Invitrogen

iNOS Monoclonal Antibody (CXNFT), APC, eBioscience™

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Antibody Testing Data (1)
Published Figures (28)

FIGURE: 1 / 29

iNOS Antibody (17-5920-82) in Flow

Mouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS then surface stained with Anti-Mouse CD11b eFluor® 450 (Product # 48-0112-82) followed by fixation and permeabilization with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). The cells were then intracellularly stained with 0.03 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.03 µg of Anti-Mouse NOS2 APC (right). Total viable cells were used for analysis.

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Product Details

17-5920-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 42 publications 42 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CXNFT

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573244

Product Specific Information

Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.

Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This CXNFT antibody has been tested by intracellular staining and flow cytometric analysis of LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Zhang Y,Chen Y,Ding T,Zhang Y,Yang D,Zhao Y,Liu J,Ma B,Bianco A,Ge S,Li J

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Sat Jun 03 00:00:00 EDT 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

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Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

A druggable copper-signalling pathway that drives inflammation.

Nature

Solier S,Müller S,Cañeque T,Versini A,Mansart A,Sindikubwabo F,Baron L,Emam L,Gestraud P,Pantoș GD,Gandon V,Gaillet C,Wu TD,Dingli F,Loew D,Baulande S,Durand S,Sencio V,Robil C,Trottein F,Péricat D,Näser E,Cougoule C,Meunier E,Bègue AL,Salmon H,Manel N,Puisieux A,Watson S,Dawson MA,Servant N,Kroemer G,Annane D,Rodriguez R

17-5920-82 was used in Flow Cytometry to highlight the central role of copper as a regulator of cell plasticity and unveils a therapeutic strategy based on metabolic reprogramming and the control of epigenetic cell states.

Mon May 01 00:00:00 EDT 2023

IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondii.

Cell reports

Clark JT,Weizman OE,Aldridge DL,Shallberg LA,Eberhard J,Lanzar Z,Wasche D,Huck JD,Zhou T,Ring AM,Hunter CA

17-5920-82 was used in Flow cytometry/Cell sorting to investigate the role of interleukin-18 (IL-18) and its binding protein (IL-18BP) in controlling Toxoplasma gondii infection.

Tue Mar 28 00:00:00 EDT 2023

ROS-responsive polymer nanoparticles with enhanced loading of dexamethasone effectively modulate the lung injury microenvironment.

Acta biomaterialia

Muhammad W,Zhu J,Zhai Z,Xie J,Zhou J,Feng X,Feng B,Pan Q,Li S,Venkatesan R,Li P,Cao H,Gao C

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis.

Cell death & disease

Zhang X,Chen C,Ling C,Luo S,Xiong Z,Liu X,Liao C,Xie P,Liu Y,Zhang L,Chen Z,Liu Z,Tang J

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Mon Nov 07 00:00:00 EST 2022

Cutting Edge: Enhanced Antitumor Immunity in ST8Sia6 Knockout Mice.

Journal of immunology (Baltimore, Md. : 1950)

Friedman DJ,Kizerwetter M,Belmonte P,Rajcula M,Theodore K,Kim Lee HS,Shapiro MJ,Dong H,Shapiro VS

17-5920-82 was used in Flow cytometry/Cell sorting to examine the role of ST8Sia6 on immune cells in regulating antitumor immunity. ST8Sia6 knockout mice had an enhanced immune response to tumors.

Fri Apr 15 00:00:00 EDT 2022

Neuropeptide Y attenuates cardiac remodeling and deterioration of function following myocardial infarction.

Molecular therapy : the journal of the American Society of Gene Therapy

Qin YY,Huang XR,Zhang J,Wu W,Chen J,Wan S,Yu XY,Lan HY

17-5920-82 was used in Flow cytometry/Cell sorting to find that AMI mice lacking NPY developed more severe AMI than WT mice with worse cardiac dysfunction, progressive cardiac inflammation and fibrosis, and excessive apoptosis but impairing angiogenesis.

Wed Feb 02 00:00:00 EST 2022

Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells.

Immunity

Formaglio P,Alabdullah M,Siokis A,Handschuh J,Sauerland I,Fu Y,Krone A,Gintschel P,Stettin J,Heyde S,Mohr J,Philipsen L,Schröder A,Robert PA,Zhao G,Khailaie S,Dudeck A,Bertrand J,Späth GF,Kahlfuß S,Bousso P,Schraven B,Huehn J,Binder S,Meyer-Hermann M,Müller AJ

17-5920-82 was used in Flow cytometry/Cell sorting to conclude that NO fulfills two distinct functions for L. major containment: permitting direct killing and restricting the supply of proliferation-permissive host cells.

