Invitrogen

TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), Biotin, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

18 References

View all (11) TRA-1-60 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TRA-1-60 (Podocalyxin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 17

TRA-1-60 (Podocalyxin) Antibody (13-8863-82) in Flow

Staining of 2102Ep human embryonal carcinoma cell line with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Human TRA-1-60 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

TRA-1-60 (Podocalyxin) Antibody in Immunocytochemistry (ICC/IF)

TRA-1-60 (Podocalyxin) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

13-8863-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 13 publications 13 publications

Flow Cytometry (Flow)

0.25 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TRA-1-60

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_891594

Product Specific Information

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Attenuating iPSC reprogramming stress with dominant-negative BET peptides.

iScience

Hossain ME,Cevallos RR,Zhang R,Hu K

13-8863-82 was used in flow cytometry experiments to develop the concept of reprogramming stress, affording an alternative avenue to understanding and improving iPSC reprogramming.

Fri Jan 20 00:00:00 EST 2023

Multisite studies for validation and improvement of a highly efficient culture assay for detection of undifferentiated human pluripotent stem cells intermingled in cell therapy products.

Cytotherapy

Watanabe T,Yasuda S,Kusakawa S,Kuroda T,Futamura M,Ogawa M,Mochizuki H,Kikkawa E,Furukawa H,Nagaoka M,Sato Y

13-8863 was used in Immunocytochemistry-immunoflourescence to validate the detection sensitivity of HEC assay and then investigate the feasibility of magnetic activated cell sorting (MACS) to improve the sensitivity.

Mon Feb 01 00:00:00 EST 2021

Establishment of a novel human iPSC line (YCMi003-A) from a patient with dilated cardiomyopathy carrying genetic variant LMNA p.Asp364His.

Stem cell research

Oh J,Lee SH,Choi J,Choi JR,Kim S,Cha YJ,Choi HK,Won D,Yoon HG,Park SW,Kang SM,Lee ST,Lee SH

13-8863-82 was used in Immunocytochemistry-immunoflourescence to generate a novel human induced pluripotent stem cell(iPSC) lines YCMi003-A from a patient with dilated cardiomyopathy associated with genetic variant LMNA c.1090G > C; p.Asp364His.

Fri Oct 01 00:00:00 EDT 2021

Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing.

Science advances

Xing QR,El Farran CA,Gautam P,Chuah YS,Warrier T,Toh CD,Kang NY,Sugii S,Chang YT,Xu J,Collins JJ,Daley GQ,Li H,Zhang LF,Loh YH

13-8863 was used in Immunohistochemistry to illuminate the multitude of diverse routes transversed by individual reprogramming cells and presents an integrative roadmap for identifying the mechanistic part list of the reprogramming machinery.

Tue Sep 01 00:00:00 EDT 2020

Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells.

Cell

Nakanishi M,Mitchell RR,Benoit YD,Orlando L,Reid JC,Shimada K,Davidson KC,Shapovalova Z,Collins TJ,Nagy A,Bhatia M

13-8863 was used in Immunocytochemistry-immunoflourescence to identify human pluripotent founder cells (hPFCs) that initiate, as well as preserve and establish, pluripotent stem cell (PSC) cultures.

Thu May 02 00:00:00 EDT 2019

Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming.

Nature chemical biology

Ebrahimi A,Sevinç K,Gürhan Sevinç G,Cribbs AP,Philpott M,Uyulur F,Morova T,Dunford JE,Göklemez S,Arı Ş,Oppermann U,Önder TT

13-8863 was used in Immunocytochemistry to show that CBP/EP300 bromodomains sustain cell type specific gene expression and maintain cell identity.

Wed May 01 00:00:00 EDT 2019

Transient activation of the UPR^ER is an essential step in the acquisition of pluripotency during reprogramming.

Science advances

Simic MS,Moehle EA,Schinzel RT,Lorbeer FK,Halloran JJ,Heydari K,Sanchez M,Jullié D,Hockemeyer D,Dillin A

13-8863 was used in Immunocytochemistry to find that the unfolded protein response of the endoplasmic reticulum (UPRER), the mitochondrial UPR, and the heat shock response, which ensure proteome quality during stress, are activated during reprogramming.

