Invitrogen

TNF alpha Monoclonal Antibody (MAb11), PE-eFluor™ 610, eBioscience™

21 Published Figures
19 References
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

61-7349-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAb11

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574668

Product Specific Information

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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Function(s)
protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity positive regulation of cytokine production positive regulation of protein phosphorylation chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin glucose metabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response humoral immune response I-kappaB kinase/NF-kappaB signaling JNK cascade extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to virus response to salt stress positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity regulation of tumor necrosis factor-mediated signaling pathway negative regulation of lipid storage extracellular matrix organization osteoclast differentiation sequestering of triglyceride cortical actin cytoskeleton organization positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion negative regulation of myosin-light-chain-phosphatase activity positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import embryonic digestive tract development positive regulation of smooth muscle cell proliferation positive regulation of cytokine secretion positive regulation of phagocytosis regulation of insulin secretion defense response to Gram-positive bacterium leukocyte tethering or rolling negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound death-inducing signaling complex assembly positive regulation of mononuclear cell migration positive regulation of podosome assembly establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of NIK/NF-kappaB signaling positive regulation of superoxide dismutase activity regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell positive regulation of protein localization to cell surface positive regulation of ceramide biosynthetic process positive regulation of blood microparticle formation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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