Tue Dec 14 00:00:00 EST 2021

Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells.

Immunity

Tue Dec 14 00:00:00 EST 2021

Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade.

Cancer cell

Srivastava S,Furlan SN,Jaeger-Ruckstuhl CA,Sarvothama M,Berger C,Smythe KS,Garrison SM,Specht JM,Lee SM,Amezquita RA,Voillet V,Muhunthan V,Yechan-Gunja S,Pillai SPS,Rader C,Houghton AM,Pierce RH,Gottardo R,Maloney DG,Riddell SR

17-5920-82 was used in Flow Cytometry to test strategies for enhancing efficacy of CAR-T cells targeting the tumor-associated antigen receptor tyrosine kinase-like orphan receptor 1 (ROR1) infiltrating tumors.

Mon Feb 08 00:00:00 EST 2021

Neutrophil-Macrophage Imbalance Drives the Development of Renal Scarring during Experimental Pyelonephritis.

Journal of the American Society of Nephrology : JASN

Ruiz-Rosado JD,Robledo-Avila F,Cortado H,Rangel-Moreno J,Justice SS,Yang C,Spencer JD,Becknell B,Partida-Sanchez S

17-5920-82 was used in Flow Cytometry to reveal a previously unappreciated outcome for neutrophil-macrophage imbalance in promoting host susceptibility to acute pyelonephritis and the development of permanent renal damage.

Fri Jan 01 00:00:00 EST 2021

AXL Is a Key Factor for Cell Plasticity and Promotes Metastasis in Pancreatic Cancer.

Molecular cancer research : MCR

Du W,Phinney NZ,Huang H,Wang Z,Westcott J,Toombs JE,Zhang Y,Beg MS,Wilkie TM,Lorens JB,Brekken RA

17-5920-82 was used in Western Blot to implicate AXL as a marker of undifferentiated PDA cells and a target for therapy.

Sun Aug 01 00:00:00 EDT 2021

Prostaglandin E₃ attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

Journal of cellular and molecular medicine

Cui J,Shan K,Yang Q,Qi Y,Qu H,Li J,Wang R,Jia L,Chen W,Feng N,Chen YQ

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction.

Experimental and therapeutic medicine

Zhang M,Yang JK,Ma J

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization.

Stem cell research & therapy

Dong B,Wang C,Zhang J,Zhang J,Gu Y,Guo X,Zuo X,Pan H,Hsu AC,Wang G,Wang F

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Wed Mar 24 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation.

Nature communications

Iizasa E,Chuma Y,Uematsu T,Kubota M,Kawaguchi H,Umemura M,Toyonaga K,Kiyohara H,Yano I,Colonna M,Sugita M,Matsuzaki G,Yamasaki S,Yoshida H,Hara H

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Computational modeling reveals a key role for polarized myeloid cells in controlling osteoclast activity during bone injury repair.

Scientific reports

Lo CH,Baratchart E,Basanta D,Lynch CC

17-5920 was used in Flow cytometry/Cell sorting to underscore the value of mathematical modeling approaches to understand and reveal new insights into complex biological processes.

Mon Mar 15 00:00:00 EDT 2021

Computational modeling reveals a key role for polarized myeloid cells in controlling osteoclast activity during bone injury repair.

Scientific reports

Lo CH,Baratchart E,Basanta D,Lynch CC

17-5920 was used in Flow cytometry/Cell sorting to underscore the value of mathematical modeling approaches to understand and reveal new insights into complex biological processes.

Mon Mar 15 00:00:00 EDT 2021

3D Model of the Early Melanoma Microenvironment Captures Macrophage Transition into a Tumor-Promoting Phenotype.

Cancers

Pizzurro GA,Liu C,Bridges K,Alexander AF,Huang A,Baskaran JP,Ramseier J,Bosenberg MW,Mak M,Miller-Jensen K

17-5920-82 was used in Flow cytometry/Cell sorting to develop 3D in vitro co-culture systems, composed of a collagen-I matrix scaffolding murine bone-marrow-derived macrophages (BMDMs), YUMM1.7 melanoma cells, and fibroblasts to recreate the early melanoma TME and study how interactions with fibroblasts and tumor cells modulate macrophage immune activity.

Sun Sep 12 00:00:00 EDT 2021

Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.

