T,Bender N,Rahman M,About F,Yang R,Rao G,Rouzaud C,Li J,Shearer D,Balogh K,Al Ali F,Ata M,Dabiri S,Momenilandi M,Nammour J,Alyanakian MA,Leruez-Ville M,Guenat D,Materna M,Marcot L,Vladikine N,Soret C,Vahidnezhad H,Youssefian L,Saeidian AH,Uitto J,Catherinot É,Navabi SS,Zarhrate M,Woodley DT,Jeljeli M,Abraham T,Belkaya S,Lorenzo L,Rosain J,Bayat M,Lanternier F,Lortholary O,Zakavi F,Gros P,Orth G,Abel L,Prétet JL,Fraitag S,Jouanguy E,Davis MM,Tangye SG,Notarangelo LD,Marr N,Waterboer T,Langlais D,Doorbar J,Hovnanian A,Christensen N,Bossuyt X,Shahrooei M,Casanova JL

12-9500-42 was used in Flow cytometry/Cell sorting to conclude that human CD28-dependent T cell responses are largely redundant for protective immunity.

Thu Jul 08 00:00:00 EDT 2021

Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade.

Cancer cell

Srivastava S,Furlan SN,Jaeger-Ruckstuhl CA,Sarvothama M,Berger C,Smythe KS,Garrison SM,Specht JM,Lee SM,Amezquita RA,Voillet V,Muhunthan V,Yechan-Gunja S,Pillai SPS,Rader C,Houghton AM,Pierce RH,Gottardo R,Maloney DG,Riddell SR

12-9500-42 was used in Flow Cytometry to test strategies for enhancing efficacy of CAR-T cells targeting the tumor-associated antigen receptor tyrosine kinase-like orphan receptor 1 (ROR1) infiltrating tumors.

Mon Feb 08 00:00:00 EST 2021

Imbalance of the CD226/TIGIT Immune Checkpoint Is Involved in the Pathogenesis of Primary Biliary Cholangitis.

Frontiers in immunology

Deng C,Li W,Fei Y,Wang L,Chen Y,Zeng X,Zhang F,Li Y

12-9500 was used in Flow cytometry/Cell sorting to look at PBC patients (n = 42) showing significantly higher proportions of peripheral CD8+ T and CD4+ T cells expressing either CD226 or TIGIT than disease (n = 25) and healthy (n = 30) controls.

Mon Apr 19 00:00:00 EDT 2021

A Comprehensive Analysis of Key Immune Checkpoint Receptors on Tumor-Infiltrating T Cells From Multiple Types of Cancer.

Frontiers in oncology

Li X,Wang R,Fan P,Yao X,Qin L,Peng Y,Ma M,Asley N,Chang X,Feng Y,Hu Y,Zhang Y,Li C,Fanning G,Jones S,Verrill C,Maldonado-Perez D,Sopp P,Waugh C,Taylor S,Mcgowan S,Cerundolo V,Conlon C,McMichael A,Lu S,Wang X,Li N,Dong T

12-9500-42 was used in Flow Cytometry to investigate mono-blockade or combinatorial blockade regimens and how they may reinvigorate anti tumor T-cell immunity in those cancer patients while limiting immune-related adverse effects.

Sun Jan 10 00:00:00 EST 2021

A transcriptionally and functionally distinct PD-1⁺ CD8⁺ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.

Nature medicine

Thommen DS,Koelzer VH,Herzig P,Roller A,Trefny M,Dimeloe S,Kiialainen A,Hanhart J,Schill C,Hess C,Savic Prince S,Wiese M,Lardinois D,Ho PC,Klein C,Karanikas V,Mertz KD,Schumacher TN,Zippelius A

12-9500 was used in Flow cytometry/Cell sorting to compare transcriptional, metabolic and functional signatures of intratumoral CD8+ T lymphocyte populations with high, intermediate and no PD-1 expression from non-small-cell lung cancer patients.

