Invitrogen

TNF alpha Monoclonal Antibody (MAb11), eBioscience™

TNF alpha Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 24

TNF alpha Antibody (14-7349-81) in IHC

Oncotarget 2020 - Figure 4 Effects of ghrelin on LLC-induced changes in inflammation and macrophages in BAT. HK+V: heat-killed + vehicle; T+V: tumor + vehicle; T+G: tumor + ghrelin. Protein levels of inflammatory markers ( A ) IL-1beta, ( B ) IL-6, and ( C ) TNF; and ( D ) macrophage marker MCP-1 in BAT (pg/mg). Two-way ANOVA was performed to detect genotype and treatment differences. * p < 0.05; ** p < 0.01; *** p < 0.001 c... View More

TNF alpha Antibody in Flow Cytometry (Flow)

TNF alpha Antibody in Flow Cytometry, Neutralization (Flow, Neu)

Product Image Gallery

Product Details

14-7349-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-Dependent

View 51 publications 51 publications

ELISA (ELISA)

View 3 publications 3 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MAb11

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468489

Product Specific Information

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: MAb11 has been reported for use in detection of human TNF alpha by ELISA. It can also be used for intracellular flow cytometric analysis.

Applications Tested: The biotinylated format for this lot of MAb11 has been tested by sandwich ELISA for the detection of human TNF alpha in combination with MAb1 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Fri Jun 16 00:00:00 EDT 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Thu Feb 03 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Thu Jan 21 00:00:00 EST 2021

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Immunity

Schwabenland M,Salié H,Tanevski J,Killmer S,Lago MS,Schlaak AE,Mayer L,Matschke J,Püschel K,Fitzek A,Ondruschka B,Mei HE,Boettler T,Neumann-Haefelin C,Hofmann M,Breithaupt A,Genc N,Stadelmann C,Saez-Rodriguez J,Bronsert P,Knobeloch KP,Blank T,Thimme R,Glatzel M,Prinz M,Bengsch B

14-7349-81 was used in Mass cytometry to identify profound neuroinflammation with activation of innate and adaptive immune cells as correlates of COVID-19 neuropathology, with implications for potential therapeutic strategies.

Tue Jul 13 00:00:00 EDT 2021

Membrane lymphotoxin-α₂β is a novel tumor necrosis factor (TNF) receptor 2 (TNFR2) agonist.

Cell death & disease

Kucka K,Lang I,Zhang T,Siegmund D,Medler J,Wajant H

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Apr 06 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized Mice.

Frontiers in immunology

Iwabuchi R,Ide K,Terahara K,Wagatsuma R,Iwaki R,Matsunaga H,Tsunetsugu-Yokota Y,Takeyama H,Takahashi Y

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2020

Ghrelin ameliorates tumor-induced adipose tissue atrophy and inflammation via Ghrelin receptor-dependent and -independent pathways.

Oncotarget

Liu H,Luo J,Guillory B,Chen JA,Zang P,Yoeli JK,Hernandez Y,Lee II,Anderson B,Storie M,Tewnion A,Garcia JM

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Immunohistochemistry

Tue Sep 01 00:00:00 EDT 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Lifetime encoding in flow cytometry for bead-based sensing of biomolecular interaction.

Scientific reports

Kage D,Hoffmann K,Borcherding H,Schedler U,Resch-Genger U

14-7349-81 was used in Flow Cytometry to demonstrate the potential of time-resolved flow cytometry (FCM) for bioanalysis, clinical diagnostics, and optically encoded bead-based assays.

Tue Nov 10 00:00:00 EST 2020

Immune Checkpoint Inhibitor-induced Reinvigoration of Tumor-infiltrating CD8⁺ T Cells is Determined by Their Differentiation Status in Glioblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Park J,Kwon M,Kim KH,Kim TS,Hong SH,Kim CG,Kang SG,Moon JH,Kim EH,Park SH,Chang JH,Shin EC

14-7349 was used in Flow cytometry/Cell sorting to examine the differentiation status of CD8+ tumor-infiltrating lymphocytes (TIL) from patients with primary GBM and their reinvigoration by ICIs to understand the nature of T-cell exhaustion in GBM.

Mon Apr 15 00:00:00 EDT 2019

Chemokine receptor CCR5 correlates with functional CD8⁺ T cells in SIV-infected macaques and the potential effects of maraviroc on T-cell activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang X,Russell-Lodrigue KE,Ratterree MS,Veazey RS,Xu H

14-7349-81 was used in Flow Cytometry to investigate the the function of CCR5 on CD8 T cells.