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Weinstock NI,Shin D,Dhimal N,Hong X,Irons EE,Silvestri NJ,Reed CB,Nguyen D,Sampson O,Cheng YC,Lau JTY,Bongarzone ER,Kofler J,Escolar ML,Gelb MH,Wrabetz L,Feltri ML

17-5920 was used in Flow cytometry/Cell sorting to show that cross-correction does not occur efficiently in vivo and that Galc-deficient Schwann cells autonomously produce psychosine.

Wed Jul 08 00:00:00 EDT 2020

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling.

PLoS pathogens

Still KM,Batista SJ,O'Brien CA,Oyesola OO,Früh SP,Webb LM,Smirnov I,Kovacs MA,Cowan MN,Hayes NW,Thompson JA,Tait Wojno ED,Harris TH

17-5920 was used in Flow cytometry/Cell sorting to show that IL-33 is expressed by oligodendrocytes and astrocytes during T. gondii infection, is released locally into the cerebrospinal fluid of T. gondii-infected animals, and is required for control of infection.

Thu Oct 01 00:00:00 EDT 2020

Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation.

Oxidative medicine and cellular longevity

Lee CB,Seo IH,Chae MW,Park JW,Choi EH,Uhm HS,Baik KY

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy.

Nature communications

Baumann D,Hägele T,Mochayedi J,Drebant J,Vent C,Blobner S,Noll JH,Nickel I,Schumacher C,Boos SL,Daniel AS,Wendler S,Volkmar M,Strobel O,Offringa R

17-5920 was used in Flow cytometry/Cell sorting to show that targeted inhibition of mitogen-activated protein kinase (MAPK) kinase (MEK) does not impair dendritic cell-mediated T cell priming and activation.

Fri May 01 00:00:00 EDT 2020

Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy.

Nature communications

Baumann D,Hägele T,Mochayedi J,Drebant J,Vent C,Blobner S,Noll JH,Nickel I,Schumacher C,Boos SL,Daniel AS,Wendler S,Volkmar M,Strobel O,Offringa R

Fri May 01 00:00:00 EDT 2020

CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury.

Cell reports

Giri J,Das R,Nylen E,Chinnadurai R,Galipeau J

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Select autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes.

Nature microbiology

Wang YT,Zaitsev K,Lu Q,Li S,Schaiff WT,Kim KW,Droit L,Wilen CB,Desai C,Balce DR,Orchard RC,Orvedahl A,Park S,Kreamalmeyer D,Handley SA,Pfeifer JD,Baldridge MT,Artyomov MN,Stallings CL,Virgin HW

17-5920 was used in Flow cytometry/Cell sorting to delineate a function of the autophagy proteins Beclin 1 and FIP200-but not of other essential autophagy components ATG5, ATG16L1 or ATG7-in mediating quiescence of tissue-resident macrophages by limiting the effects of systemic interferon-γ.

Sat Feb 01 00:00:00 EST 2020

Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.

Nature communications

Kurelac I,Iommarini L,Vatrinet R,Amato LB,De Luise M,Leone G,Girolimetti G,Umesh Ganesh N,Bridgeman VL,Ombrato L,Columbaro M,Ragazzi M,Gibellini L,Sollazzo M,Feichtinger RG,Vidali S,Baldassarre M,Foriel S,Vidone M,Cossarizza A,Grifoni D,Kofler B,Malanchi I,Porcelli AM,Gasparre G

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Fri Feb 22 00:00:00 EST 2019

Irg1 expression in myeloid cells prevents immunopathology during M. tuberculosis infection.

The Journal of experimental medicine

Nair S,Huynh JP,Lampropoulou V,Loginicheva E,Esaulova E,Gounder AP,Boon ACM,Schwarzkopf EA,Bradstreet TR,Edelson BT,Artyomov MN,Stallings CL,Diamond MS

Mon Apr 02 00:00:00 EDT 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression.

Oncotarget

Simonneau M,Frouin E,Huguier V,Jermidi C,Jégou JF,Godet J,Barra A,Paris I,Levillain P,Cordier-Dirikoc S,Pedretti N,Bernard FX,Lecron JC,Morel F,Favot L

17-5920 was used in Flow cytometry/Cell sorting to examine the contribution of oncostatin M to the progression of cutaneous squamous cell carcinoma.

Fri Nov 23 00:00:00 EST 2018

Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression.

Oncotarget

Simonneau M,Frouin E,Huguier V,Jermidi C,Jégou JF,Godet J,Barra A,Paris I,Levillain P,Cordier-Dirikoc S,Pedretti N,Bernard FX,Lecron JC,Morel F,Favot L

17-5920 was used in Flow cytometry/Cell sorting to examine the contribution of oncostatin M to the progression of cutaneous squamous cell carcinoma.

