Invitrogen

V5 Tag Monoclonal Antibody (E10/V4RR), HRP

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

8 References

View all (76) V5 Tag antibodies

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V5 Tag Antibody (MA5-15253-HRP) in WB

Western blot was performed using V5 Tag Monoclonal Antibody (E10/V4RR), HRP (Product # MA5-15253-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), V5-H3-His, 50 µg (Lane 3), Myc-p65-V5, 50 µg (Lane 4), Myc-p65-V5, 25 µg (Lane 5), Myc-p65-V5, 12.5 µg (Lane 6), Myc-p65-V5, 6.26 µg (Lane 7), p65-HA, 50 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX)... View More

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Product Details

MA5-15253-HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E10/V4RR

Immunogen

GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.07% Kathon

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537645

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PLK4 deubiquitination by Spata2-CYLD suppresses NEK7-mediated NLRP3 inflammasome activation at the centrosome.

The EMBO journal

Yang XD,Li W,Zhang S,Wu D,Jiang X,Tan R,Niu X,Wang Q,Wu X,Liu Z,Chen LF,Qin J,Su B

MA5-15253-HRP was used in Western Blotting to show that NLRP3 inflammasome activation is suppressed by a centrosomal protein Spata2.

Wed Jan 15 00:00:00 EST 2020

The rice NLR pair Pikp-1/Pikp-2 initiates cell death through receptor cooperation rather than negative regulation.

PloS one

Zdrzałek R,Kamoun S,Terauchi R,Saitoh H,Banfield MJ

Published figure using V5 Tag monoclonal antibody (Product # MA5-15253-HRP) in Western Blot

Wed Oct 28 00:00:00 EDT 2020

Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility.

Cell

Hwang JY,Mannowetz N,Zhang Y,Everley RA,Gygi SP,Bewersdorf J,Lishko PV,Chung JJ

MA5-15253-HRP was used in Western Blot to demonstrate that EFCAB9, in complex with the CatSper subunit, CATSPERζ, is essential for pH-dependent and Ca2+-sensitive activation of the CatSper channel.

Thu May 30 00:00:00 EDT 2019

VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells.

eLife

Mohan C,Kim LM,Hollar N,Li T,Paulissen E,Leung CT,Luk E

Published figure using V5 Tag monoclonal antibody (Product # MA5-15253-HRP) in Western Blot

Thu Aug 09 00:00:00 EDT 2018

Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or β Cell Metabolism in the Absence of Overnutrition.

Cell reports

Peterson BS,Campbell JE,Ilkayeva O,Grimsrud PA,Hirschey MD,Newgard CB

Published figure using V5 Tag monoclonal antibody (Product # MA5-15253-HRP) in Western Blot

Tue Jul 03 00:00:00 EDT 2018

Mutations in Fibronectin Cause a Subtype of Spondylometaphyseal Dysplasia with "Corner Fractures".

American journal of human genetics

Lee CS,Fu H,Baratang N,Rousseau J,Kumra H,Sutton VR,Niceta M,Ciolfi A,Yamamoto G,Bertola D,Marcelis CL,Lugtenberg D,Bartuli A,Kim C,Hoover-Fong J,Sobreira N,Pauli R,Bacino C,Krakow D,Parboosingh J,Yap P,Kariminejad A,McDonald MT,Aracena MI,Lausch E,Unger S,Superti-Furga A,Lu JT,Cohn DH,Tartaglia M,Lee BH,Reinhardt DP,Campeau PM

MA5-15253-HRP was used in Western Blotting to study the fibronectin mutations responsible for defective fibronectin secretion in spondylometaphyseal dysplasias.

Thu Nov 02 00:00:00 EDT 2017

In vitro metabolism of exemestane by hepatic cytochrome P450s: impact of nonsynonymous polymorphisms on formation of the active metabolite 17β-dihydroexemestane.

Pharmacology research & perspectives

Peterson A,Xia Z,Chen G,Lazarus P

MA5-15253-HRP was used in Western Blotting to show that functional polymorphisms in exemestane-metabolizing CYP450s contribute to variability in patient outcomes by mediating exemestane exposure and its active metabolite, 17Β-DHE.

Thu Jun 01 00:00:00 EDT 2017

Functional Comparison of Mx1 from Two Different Mouse Species Reveals the Involvement of Loop L4 in the Antiviral Activity against Influenza A Viruses.

Journal of virology

Verhelst J,Spitaels J,Nürnberger C,De Vlieger D,Ysenbaert T,Staeheli P,Fiers W,Saelens X

Published figure using V5 Tag monoclonal antibody (Product # MA5-15253-HRP) in Western Blot

Sun Nov 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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V5 Tag Monoclonal Antibody (E10/V4RR), HRP

V5 Tag Antibody (MA5-15253-HRP) in WB

Product Details

MA5-15253-HRP

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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