Thu Aug 01 00:00:00 EDT 2019

Cytomegalovirus-Driven Adaption of Natural Killer Cells in NKG2C^null Human Immunodeficiency Virus-Infected Individuals.

Viruses

Comeau EM,Holder KA,Fudge NJ,Grant MD

14-7349-81 was used in Flow Cytometry to investigate whether the loss of NKG2C expression affects natural killer cell acquisition of superior antibody-dependent function in the diminished resistance to HIV infection seen with this loss.

Sat Mar 09 00:00:00 EST 2019

Inhibition of histone H3K27 demethylases selectively modulates inflammatory phenotypes of natural killer cells.

The Journal of biological chemistry

Cribbs A,Hookway ES,Wells G,Lindow M,Obad S,Oerum H,Prinjha RK,Athanasou N,Sowman A,Philpott M,Penn H,Soderstrom K,Feldmann M,Oppermann U

14-7349 was used in Flow cytometry/Cell sorting to indicate that H3K27me3 is a dynamic and important epigenetic modification during NK cell activation and that JMJD3/UTX-driven H3K27 demethylation is critical for NK cell function.

Fri Feb 16 00:00:00 EST 2018

Heterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.

Cell

Jiménez-Sánchez A,Memon D,Pourpe S,Veeraraghavan H,Li Y,Vargas HA,Gill MB,Park KJ,Zivanovic O,Konner J,Ricca J,Zamarin D,Walther T,Aghajanian C,Wolchok JD,Sala E,Merghoub T,Snyder A,Miller ML

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Thu Aug 24 00:00:00 EDT 2017

Fibroblast-adapted human CMV vaccines elicit predominantly conventional CD8 T cell responses in humans.

The Journal of experimental medicine

Murray SE,Nesterenko PA,Vanarsdall AL,Munks MW,Smart SM,Veziroglu EM,Sagario LC,Lee R,Claas FHJ,Doxiadis IIN,McVoy MA,Adler SP,Hill AB

14-7349 was used in Flow cytometry/Cell sorting to describe the CD8 T cell responses of human subjects vaccinated with two fibroblast-adapted HCMV vaccines.

Mon Jul 03 00:00:00 EDT 2017

ICOS⁺ Foxp3⁺ TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.

International journal of cancer

Nagase H,Takeoka T,Urakawa S,Morimoto-Okazawa A,Kawashima A,Iwahori K,Takiguchi S,Nishikawa H,Sato E,Sakaguchi S,Mori M,Doki Y,Wada H

14734981 was used in flow cytometry to establish the T Cell receptor-inducible costimulator as a promising target for direct T regulatory cell-targeting therapeutic agents for gastric cancer

Wed Feb 01 00:00:00 EST 2017

Clinical impact of NK-cell reconstitution after reduced intensity conditioned unrelated cord blood transplantation in patients with acute myeloid leukemia: analysis of a prospective phase II multicenter trial on behalf of the Société Française de Greffe de Moelle Osseuse et Thérapie Cellulaire and Eurocord.

Bone marrow transplantation

Nguyen S,Achour A,Souchet L,Vigouroux S,Chevallier P,Furst S,Sirvent A,Bay JO,Socié G,Ceballos P,Huynh A,Cornillon J,Francois S,Legrand F,Yakoub-Agha I,Michel G,Maillard N,Margueritte G,Maury S,Uzunov M,Bulabois CE,Michallet M,Clement L,Dauriac C,Bilger K,Lejeune J,Béziat V,Rocha V,Rio B,Chevret S,Vieillard V

14-7349 was used in Flow cytometry/Cell sorting to reflect a general impairment of the NK licensing process in HLA-mismatched HSCT.

Sun Oct 01 00:00:00 EDT 2017

Optimization of mass cytometry sample cryopreservation after staining.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Sumatoh HR,Teng KW,Cheng Y,Newell EW

14734981 was used in flow cytometry to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment

Sun Jan 01 00:00:00 EST 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

A pilot study showing associations between frequency of CD4(+) memory cell subsets at diagnosis and duration of partial remission in type 1 diabetes.

Clinical immunology (Orlando, Fla.)

Moya R,Robertson HK,Payne D,Narsale A,Koziol J,Davies JD

14-7349-81 was used in Flow Cytometry to assess whether frequencies of CD4(+) T cell subsets in children newly diagnosed with type 1 diabetes are associated with length of partial remission.

