Invitrogen

TNF alpha Monoclonal Antibody (MAb11), PE-Cyanine7, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 26

TNF alpha Antibody (25-7349-82) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor® 710 (Product # 46-0047-42) and Anti-Human TNF alpha PE-Cyanine7 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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Product Details

25-7349-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 43 publications 43 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAb11

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469686

Product Specific Information

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported:This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Fri Jun 16 00:00:00 EDT 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Thu Feb 03 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

25-7349 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Thu Jan 21 00:00:00 EST 2021

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

25-7349 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Thu Jan 21 00:00:00 EST 2021

Membrane lymphotoxin-α₂β is a novel tumor necrosis factor (TNF) receptor 2 (TNFR2) agonist.

Cell death & disease

Kucka K,Lang I,Zhang T,Siegmund D,Medler J,Wajant H

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Tue Apr 06 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized Mice.

Frontiers in immunology

Iwabuchi R,Ide K,Terahara K,Wagatsuma R,Iwaki R,Matsunaga H,Tsunetsugu-Yokota Y,Takeyama H,Takahashi Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy.

Cell reports. Medicine

Müller TR,Jarosch S,Hammel M,Leube J,Grassmann S,Bernard B,Effenberger M,Andrä I,Chaudhry MZ,Käuferle T,Malo A,Cicin-Sain L,Steinberger P,Feuchtinger T,Protzer U,Schumann K,Neuenhahn M,Schober K,Busch DH

25-7349-82 was used in Immunocytochemistry to conclude that increased T cell product homogeneity after targeted TCR gene editing correlates with predictable in vivo T cell responses, which represents a crucial aspect for clinical application in adoptive T cell immunotherapy.

Tue Aug 17 00:00:00 EDT 2021

A controlled human Schistosoma mansoni infection model to advance novel drugs, vaccines and diagnostics.

Nature medicine

Langenberg MCC,Hoogerwerf MA,Koopman JPR,Janse JJ,Kos-van Oosterhoud J,Feijt C,Jochems SP,de Dood CJ,van Schuijlenburg R,Ozir-Fazalalikhan A,Manurung MD,Sartono E,van der Beek MT,Winkel BMF,Verbeek-Menken PH,Stam KA,van Leeuwen FWB,Meij P,van Diepen A,van Lieshout L,van Dam GJ,Corstjens PLAM,Hokke CH,Yazdanbakhsh M,Visser LG,Roestenberg M

25-7349 was used in Flow cytometry/Cell sorting to performed a dose-escalating clinical safety trial in 17 volunteers with male Schistosoma mansoni cercariae, which do not produce eggs (clinicaltrials.gov NCT02755324), at the Leiden University Medical Center, the Netherlands.

Sun Mar 01 00:00:00 EST 2020

Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity.

Cell

Rydyznski Moderbacher C,Ramirez SI,Dan JM,Grifoni A,Hastie KM,Weiskopf D,Belanger S,Abbott RK,Kim C,Choi J,Kato Y,Crotty EG,Kim C,Rawlings SA,Mateus J,Tse LPV,Frazier A,Baric R,Peters B,Greenbaum J,Ollmann Saphire E,Smith DM,Sette A,Crotty S

25-7349 was used in Flow cytometry/Cell sorting to complete a combined examination of all three branches of adaptive immunity at the level of SARS-CoV-2-specific CD4+ and CD8+ T cell and neutralizing antibody responses in acute and convalescent subjects.

Thu Nov 12 00:00:00 EST 2020

Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals.

Cell

Grifoni A,Weiskopf D,Ramirez SI,Mateus J,Dan JM,Moderbacher CR,Rawlings SA,Sutherland A,Premkumar L,Jadi RS,Marrama D,de Silva AM,Frazier A,Carlin AF,Greenbaum JA,Peters B,Krammer F,Smith DM,Crotty S,Sette A

25-7349 was used in Flow cytometry/Cell sorting to analyze the immune cell responses to SARS-CoV-2 of recovered patients to identify regions of the virus that are targeted and reveal cross-reactivity with other common circulating coronaviruses.

Thu Jun 25 00:00:00 EDT 2020

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

25-7349 was used in Flow cytometry/Cell sorting to eliminate T cell's HLA through the CRISPR/Cas9 gene editing system to evade donor HLA-mediated immune rejection.