Mon Apr 01 00:00:00 EDT 2019

LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.

Nature cell biology

Tsanov KM,Pearson DS,Wu Z,Han A,Triboulet R,Seligson MT,Powers JT,Osborne JK,Kane S,Gygi SP,Gregory RI,Daley GQ

13-8863 was used in Immunocytochemistry to show that LIN28 is phosphorylated by MAPK/ERK in pluripotent stem cells.

Sun Jan 01 00:00:00 EST 2017

Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).

Stem cell reviews and reports

Suchorska WM,Augustyniak E,Richter M,Trzeciak T

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes.

Stem cell reports

Oleksiewicz U,Gładych M,Raman AT,Heyn H,Mereu E,Chlebanowska P,Andrzejewska A,Sozańska B,Samant N,Fąk K,Auguścik P,Kosiński M,Wróblewska JP,Tomczak K,Kulcenty K,Płoski R,Biecek P,Esteller M,Shah PK,Rai K,Wiznerowicz M

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Flow Cytometry

Tue Dec 12 00:00:00 EST 2017

The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Scientific reports

Debowski K,Drummer C,Lentes J,Cors M,Dressel R,Lingner T,Salinas-Riester G,Fuchs S,Sasaki E,Behr R

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Immunocytochemistry

Thu Jul 07 00:00:00 EDT 2016

Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations.

PloS one

Momcilovic O,Sivapatham R,Oron TR,Meyer M,Mooney S,Rao MS,Zeng X

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Immunofluorescence

Fri Jul 07 00:00:00 EDT 2017

Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.

PloS one

Debowski K,Warthemann R,Lentes J,Salinas-Riester G,Dressel R,Langenstroth D,Gromoll J,Sasaki E,Behr R

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Immunofluorescence

Tue Feb 23 00:00:00 EST 2016

High-risk human papillomavirus E6 protein promotes reprogramming of Fanconi anemia patient cells through repression of p53 but does not allow for sustained growth of induced pluripotent stem cells.

Journal of virology

Chlon TM,Hoskins EE,Mayhew CN,Wikenheiser-Brokamp KA,Davies SM,Mehta P,Myers KC,Wells JM,Wells SI

13-8863 was used in Immunofluorescence to utilize the oncogenes of high-risk human papillomavirus 16 (HPV16) to overcome the resistance of FA patient cells to reprogramming.

Wed Oct 01 00:00:00 EDT 2014

PRMT5 is required for human embryonic stem cell proliferation but not pluripotency.

Stem cell reviews and reports

Gkountela S,Li Z,Chin CJ,Lee SA,Clark AT

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Chromatin-modifying enzymes as modulators of reprogramming.

Nature

Onder TT,Kara N,Cherry A,Sinha AU,Zhu N,Bernt KM,Cahan P,Marcarci BO,Unternaehrer J,Gupta PB,Lander ES,Armstrong SA,Daley GQ

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Immunohistochemistry

Sun Mar 04 00:00:00 EST 2012

JD induced pluripotent stem cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia.

Hepatology (Baltimore, Md.)

Cayo MA,Cai J,DeLaForest A,Noto FK,Nagaoka M,Clark BS,Collery RF,Si-Tayeb K,Duncan SA

Published figure using TRA-1-60 (Podocalyxin) monoclonal antibody (Product # 13-8863-82) in Immunocytochemistry

Sat Dec 01 00:00:00 EST 2012

Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity.

Nature cell biology

Kuo TC,Chen CT,Baron D,Onder TT,Loewer S,Almeida S,Weismann CM,Xu P,Houghton JM,Gao FB,Daley GQ,Doxsey S

13-8863 was used in Immunofluorescence to investigate the fate of midbodies.

Sun Sep 11 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: TRA160; tra60

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TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), Biotin, eBioscience™

TRA-1-60 (Podocalyxin) Antibody (13-8863-82) in Flow

Product Details

13-8863-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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