Invitrogen

c-MAF Monoclonal Antibody (sym0F1), eFluor™ 660, eBioscience™

FIGURE: 1 / 6

c-MAF Antibody (50-9855-82) in Flow

Intracellular staining of 3-day Th17-polarized mouse splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.06 µg of Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or 0.06 µg of Anti-Human/Mouse c-Maf eFluor® 660 (right) using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

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Product Details

50-9855-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fungi, Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

sym0F1

Immunogen

Full-length E. coli-produced c-Maf

View immunogen

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574388

Product Specific Information

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.

Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This sym0F1 antibody has been tested by intracellular staining followed by flow cytometric analysis of TCR-activated mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

Published figure using c-MAF monoclonal antibody (Product # 50-9855-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2023

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

Published figure using c-MAF monoclonal antibody (Product # 50-9855-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using c-MAF monoclonal antibody (Product # 50-9855-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Fungal sensing by dectin-1 directs the non-pathogenic polarization of T_H17 cells through balanced type I IFN responses in human DCs.

Nature immunology

Gringhuis SI,Kaptein TM,Remmerswaal EBM,Drewniak A,Wevers BA,Theelen B,D'Haens GRAM,Boekhout T,Geijtenbeek TBH

50-9855-82 was used in Flow Cytometry to show that dectin-1-mediated modulation of type I IFN responses allowed TGF-β activation and non-pathogenic TH17 cell development during fungal infections in humans.

Thu Dec 01 00:00:00 EST 2022

An immunoregulatory and tissue-residency program modulated by c-MAF in human T_H17 cells.

Nature immunology

Aschenbrenner D,Foglierini M,Jarrossay D,Hu D,Weiner HL,Kuchroo VK,Lanzavecchia A,Notarbartolo S,Sallusto F

50-9855 was used in Flow cytometry/Cell sorting to identify c-MAF as a relevant factor that drives two highly divergent post-activation fates of human TH17 cells.

Mon Oct 01 00:00:00 EDT 2018

C5aR1 regulates T follicular helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans.

JCI insight

Verghese DA,Chun N,Paz K,Fribourg M,Woodruff TM,Flynn R,Hu Y,Xiong H,Zhang W,Yi Z,Du J,Blazar BR,Heeger PS

Published figure using c-MAF monoclonal antibody (Product # 50-9855-82) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2018

Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence.

PLoS pathogens

Clement M,Marsden M,Stacey MA,Abdul-Karim J,Gimeno Brias S,Costa Bento D,Scurr MJ,Ghazal P,Weaver CT,Carlesso G,Clare S,Jones SA,Godkin A,Jones GW,Humphreys IR

50-9855 was used in Flow cytometry/Cell sorting to reveal that cytokine signalling events during initial infection profoundly influence virus chronicity.

Thu Dec 01 00:00:00 EST 2016

Human circulating PD-1+CXCR3-CXCR5+ memory Tfh cells are highly functional and correlate with broadly neutralizing HIV antibody responses.

Immunity

Locci M,Havenar-Daughton C,Landais E,Wu J,Kroenke MA,Arlehamn CL,Su LF,Cubas R,Davis MM,Sette A,Haddad EK,Poignard P,Crotty S

Thu Oct 17 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; c-Maf long form; c-maf proto-oncogene; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog

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Gene Aliases: 2810401A20Rik; A230108G15Rik; AW047063; AYGRP; c-MAF; CCA4; CTRCT21; MAF; Maf2

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UniProt ID: (Human) O75444, (Mouse) P54843

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Entrez Gene ID: (Human) 4094, (Mouse) 17132

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Function(s)

transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein homodimerization activity protein heterodimerization activity basic leucine zipper transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter cytokine production regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter regulation of chondrocyte differentiation positive regulation of transcription from RNA polymerase II promoter cell development inner ear development lens fiber cell differentiation lens development in camera-type eye transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of gene expression

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-MAF Monoclonal Antibody (sym0F1), eFluor™ 660, eBioscience™

c-MAF Antibody (50-9855-82) in Flow

Product Details

50-9855-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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