Tue Oct 20 00:00:00 EDT 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

25-7349 was used in Flow cytometry/Cell sorting to eliminate T cell's HLA through the CRISPR/Cas9 gene editing system to evade donor HLA-mediated immune rejection.

Tue Oct 20 00:00:00 EDT 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

bioRxiv : the preprint server for biology

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MY,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Weiskopf D,Filev PD,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

25-7349 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Wed Sep 16 00:00:00 EDT 2020

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

bioRxiv : the preprint server for biology

25-7349 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Wed Sep 16 00:00:00 EDT 2020

Characterization of Antigenic MHC-Class-I-Restricted T Cell Epitopes in the Glycoprotein of Ebolavirus.

Cell reports

Powlson J,Wright D,Zeltina A,Giza M,Nielsen M,Rampling T,Venkatrakaman N,Bowden TA,Hill AVS,Ewer KJ

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Tue Nov 26 00:00:00 EST 2019

NK cells produce high levels of IL-10 early after allogeneic stem cell transplantation and suppress development of acute GVHD.

European journal of immunology

Chan YLT,Zuo J,Inman C,Croft W,Begum J,Croudace J,Kinsella F,Maggs L,Nagra S,Nunnick J,Abbotts B,Craddock C,Malladi R,Moss P

25-7349 was used in Flow cytometry/Cell sorting to reveal that robust reconstitution of immunoregulatory natural killer cells by day 14 after allogenic stem cell transplantation is an important determinant of the clinical outcome.

Thu Feb 01 00:00:00 EST 2018

Optimization of Large-Scale Expansion and Cryopreservation of Human Natural Killer Cells for Anti-Tumor Therapy.

Immune network

Min B,Choi H,Her JH,Jung MY,Kim HJ,Jung MY,Lee EK,Cho SY,Hwang YK,Shin EC

25-7349 was used in Flow cytometry/Cell sorting to assess the effect of cryopreservation on expanded natural killer cells in regards to viability, phenotype, and anti-tumour activity.

Wed Aug 01 00:00:00 EDT 2018

Unique features and clinical importance of acute alloreactive immune responses.

JCI insight

Inman CF,Eldershaw SA,Croudace JE,Davies NJ,Sharma-Oates A,Rai T,Pearce H,Sirovica M,Chan YLT,Verma K,Zuo J,Nagra S,Kinsella F,Nunnick J,Amel-Kashipaz R,Craddock C,Malladi R,Moss P

25-7349 was used in Flow cytometry/Cell sorting to study the effect of the allogeneic environment on T cells within the first 2 weeks after allogeneic stem cell transplantation.

Thu May 17 00:00:00 EDT 2018

Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical Trials.

Vaccines

Bowyer G,Rampling T,Powlson J,Morter R,Wright D,Hill AVS,Ewer KJ

25-7349 was used in Flow cytometry/Cell sorting to compare the use of markers for their sensitivity, specificity and associations with other measures of vaccine immunogenicity and demonstrate these as an additional method of reporting the T cell response to vaccination.

Tue Jul 31 00:00:00 EDT 2018

PD-1 Expression on Mycobacterium tuberculosis-Specific CD4 T Cells Is Associated With Bacterial Load in Human Tuberculosis.

Frontiers in immunology

Day CL,Abrahams DA,Bunjun R,Stone L,de Kock M,Walzl G,Wilkinson RJ,Burgers WA,Hanekom WA

25-7349 was used in Immunocytochemistry to study the effect of PD-1 expression on bacterial load and functional capacity of Mycobacterial tuberculosis specific T cell responses.

Mon Sep 30 00:00:00 EDT 2019

Heterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.

Cell

Jiménez-Sánchez A,Memon D,Pourpe S,Veeraraghavan H,Li Y,Vargas HA,Gill MB,Park KJ,Zivanovic O,Konner J,Ricca J,Zamarin D,Walther T,Aghajanian C,Wolchok JD,Sala E,Merghoub T,Snyder A,Miller ML

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Thu Aug 24 00:00:00 EDT 2017

Safety and Immunogenicity of Malaria Vectored Vaccines Given with Routine Expanded Program on Immunization Vaccines in Gambian Infants and Neonates: A Randomized Controlled Trial.

