Invitrogen

TRA-1-60 Monoclonal Antibody (TRA-1-60), PE

2 Published Figures

6 References

View all (11) TRA-1-60 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

TRA-1-60 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

TRA-1-60 Antibody (MA1-023-PE) in Flow

Flow cytometry analysis of TRA-1-60 in NCCIT (red filled histogram) and HeLa cells (red unfilled histogram) compared to unstained cells (blue histogram). Positive staining is observed on NCCIT cells when compared to no antibody control (middle panel) and to TRA-1-60-negative HeLa cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature, and incubated with a PE-conjugated TRA-1-60 monoclonal antibody (Product # MA1-023-PE) in 2% BSA-PBS at a dil... View More

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Product Details

MA1-023-PE

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:500

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TRA-1-60

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536704

Product Specific Information

MA1-023-PE has been successfully used in ICC/IF and flow cytometry applications on human samples.

Target Information

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Retinal ganglion cell-specific genetic regulation in primary open-angle glaucoma.

Cell genomics

Daniszewski M,Senabouth A,Liang HH,Han X,Lidgerwood GE,Hernández D,Sivakumaran P,Clarke JE,Lim SY,Lees JG,Rooney L,Gulluyan L,Souzeau E,Graham SL,Chan CL,Nguyen U,Farbehi N,Gnanasambandapillai V,McCloy RA,Clarke L,Kearns LS,Mackey DA,Craig JE,MacGregor S,Powell JE,Pébay A,Hewitt AW

MA1-023-PE was used in flow cytometry to assess the transcriptomic profile of disease-specific cell populations, fibroblasts from patients with primary open-angle glaucoma (POAG) were reprogrammed into induced pluripotent stem cells (iPSCs) before being differentiated into retinal organoids and compared with those from healthy individuals.

Wed Jun 08 00:00:00 EDT 2022

Transcriptomic and proteomic retinal pigment epithelium signatures of age-related macular degeneration.

Nature communications

Senabouth A,Daniszewski M,Lidgerwood GE,Liang HH,Hernández D,Mirzaei M,Keenan SN,Zhang R,Han X,Neavin D,Rooney L,Lopez Sanchez MIG,Gulluyan L,Paulo JA,Clarke L,Kearns LS,Gnanasambandapillai V,Chan CL,Nguyen U,Steinmann AM,McCloy RA,Farbehi N,Gupta VK,Mackey DA,Bylsma G,Verma N,MacGregor S,Watt MJ,Guymer RH,Powell JE,Hewitt AW,Pébay A

MA1-023-PE was used in Immunocytochemistry to uncover important differences in retinal pigment epithelium homeostasis associated with geographic atrophy.

Tue Jul 26 00:00:00 EDT 2022

Generation of a gene-corrected human isogenic iPSC line from an Alzheimer's disease iPSC line carrying the London mutation in APP (V717I).

Stem cell research

Hernández D,Morgan Schlicht S,Daniszewski M,Karch CM,Goate AM,Pébay A

MA1-023-PE was used in Immunocytochemistry-immunoflourescence to report the genome-editing of an existing iPSC line carrying the London mutation in APP (V717I) into an iPSC line in which the pathogenic mutation was corrected.

Sat May 01 00:00:00 EDT 2021

NAMPT/SIRT2-mediated inhibition of the p53-p21 signaling pathway is indispensable for maintenance and hematopoietic differentiation of human iPS cells.

Stem cell research & therapy

Xu Y,Nasri M,Dannenmann B,Mir P,Zahabi A,Welte K,Morishima T,Skokowa J

MA1-023-PE was used in Flow Cytometry to the effects of specific inhibitors of Nicotinamide phosphoribosyltransferase (NAMPT) or nicotinamide adenine dinucleotide (NAD+)-dependent sirtuins (SIRTs) on the pluripotency, proliferation, survival, and hematopoietic differentiation of human iPS cells.

Fri Feb 05 00:00:00 EST 2021

NAMPT/SIRT2-mediated inhibition of the p53-p21 signaling pathway is indispensable for maintenance and hematopoietic differentiation of human iPS cells.

Stem cell research & therapy

Xu Y,Nasri M,Dannenmann B,Mir P,Zahabi A,Welte K,Morishima T,Skokowa J

Fri Feb 05 00:00:00 EST 2021

Single cell eQTL analysis identifies cell type-specific genetic control of gene expression in fibroblasts and reprogrammed induced pluripotent stem cells.

Genome biology

Neavin D,Nguyen Q,Daniszewski MS,Liang HH,Chiu HS,Wee YK,Senabouth A,Lukowski SW,Crombie DE,Lidgerwood GE,Hernández D,Vickers JC,Cook AL,Palpant NJ,Pébay A,Hewitt AW,Powell JE

MA1-023-PE was used in microarray to demonstrate that the majority of eQTLs detected in fibroblasts are specific to an individual cell subtype.

Fri Mar 05 00:00:00 EST 2021

Quantification of Etoposide Hypersensitivity: A Sensitive, Functional Method for Assessing Pluripotent Stem Cell Quality.

Stem cells translational medicine

Secreto FJ,Li X,Smith AJ,Bruinsma ES,Perales-Clemente E,Oommen S,Hawse G,Hrstka SCL,Arendt BK,Brandt EB,Wigle DA,Nelson TJ

Published figure using TRA-1-60 monoclonal antibody (Product # MA1-023-PE) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: TRA160; tra60

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