Sun May 01 00:00:00 EDT 2016

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Infection and immunity

Phuah J,Wong EA,Gideon HP,Maiello P,Coleman MT,Hendricks MR,Ruden R,Cirrincione LR,Chan J,Lin PL,Flynn JL

14734981 was used in flow cytometry to report that B cells modulate the local granulomatous response in Mycobacterium tuberculosis-infected macaques during acute infection

Sun May 01 00:00:00 EDT 2016

Accumulation of Intrahepatic TNF-α-Producing NKp44+ NK Cells Correlates With Liver Fibrosis and Viral Load in Chronic HCV Infection.

Medicine

Nel I,Lucar O,Petitdemange C,Béziat V,Lapalus M,Bédossa P,Debré P,Asselah T,Marcellin P,Vieillard V

14-7349 was used in Flow cytometry/Cell sorting to suggest that accumulation of intrahepatic tumor necrosis factor-α-producing NKp44 resident NK cells correlates with liver damage during chronic HCV infection.

Sun May 01 00:00:00 EDT 2016

MLN4924 sensitizes monocytes and maturing dendritic cells for TNF-dependent and -independent necroptosis.

British journal of pharmacology

El-Mesery M,Seher A,Stühmer T,Siegmund D,Wajant H

14-7349 was used in Flow cytometry/Cell sorting to reveal a strong dual suppressive effect of MLN4924 on dendritic cell activity.

Sun Mar 01 00:00:00 EST 2015

Higher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers.

PloS one

Taborda NA,González SM,Alvarez CM,Correa LA,Montoya CJ,Rugeles MT

14-7349 was used in Flow cytometry/Cell sorting to investigate the frequency, phenotype and activity of T and NK cells in HIV controllers, showing higher frequencies of NK and CD4+ T-Cells and potent cytotoxic response.

Fri May 13 00:00:00 EDT 2016

Impaired Th17 polarization of phenotypically naive CD4(+) T-cells during chronic HIV-1 infection and potential restoration with early ART.

Retrovirology

DaFonseca S,Niessl J,Pouvreau S,Wacleche VS,Gosselin A,Cleret-Buhot A,Bernard N,Tremblay C,Jenabian MA,Routy JP,Ancuta P

14734981 was used in flow cytometry to elucidate Th17 cell polarization, depletion, and restoration in response to HIV infection and antiretroviral therapy

Thu Apr 30 00:00:00 EDT 2015

Inflammatory Signaling by NOD-RIPK2 Is Inhibited by Clinically Relevant Type II Kinase Inhibitors.

Chemistry & biology

Canning P,Ruan Q,Schwerd T,Hrdinka M,Maki JL,Saleh D,Suebsuwong C,Ray S,Brennan PE,Cuny GD,Uhlig HH,Gyrd-Hansen M,Degterev A,Bullock AN

14-7349 was used in Flow cytometry/Cell sorting to demonstrate the potential for type II inhibitors to treat indications of RIPK2 activation as well as inflammation-associated cancers.

Thu Sep 17 00:00:00 EDT 2015

Signaling lymphocytic activation molecule (SLAM)/SLAM-associated protein pathway regulates human B-cell tolerance.

The Journal of allergy and clinical immunology

Menard L,Cantaert T,Chamberlain N,Tangye SG,Riminton S,Church JA,Klion A,Cunningham-Rundles C,Nichols KE,Meffre E

14-7349 was used in Flow cytometry/Cell sorting to show SLAM-associated protein expression is necessary for the counterselection of developing autoreactive B cells and prevents their T cell-dependent accumulation in the periphery.

Tue Apr 01 00:00:00 EDT 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

T cell inactivation by poxviral B22 family proteins increases viral virulence.

PLoS pathogens

Alzhanova D,Hammarlund E,Reed J,Meermeier E,Rawlings S,Ray CA,Edwards DM,Bimber B,Legasse A,Planer S,Sprague J,Axthelm MK,Pickup DJ,Lewinsohn DM,Gold MC,Wong SW,Sacha JB,Slifka MK,Früh K

14-7349 was used in Flow cytometry/Cell sorting to conclude that B22 family proteins cause viral virulence by suppressing T cell control of viral dissemination.

Thu May 01 00:00:00 EDT 2014

T cell inactivation by poxviral B22 family proteins increases viral virulence.

PLoS pathogens

Alzhanova D,Hammarlund E,Reed J,Meermeier E,Rawlings S,Ray CA,Edwards DM,Bimber B,Legasse A,Planer S,Sprague J,Axthelm MK,Pickup DJ,Lewinsohn DM,Gold MC,Wong SW,Sacha JB,Slifka MK,Früh K

14-7349 was used in Flow cytometry/Cell sorting to conclude that B22 family proteins cause viral virulence by suppressing T cell control of viral dissemination.

