GenScript

V5 Tag Monoclonal Antibody (4C12E11), THE™

V5 Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

V5 Tag Antibody (A01724-100) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) and 5 fusion gene transfected CHO cells (b) using THE™ 5 Antibody, mAb, Mouse (GenScript, A01724). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

V5 Tag Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

A01724-100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

Immunocytochemistry (ICC/IF)

1-3 µg/mL

Flow Cytometry (Flow)

1-3 µg 1 x 10^6 cells

ELISA (ELISA)

0.05-0.1 µg/mL

Immunoprecipitation (IP)

1-5 µg/mL

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4C12E11

Immunogen

V5 tag peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.THE™ V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Soluble RARRES1 induces podocyte apoptosis to promote glomerular disease progression.

The Journal of clinical investigation

Chen A,Feng Y,Lai H,Ju W,Li Z,Li Y,Wang A,Hong Q,Zhong F,Wei C,Fu J,Guan T,Liu B,Kretzler M,Lee K,He JC

Using the Nephrotic Syndrome Study Network Consortium data set and other publicly available transcriptomic data sets, we identified retinoic acid receptor responder protein 1 (RARRES1) as a gene whose expression positively correlated with renal function decline in human glomerular disease. The glomerular expression of RARRES1, which is largely restricted to podocytes, increased in focal segmental glomerulosclerosis (FSGS) and diabetic kidney disease (DKD). TNF-α was a potent inducer of RAR

Thu Oct 01 00:00:00 EDT 2020

Mithramycin suppresses DNA damage repair via targeting androgen receptor in prostate cancer.

Cancer letters

Wang S,Gilbreath C,Kollipara RK,Sonavane R,Huo X,Yenerall P,Das A,Ma S,Raj GV,Kittler R

The dependency of prostate cancer (PCa) growth on androgen receptor (AR) signaling has been harnessed to develop first-line therapies for high-risk localized and metastatic PCa treatment. However, the occurrence of aberrant expression, mutated or splice variants of AR confers resistance to androgen ablation therapy (ADT), radiotherapy or chemotherapy in AR-positive PCa. Therapeutic strategies that effectively inhibit the expression and/or transcriptional activity of full-length AR, mutated AR an

Mon Sep 28 00:00:00 EDT 2020

RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.

Cell chemical biology

Yenerall P,Das AK,Wang S,Kollipara RK,Li LS,Villalobos P,Flaming J,Lin YF,Huffman K,Timmons BC,Gilbreath C,Sonavane R,Kinch LN,Rodriguez-Canales J,Moran C,Behrens C,Hirasawa M,Takata T,Murakami R,Iwanaga K,Chen BPC,Grishin NV,Raj GV,Wistuba II,Minna JD,Kittler R

Thu Jan 16 00:00:00 EST 2020

SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts.

Journal of the American Society of Nephrology : JASN

Wei C,Banu K,Garzon F,Basgen JM,Philippe N,Yi Z,Liu R,Choudhuri J,Fribourg M,Liu T,Cumpelik A,Wong J,Khan M,Das B,Keung K,Salem F,Campbell KN,Kaufman L,Cravedi P,Zhang W,O'Connell PJ,He JC,Murphy B,Menon MC

We previously showed that the presence of a CKD-associated locus in SHROOM3 in a donor kidney results in increased expression of SHROOM3 (an F-actin-binding protein important for epithelial morphogenesis, via rho-kinase [ROCK] binding); this facilitates TGF-b signaling and allograft fibrosis. However, other evidence suggests Shroom3 may have a protective role in glomerular development.,We used human data, Shroom3 knockdown podocytes, and inducible shRNA-mediated knockdown mice to study the role

Thu Nov 01 00:00:00 EDT 2018

The synergistic effect of combination temozolomide and chloroquine treatment is dependent on autophagy formation and p53 status in glioma cells.

Cancer letters

Lee SW,Kim HK,Lee NH,Yi HY,Kim HS,Hong SH,Hong YK,Joe YA

Temozolomide (TMZ) is an alkylating agent used for the treatment of glioblastoma. The late autophagy inhibitor chloroquine (CQ) inhibits glioblastoma tumors in a p53-independent and p53-dependent manner. We addressed a possible beneficial effect of combination treatment with TMZ and CQ by examining the molecular and cellular mechanism of co-treatment. Combination treatment of U87 cell (wild type p53) with TMZ and CQ synergistically reduced cell proliferation and enhanced apoptosis with increased

Fri May 01 00:00:00 EDT 2015

Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity.

Scientific reports

Kim HS,Li A,Ahn S,Song H,Zhang W

Glioblastoma (GBM), the most common type of primary malignant brain tumors harboring a subpopulation of stem-like cells (GSCs), is a fast-growing and often fatal tumor. Signal Transducer and Activator of Transcription 3 (STAT3) is one of the major signaling pathways in GSCs maintenance but the molecular mechanisms underlying STAT3 deregulation in GSCs are poorly defined. Here, we demonstrate that Inositol Polyphosphate-5-Phosphatase F (INPP5F), one of the polyphosphoinositide phosphatases, is di

Fri Dec 05 00:00:00 EST 2014

Bioinformatics

Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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