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Invitrogen
iNOS Monoclonal Antibody (CXNFT), Brilliant Ultra Violet™ 563, eBioscience™
FIGURE: 1 / 1
iNOS Antibody (365-5920-82) in Flow
BALB/c mouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS (Product # 00-4976-03). Cells were surface-stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) and then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.125 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet 563 (Product # 365-4321-81) (left) or 0.125 µg of iNOS Monoclonal Antibody, Brilliant Ultra Violet 563 (right). Total viable cells ... View More
Product Details
365-5920-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CXNFT
Conjugate
Excitation/Emission Max
349/563 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2942126
Product Specific Information
Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.
Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This CXNFT antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye that emits at 564 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a
UniProt ID: (Mouse) P29477
Entrez Gene ID: (Mouse) 18126
actin binding
NADPH-hemoprotein reductase activity
nitric-oxide synthase activity
receptor binding
iron ion binding
protein binding
calmodulin binding
beta-catenin binding
cAMP-dependent protein kinase regulator activity
FMN binding
oxidoreductase activity
protein kinase binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
cadherin binding
metal ion binding
flavin adenine dinucleotide binding
nitric-oxide synthase binding
Hsp90 protein binding
oxidoreductase
ovulation from ovarian follicle
response to hypoxia
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
inflammatory response
nitric oxide mediated signal transduction
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to lipopolysaccharide
cellular response to drug
regulation of cell proliferation
negative regulation of protein catabolic process
defense response to bacterium
negative regulation of blood pressure
regulation of protein kinase activity
positive regulation of vasodilation
regulation of insulin secretion
positive regulation of killing of cells of other organism
oxidation-reduction process
cellular response to lipopolysaccharide
cellular response to interferon-gamma
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