Frontiers in immunology

Mensah VA,Roetynck S,Kanteh EK,Bowyer G,Ndaw A,Oko F,Bliss CM,Jagne YJ,Cortese R,Nicosia A,Roberts R,D'Alessio F,Leroy O,Faye B,Kampmann B,Cisse B,Bojang K,Gerry S,Viebig NK,Lawrie AM,Clarke E,Imoukhuede EB,Ewer KJ,Hill AVS,Afolabi MO

25-7349 was used in Flow cytometry/Cell sorting to demonstrate malaria vectored prime-boost vaccines co-administered with routine childhood immunizations were well tolerated. Potent humoral and cellular immunity induced by ChAd63 MVA ME-TRAP did not reduce the immunogenicity of co-administered EPI vaccines, supporting further evaluation of this regimen in infant populations.

Mon Mar 14 00:00:00 EDT 2022

HIV-1 Infection Is Associated with Depletion and Functional Impairment of Mycobacterium tuberculosis-Specific CD4 T Cells in Individuals with Latent Tuberculosis Infection.

Journal of immunology (Baltimore, Md. : 1950)

Day CL,Abrahams DA,Harris LD,van Rooyen M,Stone L,de Kock M,Hanekom WA

25-7349 was used in Flow cytometry/Cell sorting to provide new insights into the parameters of M. tuberculosis-specific CD4 T cell immunity that are impaired in HIV-infected individuals with LTBI, which may contribute to their increased risk of developing active tuberculosis disease.

Fri Sep 15 00:00:00 EDT 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Wed Oct 18 00:00:00 EDT 2017

Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of γδ T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea.

BMC medicine

Schofield L,Ioannidis LJ,Karl S,Robinson LJ,Tan QY,Poole DP,Betuela I,Hill DL,Siba PM,Hansen DS,Mueller I,Eriksson EM

25-7349 was used in Flow cytometry/Cell sorting to demonstrate a novel role for IL-12/IL-18 in shaping the innate immune response and provide fundamental insight into aspects of γδ T cell immunoregulation.

Thu Jun 15 00:00:00 EDT 2017

IL-21 and probiotic therapy improve Th17 frequencies, microbial translocation, and microbiome in ARV-treated, SIV-infected macaques.

Mucosal immunology

Ortiz AM,Klase ZA,DiNapoli SR,Vujkovic-Cvijin I,Carmack K,Perkins MR,Calantone N,Vinton CL,Riddick NE,Gallagher J,Klatt NR,McCune JM,Estes JD,Paiardini M,Brenchley JM

25-7349 was used in Flow cytometry/Cell sorting to investigate methods to promote Th17 cell recovery in individuals infected with HIV, showing that IL-21 and probiotic therapy improve Th17 frequencies, microbial translocation, and microbiome in antiretroviral-treated, SIV-infected macaques.

Tue Mar 01 00:00:00 EST 2016

Long-lasting multifunctional CD8⁺ T cell responses in end-stage melanoma patients can be induced by dendritic cell vaccination.

Oncoimmunology

Wimmers F,Aarntzen EH,Duiveman-deBoer T,Figdor CG,Jacobs JF,Tel J,de Vries IJ

25-7349 was used in Flow cytometry/Cell sorting to investigate the functionality of tumour-specific CD8(+) T cells in melanoma patients upon dendritic cell vaccination, showing long-lasting multifunctional responses in end-stage patients.

Sat Jan 29 00:00:00 EST 2022

Macrophages Are Phenotypically and Functionally Diverse across Tissues in Simian Immunodeficiency Virus-Infected and Uninfected Asian Macaques.

Journal of virology

Ortiz AM,DiNapoli SR,Brenchley JM

25-7349 was used in Flow cytometry/Cell sorting to demonstrate that macrophages from healthy animals exhibit phenotypic and functional heterogeneity across tissues and across a variety of stimuli.

Mon Jun 01 00:00:00 EDT 2015

A randomized clinical trial in adults and newborns in South Africa to compare the safety and immunogenicity of bacille Calmette-Guérin (BCG) vaccine administration via a disposable-syringe jet injector to conventional technique with needle and syringe.

Vaccine

Geldenhuys HD,Mearns H,Foster J,Saxon E,Kagina B,Saganic L,Jarrahian C,Tameris MD,Dintwe OB,Van Rooyen M,Luabeya KK,Hussey G,Scriba TJ,Hatherill M,Zehrung D

25-7349 was used in Flow cytometry/Cell sorting to compare the safety and immunogenicity of BCG administration via the DSJI and NS techniques in adults and newborn infants.

Tue Sep 08 00:00:00 EDT 2015

Qualification of a whole blood intracellular cytokine staining assay to measure mycobacteria-specific CD4 and CD8 T cell immunity by flow cytometry.