Fri Nov 23 00:00:00 EST 2018

Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency.

Nature cell biology

Giampazolias E,Zunino B,Dhayade S,Bock F,Cloix C,Cao K,Roca A,Lopez J,Ichim G,Proïcs E,Rubio-Patiño C,Fort L,Yatim N,Woodham E,Orozco S,Taraborrelli L,Peltzer N,Lecis D,Machesky L,Walczak H,Albert ML,Milling S,Oberst A,Ricci JE,Ryan KM,Blyth K,Tait SWG

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

The pattern recognition receptor, Mincle, is essential for maintaining the M1 macrophage phenotype in acute renal inflammation.

Kidney international

Lv LL,Tang PM,Li CJ,You YK,Li J,Huang XR,Ni J,Feng M,Liu BC,Lan HY

17-5920-82 was used in Flow cytometry/Cell sorting to investigate the role of Mincle (macrophage-inducible C-type lectin, Clec4e) in kidney disease.

Wed Mar 01 00:00:00 EST 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TGF-β receptor 1 inhibition prevents stenosis of tissue-engineered vascular grafts by reducing host mononuclear phagocyte activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lee YU,de Dios Ruiz-Rosado J,Mahler N,Best CA,Tara S,Yi T,Shoji T,Sugiura T,Lee AY,Robledo-Avila F,Hibino N,Pober JS,Shinoka T,Partida-Sanchez S,Breuer CK

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection.

Cell reports

Schleicher U,Paduch K,Debus A,Obermeyer S,König T,Kling JC,Ribechini E,Dudziak D,Mougiakakos D,Murray PJ,Ostuni R,Körner H,Bogdan C

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2016

Dendritic cell-derived nitric oxide inhibits the differentiation of effector dendritic cells.

Oncotarget

Si C,Zhang R,Wu T,Lu G,Hu Y,Zhang H,Xu F,Wei P,Chen K,Tang H,Yeretssian G,Xiong H

17-5920 was used in Flow cytometry/Cell sorting to demonstrate that DC-derived nitric oxide restrains effector DC development to treat autoimmune and inflammatory diseases.

Tue Nov 15 00:00:00 EST 2016

Commensal Gram-positive bacteria initiates colitis by inducing monocyte/macrophage mobilization.

Mucosal immunology

Nakanishi Y,Sato T,Ohteki T

Thu Jan 01 00:00:00 EST 2015

Expression of Cationic Amino Acid Transporter 2 Is Required for Myeloid-Derived Suppressor Cell-Mediated Control of T Cell Immunity.

Journal of immunology (Baltimore, Md. : 1950)

Cimen Bozkus C,Elzey BD,Crist SA,Ellies LG,Ratliff TL

17-5920 was used in Flow cytometry/Cell sorting to investigate whether restricting myeloid-derived suppressor cell uptake of l-Arginine is a critical control point to modulate their suppressor activity.

Tue Dec 01 00:00:00 EST 2015

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

Published figure using iNOS monoclonal antibody (Product # 17-5920-82) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Myeloid cell-derived inducible nitric oxide synthase suppresses M1 macrophage polarization.

Nature communications

Lu G,Zhang R,Geng S,Peng L,Jayaraman P,Chen C,Xu F,Yang J,Li Q,Zheng H,Shen K,Wang J,Liu X,Wang W,Zheng Z,Qi CF,Si C,He JC,Liu K,Lira SA,Sikora AG,Li L,Xiong H

17-5920 was used in Flow cytometry/Cell sorting to show that iNOS-deficient mice display enhanced classically activated M1 macrophage polarization without major effects on alternatively activated M2 macrophages.

Fri Mar 27 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

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Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a

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UniProt ID: (Mouse) P29477

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Entrez Gene ID: (Mouse) 18126

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Function(s)

actin binding NADPH-hemoprotein reductase activity nitric-oxide synthase activity receptor binding iron ion binding protein binding calmodulin binding beta-catenin binding cAMP-dependent protein kinase regulator activity FMN binding oxidoreductase activity protein kinase binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity cadherin binding metal ion binding flavin adenine dinucleotide binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase

Process(es)

ovulation from ovarian follicle response to hypoxia arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process inflammatory response nitric oxide mediated signal transduction negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium negative regulation of blood pressure regulation of protein kinase activity positive regulation of vasodilation regulation of insulin secretion positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma

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iNOS Monoclonal Antibody (CXNFT), APC, eBioscience™

iNOS Antibody (17-5920-82) in Flow

Product Details

17-5920-82

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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