Invitrogen

Vimentin Monoclonal Antibody (V9), FITC, eBioscience™

Vimentin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Vimentin Antibody (11-9897-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized C6 cells using 1 µg/mL of Mouse IgG1 Isotype Control FITC (Product # 11-4714-42) (left) or 1 µg/mL of Anti-Vimentin FITC (right). Nuclei are counterstained with DAPI.

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-9897-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 6 publications 6 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597000

Product Specific Information

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.

This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.

Applications Reported: This V9 antibody has been reported for use in immunocytochemistry and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).

Applications Tested: This V9 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cells.

iScience

Lopez-Cavestany M,Hahn SB,Hope JM,Reckhorn NT,Greenlee JD,Schwager SC,VanderBurgh JA,Reinhart-King CA,King MR

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Fri Apr 21 00:00:00 EDT 2023

Pericardial malignant mesothelioma diagnosed in a dog by immunocytochemistry of the pericardial fluid: a case report.

BMC veterinary research

Choi EW

11-9897-82 was used in Immunocytochemistry to show that E-cadherin and calretinin can be used for the differential diagnosis of reactive mesothelial cells, malignant mesothelioma, and adenocarcinoma in dogs.

Fri Jul 21 00:00:00 EDT 2023

Aspirin increases the efficacy of gemcitabine in pancreatic cancer by modulating the PI3K/AKT/mTOR signaling pathway and reversing epithelial‑mesenchymal transition.

Oncology letters

Zhou H,Yun X,Shu Y,Xu K

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Wed Mar 01 00:00:00 EST 2023

Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.

Theranostics

Lee YA,Lek CCJ,Rong G,Wu Z,Shathishwaran S,Lee JHJ,Tam WL,Wuestefeld T,Park SJ,Jung S,Kim B,Kang NY,Chang YT

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Mon Mar 20 00:00:00 EDT 2023

Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.

Theranostics

Lee YA,Lek CCJ,Rong G,Wu Z,Shathishwaran S,Lee JHJ,Tam WL,Wuestefeld T,Park SJ,Jung S,Kim B,Kang NY,Chang YT

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Mon Mar 20 00:00:00 EDT 2023

Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity.

Journal of nanobiotechnology

Zanjanchi P,Asghari SM,Mohabatkar H,Shourian M,Shafiee Ardestani M

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Tue Jan 04 00:00:00 EST 2022

Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform.

Biofabrication

Jung O,Tung YT,Sim E,Chen YC,Lee E,Ferrer M,Song MJ

11-9897-82 was used in Flow Cytometry to develop two novel vascularized, LRT multi-chip models for the alveoli and the small airway.

Mon Feb 14 00:00:00 EST 2022

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.

Histochemistry and cell biology

Giotakis AI,Dudas J,Glueckert R,Dejaco D,Ingruber J,Fleischer F,Innerhofer V,Pinggera L,Bektic-Tadic L,Gabriel SAM,Riechelmann H

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Mon Mar 01 00:00:00 EST 2021

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.

Histochemistry and cell biology

Giotakis AI,Dudas J,Glueckert R,Dejaco D,Ingruber J,Fleischer F,Innerhofer V,Pinggera L,Bektic-Tadic L,Gabriel SAM,Riechelmann H

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Mon Mar 01 00:00:00 EST 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Western Blot

Thu Apr 01 00:00:00 EDT 2021

Defining tumor-associated vascular heterogeneity in pediatric high-grade and diffuse midline gliomas.

Acta neuropathologica communications

Wei X,Meel MH,Breur M,Bugiani M,Hulleman E,Phoenix TN

11-9897-82 was used in Immunohistochemistry (Paraffin) to characterise and directly compare tumor-associated vasculature in cortical pediatric high-grade gliomas and diffuse midline gliomas.

Mon Aug 23 00:00:00 EDT 2021

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Wed May 06 00:00:00 EDT 2020

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Wed May 06 00:00:00 EDT 2020

GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells.

Stem cell research & therapy

McGrath M,Tam E,Sladkova M,AlManaie A,Zimmer M,de Peppo GM

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunocytochemistry

Fri Jan 11 00:00:00 EST 2019

Levels of hepatic Th17 cells and regulatory T cells upregulated by hepatic stellate cells in advanced HBV-related liver fibrosis.

Journal of translational medicine

Li X,Su Y,Hua X,Xie C,Liu J,Huang Y,Zhou L,Zhang M,Li X,Gao Z

Published figure using Vimentin monoclonal antibody (Product # 11-9897-82) in Immunofluorescence

Tue Apr 11 00:00:00 EDT 2017

Chronic macrophage migration inhibitory factor exposure induces mesenchymal epithelial transition and promotes gastric and colon cancers.

PloS one

Morris KT,Nofchissey RA,Pinchuk IV,Beswick EJ

11-9897 was used in Flow cytometry/Cell sorting to study how the MIF promotes mesenchymal epithelial transition, cell transformation and tumorigenesis in GI cancers, and thus may be an important link between chronic inflammation and tumorigenesis.

Tue Jan 20 00:00:00 EST 2015

Expression of the progenitor marker NG2/CSPG4 predicts poor survival and resistance to ionising radiation in glioblastoma.

Acta neuropathologica

Svendsen A,Verhoeff JJ,Immervoll H,Brøgger JC,Kmiecik J,Poli A,Netland IA,Prestegarden L,Planagumà J,Torsvik A,Kjersem AB,Sakariassen PØ,Heggdal JI,Van Furth WR,Bjerkvig R,Lund-Johansen M,Enger PØ,Felsberg J,Brons NH,Tronstad KJ,Waha A,Chekenya M

11-9897 was used in Immunohistochemistry to investigate whether expression of neuron-glial-2 influences the survival of glioblastoma patients.

Sat Oct 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus

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Gene Aliases: CTRCT30; HEL113; VIM

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UniProt ID: (Human) P08670, (Dog) A0A8C0N8E3, (Rat) P31000

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Entrez Gene ID: (Chicken) 420519, (Human) 7431, (Dog) 477991, (Rat) 81818

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Function(s)

glycoprotein binding double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein binding protein C-terminus binding identical protein binding scaffold protein binding keratin filament binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

movement of cell or subcellular component positive regulation of gene expression negative regulation of neuron projection development astrocyte development viral process muscle filament sliding intermediate filament organization Bergmann glial cell differentiation SMAD protein signal transduction lens fiber cell development aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus intermediate filament-based process fat cell differentiation decidualization regulation of axonogenesis positive regulation of glial cell proliferation regulation of Schwann cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Vimentin Monoclonal Antibody (V9), FITC, eBioscience™

Vimentin Antibody (11-9897-82) in ICC/IF

Product Details

11-9897-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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