Journal of immunological methods

Kagina BM,Mansoor N,Kpamegan EP,Penn-Nicholson A,Nemes E,Smit E,Gelderbloem S,Soares AP,Abel B,Keyser A,Sidibana M,Hughes JE,Kaplan G,Hussey GD,Hanekom WA,Scriba TJ

25-7349 was used in Flow cytometry/Cell sorting to qualify the intracellular cytokine staining process and to assess the effects of long-term cryopreservation.

Sun Feb 01 00:00:00 EST 2015

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Tue Aug 12 00:00:00 EDT 2014

CD40L-adjuvanted DNA/modified vaccinia virus Ankara simian immunodeficiency virus SIV239 vaccine enhances SIV-specific humoral and cellular immunity and improves protection against a heterologous SIVE660 mucosal challenge.

Journal of virology

Kwa S,Lai L,Gangadhara S,Siddiqui M,Pillai VB,Labranche C,Yu T,Moss B,Montefiori DC,Robinson HL,Kozlowski PA,Amara RR

25-7349 was used in Immunocytochemistry to highlight the benefits of CD40L adjuvant for enhancing antiviral humoral and cellular immunity against a pathogenic SIV.

Mon Sep 01 00:00:00 EDT 2014

Monocyte subsets in schistosomiasis patients with periportal fibrosis.

Mediators of inflammation

Fernandes JS,Araujo MI,Lopes DM,de Souza Rda P,Carvalho EM,Cardoso LS

25-7349 was used in Flow cytometry/Cell sorting to suggest that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis.

Mon Dec 15 00:00:00 EST 2014

Cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age.

Human vaccines & immunotherapeutics

Geevarghese B,Weinberg A

Published figure using TNF alpha monoclonal antibody (Product # 25-7349-82) in Flow Cytometry

Mon Apr 25 00:00:00 EDT 2016

Functional OCT4-specific CD4⁺ and CD8⁺ T cells in healthy controls and ovarian cancer patients.

Oncoimmunology

Di J,Massuger LF,Duiveman-de Boer T,Zusterzeel PL,Figdor CG,Torensma R

Wed May 01 00:00:00 EDT 2013

Mutational analysis identifies residues crucial for homodimerization of myeloid differentiation factor 88 (MyD88) and for its function in immune cells.

The Journal of biological chemistry

Loiarro M,Volpe E,Ruggiero V,Gallo G,Furlan R,Maiorino C,Battistini L,Sette C

25-7349 was used in Flow cytometry/Cell sorting to identify the novel residues of the Toll/IL-1 receptor (TIR) domain that are crucially involved in Myeloid differentiation factor 88 (MyD88) homodimerisation and TLR signalling.

Fri Oct 18 00:00:00 EDT 2013

Cancer/testis antigens are novel targets of immunotherapy for adult T-cell leukemia/lymphoma.

Blood

Nishikawa H,Maeda Y,Ishida T,Gnjatic S,Sato E,Mori F,Sugiyama D,Ito A,Fukumori Y,Utsunomiya A,Inagaki H,Old LJ,Ueda R,Sakaguchi S

25-7349 was used in Flow cytometry/Cell sorting to investigate the possible expression of cancer/testis antigens by adult T-cell leukemia/lymphoma cells.

Thu Mar 29 00:00:00 EDT 2012

Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis.

Journal of immunological methods

Vicetti Miguel RD,Maryak SA,Cherpes TL

25-7349 was used in Flow cytometry/Cell sorting to compare the ability of brefeldin A and monensin to concurrently trap 4 cytokines within peripheral blood mononuclear cells.

Wed Oct 31 00:00:00 EDT 2012

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

25-7349 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

25-7349 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine.

Virology

Lai L,Vödrös D,Kozlowski PA,Montefiori DC,Wilson RL,Akerstrom VL,Chennareddi L,Yu T,Kannanganat S,Ofielu L,Villinger F,Wyatt LS,Moss B,Amara RR,Robinson HL

25734982 was used in flow cytometry to assess the effects of using GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus-89.6 vaccine

Wed Dec 05 00:00:00 EST 2007

Bioinformatics

Protein Aliases: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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Function(s)

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

Process(es)

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TNF alpha Monoclonal Antibody (MAb11), PE-Cyanine7, eBioscience™

TNF alpha Antibody (25-7349-82) in Flow

Product Details

25-7349-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

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Published species

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