Thu May 01 00:00:00 EDT 2014

Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis.

Rheumatology (Oxford, England)

Todd SK,Pepper RJ,Draibe J,Tanna A,Pusey CD,Mauri C,Salama AD

14-7349 was used in Immunohistochemistry to study the frequencies of different B cell subtypes during progression of ANCA-associated vasculitis.

Mon Sep 01 00:00:00 EDT 2014

Microglial derived tumor necrosis factor-α drives Alzheimer's disease-related neuronal cell cycle events.

Neurobiology of disease

Bhaskar K,Maphis N,Xu G,Varvel NH,Kokiko-Cochran ON,Weick JP,Staugaitis SM,Cardona A,Ransohoff RM,Herrup K,Lamb BT

14-7349 was used in Functional assays to suggest a cell-autonomous role of microglia, and identify TNFα as the responsible cytokine, in promoting neuronal cell cycle events in the pathogenesis of AD.

Sat Feb 01 00:00:00 EST 2014

A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251.

Journal of virology

Van Rompay KK,Hunter Z,Jayashankar K,Peabody J,Montefiori D,LaBranche CC,Keele BF,Jensen K,Abel K,Chackerian B

14-7349 was used in Flow cytometry/Cell sorting to investigate whether prophylactic immunisation of rhesus macaques with a vaccine against CCR5 could prevent or suppress SIV.

Sat Feb 01 00:00:00 EST 2014

HTLV-1 bZIP factor-specific CD4 T cell responses in adult T cell leukemia/lymphoma patients after allogeneic hematopoietic stem cell transplantation.

Journal of immunology (Baltimore, Md. : 1950)

Narita T,Ishida T,Masaki A,Suzuki S,Ito A,Mori F,Yamada T,Ri M,Kusumoto S,Komatsu H,Miyazaki Y,Takatsuka Y,Utsunomiya A,Niimi A,Iida S,Ueda R

14734981 was used in flow cytometry to investigate human T lymphotropic virus type 1 bZIP factor-specific CD4 T cell responses

Sat Feb 01 00:00:00 EST 2014

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

14734981 was used in flow cytometry to investigate the impact of CCR6(+)CXCR3(+)CCR4(-) cells in latent tuberculosis infection

Mon Sep 15 00:00:00 EDT 2014

Cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age.

Human vaccines & immunotherapeutics

Geevarghese B,Weinberg A

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Mon Apr 25 00:00:00 EDT 2016

Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.

BioMed research international

Lopes DM,Fernandes JS,Cardoso TM,Bafica AM,Oliveira SC,Carvalho EM,Araujo MI,Cardoso LS

14-7349 was used in Flow cytometry/Cell sorting to investigate the dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.

Thu Jul 02 00:00:00 EDT 2015

Monocytes from chronic HBV patients react in vitro to HBsAg and TLR by producing cytokines irrespective of stage of disease.

PloS one

Boltjes A,Groothuismink ZM,van Oord GW,Janssen HL,Woltman AM,Boonstra A

14-7349 was used in Flow cytometry/Cell sorting to investigate the in vitro and in vivo effects of hepatitis B virus surface antigen on monocytes from chronic HBV patients.

Tue Jan 06 00:00:00 EST 2015

Phase I trial of a multi-epitope-pulsed dendritic cell vaccine for patients with newly diagnosed glioblastoma.

Cancer immunology, immunotherapy : CII

Phuphanich S,Wheeler CJ,Rudnick JD,Mazer M,Wang H,Nuño MA,Richardson JE,Fan X,Ji J,Chu RM,Bender JG,Hawkins ES,Patil CG,Black KL,Yu JS

14-7349 was used in Flow cytometry/Cell sorting to study how expression of four ICT-107 targeted antigens in the pre-vaccine tumors correlated with prolonged overall survival and PFS in ND-GBM patients.

Tue Jan 01 00:00:00 EST 2013

Immune activation and regulation in simian immunodeficiency virus-Plasmodium fragile-coinfected rhesus macaques.

Journal of virology

Trott KA,Richardson A,Hudgens MA,Abel K

14-7349 was used in Flow cytometry/Cell sorting to investigate how immune activation and elevated IL-10 interacts in HIV and malaria coinfected individuals.

Sun Sep 01 00:00:00 EDT 2013

Functional OCT4-specific CD4⁺ and CD8⁺ T cells in healthy controls and ovarian cancer patients.