Sun Jul 01 00:00:00 EDT 2018

Human Immunodeficiency Virus Type-1 Elite Controllers Maintain Low Co-Expression of Inhibitory Receptors on CD4+ T Cells.

Frontiers in immunology

Noyan K,Nguyen S,Betts MR,Sönnerborg A,Buggert M

12-9500 was used in Flow cytometry/Cell sorting to suggest that ELCs harbor a "healthy" state of inhibitory receptor expression on CD4+ T cells that might play part in maintenance of their control status.

Thu Feb 14 00:00:00 EST 2019

An Autocrine TNFα-Tumor Necrosis Factor Receptor 2 Loop Promotes Epigenetic Effects Inducing Human Treg Stability In Vitro.

Frontiers in immunology

Urbano PCM,Koenen HJPM,Joosten I,He X

12-9500 was used in Flow cytometry/Cell sorting to explore the underlying mechanisms of rapamycin and tumour necrosis factor receptor 2 agonist-mediated regulatory T cell stability.

Tue May 28 00:00:00 EDT 2019

Effect of analytical treatment interruption and reinitiation of antiretroviral therapy on HIV reservoirs and immunologic parameters in infected individuals.

PLoS pathogens

Clarridge KE,Blazkova J,Einkauf K,Petrone M,Refsland EW,Justement JS,Shi V,Huiting ED,Seamon CA,Lee GQ,Yu XG,Moir S,Sneller MC,Lichterfeld M,Chun TW

12-9500 was used in Flow cytometry/Cell sorting to evaluate if there's a correlation between analytical treatment interruption of ART in HIV patients, and the size of the persistent HIV reservoir.

Mon Jan 01 00:00:00 EST 2018

PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.

Frontiers in immunology

Stecher C,Battin C,Leitner J,Zettl M,Grabmeier-Pfistershammer K,Höller C,Zlabinger GJ,Steinberger P

Wed Mar 30 00:00:00 EDT 2022

Perturbed CD8⁺ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals.

Scientific reports

Tauriainen J,Scharf L,Frederiksen J,Naji A,Ljunggren HG,Sönnerborg A,Lund O,Reyes-Terán G,Hecht FM,Deeks SG,Betts MR,Buggert M,Karlsson AC

12950042 was used in flow cytometry to show T cell immunoglobulin and ITIM domain expression increases over time despite early initiation of antiretroviral treatment

Fri Jan 13 00:00:00 EST 2017

A cellular platform for the evaluation of immune checkpoint molecules.

Oncotarget

Jutz S,Hennig A,Paster W,Asrak Ö,Dijanovic D,Kellner F,Pickl WF,Huppa JB,Leitner J,Steinberger P

12-9500 was used in Flow cytometry/Cell sorting to evaluate the potential of fluorescence-based transcriptional reporters based on the human Jurkat T cell line in conjunction with engineered T cell stimulator cell lines for investigating coinhibitory pathways.

Tue Sep 12 00:00:00 EDT 2017

Immunological biomarkers predict HIV-1 viral rebound after treatment interruption.

Nature communications

Hurst J,Hoffmann M,Pace M,Williams JP,Thornhill J,Hamlyn E,Meyerowitz J,Willberg C,Koelsch KK,Robinson N,Brown H,Fisher M,Kinloch S,Cooper DA,Schechter M,Tambussi G,Fidler S,Babiker A,Weber J,Kelleher AD,Phillips RE,Frater J

12-9500 was used in Flow cytometry/Cell sorting to show that immunological biomarkers can predict time to viral rebound after stopping antiretroviral therapy.

Fri Oct 09 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule

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Gene Aliases: TIGIT; VSIG9; VSTM3; WUCAM

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UniProt ID: (Human) Q495A1

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Entrez Gene ID: (Human) 201633

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Function(s)

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIGIT Monoclonal Antibody (MBSA43), PE, eBioscience™

TIGIT Antibody (12-9500-42) in Flow

Product Details

12-9500-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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