Invitrogen

TRA-1-60 Monoclonal Antibody (TRA-1-60), DyLight™ 488

6 Published Figures

13 References

View all (11) TRA-1-60 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (6)

TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

TRA-1-60 Antibody (MA1-023-D488X) in ICC/IF

Imunofluorescent analysis of TRA-1-60 (green) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with DyLight 488-conjugated TRA-1-60 monoclonal antibody (left panels, Product # MA1-023-D488X) or DyLight 488-conjugated Mouse IgM isotype control antibody (top right panel, Product # MA1-194-D488), at a dilution of 1:250 and incubated for 1 hour at room temperature. N... View More

TRA-1-60 Antibody in Flow Cytometry (Flow)

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Product Details

MA1-023-D488X

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:250

View 1 publication 1 publication

Flow Cytometry (Flow)

1:100

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TRA-1-60

Immunogen

Human embryonal carcinoma cell line 2102Ep

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with 1mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536700

Product Specific Information

MA1-023-D488X has successfully been used in immunofluorescence assays with human samples.

Target Information

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

MA1-023-D488X was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 EDT 2022

Generation of an induced pluripotent stem cell line (TRNDi031-A) from a patient with Alagille syndrome type 1 carrying a heterozygous p. C312X (c. 936 T > A) mutation in JAGGED-1.

Stem cell research

Brooks BM,Pradhan M,Cheng YS,Gorshkov K,Farkhondeh A,Chen CZ,Beers J,Liu C,Baumgaertel K,Rodems S,Zheng W

MA1-023-D488X was used in Flow Cytometry to develop a human iPSC line to study the disease pathophysiology and develop therapeutics to treat patients with Alagille syndrome.

Thu Jul 01 00:00:00 EDT 2021

An induced pluripotent stem cell line (NCATS-CL9075) from a patient carrying compound heterozygote mutations, p.R390P and p.L318P, in the NGLY1 gene.

Stem cell research

Pradhan M,Farkhondeh A,Cheng YS,Xu M,Beers J,Zou J,Liu C,Might M,Rodems S,Baumgärtel K,Zheng W

MA1-023-D488X was used in Flow Cytometry to show that this cell-based iPSC disease model provides a resource to study disease pathophysiology and to develop a cell-based disease model for drug development for NGLY1 patients.

Thu Jul 01 00:00:00 EDT 2021

An induced pluripotent stem cell line (TRNDi001-D) from a Niemann-Pick disease type C1 (NPC1) patient carrying a homozygous p. I1061T (c. 3182T>C) mutation in the NPC1 gene.

Stem cell research

Li R,Pradhan M,Xu M,Roeder A,Beers J,Zou J,Liu C,Porter FD,Zheng W

MA1-023-D488X was used in Flow cytometry/Cell sorting to show that this NPC1 iPSC line offers a useful resource for disease modeling and drug development.

Wed Apr 01 00:00:00 EDT 2020

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

MA1-023-D488X was used in Flow cytometry/Cell sorting to confirm the authenticity of established iPSC lines.

Tue Dec 01 00:00:00 EST 2020

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

MA1-023-D488X was used in Flow cytometry/Cell sorting to confirm the authenticity of established iPSC lines.

Tue Dec 01 00:00:00 EST 2020

Generation of an induced pluripotent stem cell line (TRNDi004-I) from a Niemann-Pick disease type B patient carrying a heterozygous mutation of p.L43_A44delLA in the SMPD1 gene.

Stem cell research

Baskfield A,Li R,Beers J,Zou J,Liu C,Zheng W

MA1-023-D488X was used in Flow cytometry/Cell sorting to show that this iPSC line offers a useful resource to study the disease pathophysiology and as a cell-based model for drug development to treat NPB.

Wed May 01 00:00:00 EDT 2019

An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p.Glu153Lys in the NAGLU gene.

Stem cell research

Huang W,Xu M,Li R,Baskfield A,Kouznetsova J,Beers J,Zou J,Liu C,Zheng W

Published figure using TRA-1-60 monoclonal antibody (Product # MA1-023-D488X) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.

Stem cell research

Hong J,Xu M,Li R,Cheng YS,Kouznetsova J,Beers J,Liu C,Zou J,Zheng W

MA1-023-D488X was used in Flow cytometry/Cell sorting to document a human induced pluripotent stem cell (iPSC) line generated from dermal fibroblasts of a patient with Hunter syndrome containing a hemizygous mutation of a 1 bp insertion at nucleotide 208 in exon 2 of the IDS gene.

Wed May 01 00:00:00 EDT 2019

Generation of an induced pluripotent stem cell line (TRNDi002-B) from a patient carrying compound heterozygous p.Q208X and p.G310G mutations in the NGLY1 gene.

Stem cell research

Li R,Pradhan M,Xu M,Baskfield A,Farkhondeh A,Cheng YS,Beers J,Zou J,Liu C,Might M,Rodems S,Zheng W

MA1-023-D488X was used in Flow cytometry/Cell sorting to determine the disease phenotype of NGLY1 deficiency in patient cells.

Tue Jan 01 00:00:00 EST 2019

An induced pluripotent stem cell line (TRNDi009-C) from a Niemann-Pick disease type A patient carrying a heterozygous p.L302P (c.905 T>C) mutation in the SMPD1 gene.

Stem cell research

Baskfield A,Li R,Beers J,Zou J,Liu C,Zheng W

MA1-023-D488X was used in Flow cytometry/Cell sorting to study the disease pathophysiology and as a cell-based model for drug development to treat NPA.

Mon Jul 01 00:00:00 EDT 2019

A human induced pluripotent stem cell line (TRNDi007-B) from an infantile onset Pompe patient carrying p.R854X mutation in the GAA gene.

Stem cell research

Cheng YS,Li R,Baskfield A,Beers J,Zou J,Liu C,Zheng W

MA1-023-D488X was used in Flow cytometry/Cell sorting to develop a novel human induced pluripotent stem cell from a patient with Pompe disease (carrying the p.R854X mutaton in the GAA gene).

Wed May 01 00:00:00 EDT 2019

Generation of two induced pluripotent stem cell lines (NHLBIi001-A and NHLBIi001-B) from a healthy Caucasian female volunteer with normal cardiac function.

Stem cell research

Patterson K,Beers J,Linask KL,Lin Y,Hassanzadeh S,Sack MN,Zou J

MA1-023-D488X was used in Flow cytometry/Cell sorting to develop two wild-type human-derived induced pluripotent cell lines from a PBMC sample of a patient with normal cardiac function.

Sun Dec 01 00:00:00 EST 2019

PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons.

Journal of cellular physiology

Bonaventura G,Iemmolo R,D'Amico AG,La Cognata V,Costanzo E,Zappia M,D'Agata V,Conforti FL,Aronica E,Cavallaro S

Published figure using TRA-1-60 monoclonal antibody (Product # MA1-023-D488X) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: TRA160; tra60

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TRA-1-60 Monoclonal Antibody (TRA-1-60), DyLight™ 488

TRA-1-60 Antibody (MA1-023-D488X) in ICC/IF

Product Details

MA1-023-D488X

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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