Oncoimmunology

Di J,Massuger LF,Duiveman-de Boer T,Zusterzeel PL,Figdor CG,Torensma R

Published figure using TNF alpha monoclonal antibody (Product # 14-7349-81) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

14-7349 was used in Flow cytometry/Cell sorting to investigate the role of the PI3K-PKB-mTOR axis in human plasmacytoid dendritic cell development.

Thu Dec 13 00:00:00 EST 2012

The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.

Journal of leukocyte biology

Nijnik A,Pistolic J,Cho P,Filewod NC,Falsafi R,Ramin A,Harder KW,Hancock RE

14-7349 was used in an ELISA assay to elucidate the common molecular mechanisms mediating the chemokine induction activity of natural and synthetic cationic peptides in monocytic cells.

Sun Apr 01 00:00:00 EDT 2012

The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation.

Infection and immunity

Gellatly SL,Needham B,Madera L,Trent MS,Hancock RE

14-7349 was used in an ELISA assay to assess how disruption of the regulatory system, PhoPQ, in gram-negative species of bacteria affects virulence.

Sat Sep 01 00:00:00 EDT 2012

STAT3 regulates monocyte TNF-alpha production in systemic inflammation caused by cardiac surgery with cardiopulmonary bypass.

PloS one

de Jong PR,Schadenberg AW,van den Broek T,Beekman JM,van Wijk F,Coffer PJ,Prakken BJ,Jansen NJ

14-7349 was used in Flow cytometry/Cell sorting to implicate STAT3 as a potential molecular target for pharmacological interventions in syndromes with systemic inflammation.

Wed Oct 17 00:00:00 EDT 2012

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells.

Nature medicine

Ma C,Fan R,Ahmad H,Shi Q,Comin-Anduix B,Chodon T,Koya RC,Liu CC,Kwong GA,Radu CG,Ribas A,Heath JR

14-7349 was used in Flow cytometry/Cell sorting to develop a microchip for quantitative measurement of secreted proteins from single cells.

Wed Jun 01 00:00:00 EDT 2011

Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.

Journal of the American Chemical Society

Luchansky MS,Bailey RC

14-7349 was used in Chromatin immunoprecipitation to profile cellular secretion using silicon photonic microring resonator arrays.

Wed Dec 21 00:00:00 EST 2011

Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL

14-7349 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model.

Tue Mar 15 00:00:00 EDT 2011

Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL

14-7349 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model.

Tue Mar 15 00:00:00 EDT 2011

Loss of IL-17-producing CD8 T cells during late chronic stage of pathogenic simian immunodeficiency virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Nigam P,Kwa S,Velu V,Amara RR

14-7349 was used in Flow cytometry/Cell sorting to characterise the anatomical distribution, phenotype, and functional quality of Tc17 and Th17 cells in SIV- and SIV+ rhesus macaques.

Sat Jan 15 00:00:00 EST 2011

Loss of IL-17-producing CD8 T cells during late chronic stage of pathogenic simian immunodeficiency virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Nigam P,Kwa S,Velu V,Amara RR

14-7349 was used in Flow cytometry/Cell sorting to characterise the anatomical distribution, phenotype, and functional quality of Tc17 and Th17 cells in SIV- and SIV+ rhesus macaques.

Sat Jan 15 00:00:00 EST 2011

Cowpox virus inhibits the transporter associated with antigen processing to evade T cell recognition.

Cell host & microbe

Alzhanova D,Edwards DM,Hammarlund E,Scholz IG,Horst D,Wagner MJ,Upton C,Wiertz EJ,Slifka MK,Früh K

14-7349 was used in Flow cytometry/Cell sorting to identify cowpox virus proteins that mediate T cell evasion in multiple hosts.

Thu Nov 19 00:00:00 EST 2009

GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine.

Virology

Lai L,Vödrös D,Kozlowski PA,Montefiori DC,Wilson RL,Akerstrom VL,Chennareddi L,Yu T,Kannanganat S,Ofielu L,Villinger F,Wyatt LS,Moss B,Amara RR,Robinson HL

14734981 was used in flow cytometry to assess the effects of using GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus-89.6 vaccine

Wed Dec 05 00:00:00 EST 2007

Bioinformatics

Protein Aliases: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

View more View less

Gene Aliases: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

View more View less

UniProt ID: (Human) P01375

View more View less

Entrez Gene ID: (Human) 7124

View more View less

Function(s)

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

TNF alpha Monoclonal Antibody (MAb11), eBioscience™

TNF alpha Antibody (14-7349-81) in IHC

Product Details

14-7349-81

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!