Invitrogen

iNOS Monoclonal Antibody (CXNFT), APC-eFluor™ 780, eBioscience™

18 Published Figures

19 References

View all (65) iNOS antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (18)

FIGURE: 1 / 19

iNOS Antibody (47-5920-82) in Flow

Mouse thioglycolate-elicited peritoneal exudate cells were unstimulated (left) or stimulated overnight with LPS (Product #00-4976) (right) then surface stained with Anti-Mouse F4/80 FITC (Product # 11-4801). Cells were fixed and permeabilized using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824) then intracellularly stained with 0.03 µg of Anti-Mouse NOS2 APC-eFluor® 780 (Product # 47-5920-82). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863), were used for analysis.

Product Image Gallery

Product Details

47-5920-82

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CXNFT

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2716962

Product Specific Information

Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.

Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This CXNFT antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal exudate cells using the intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. The Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) may also be used with similar results. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

High-Dose Acetaminophen with N-acetylcysteine Rescue Inhibits M2 Polarization of Tumor-Associated Macrophages.

Cancers

Bryan A,Pingali P,Joslyn M,Li H,Bernas T,Koblinski J,Landry J,Lee WS,Patel B,Neuwelt A

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Acute murine cytomegalovirus infection boosts cell-type specific response and lipid metabolism changes in the liver of infant mice.

Frontiers in immunology

Gao J,Wang A,Bu X,Jia W

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Tue Aug 29 00:00:00 EDT 2023

Th2 cells inhibit growth of colon and pancreas cancers by promoting anti-tumorigenic responses from macrophages and eosinophils.

British journal of cancer

Jacenik D,Karagiannidis I,Beswick EJ

47-5920-82 was used in Immunohistochemistry (PFA fixed) to demonstrate the significance of Th2 cells, eosinophils and IL-5 in the inhibition of gastrointestinal tumor growth, and pointed toward tumor microenvironment reprogramming as a Th2 cell-mediated anti-tumorigenic mechanism of action.

Sun Jan 01 00:00:00 EST 2023

MK2 Promotes the Development and Progression of Pancreatic Neuroendocrine Tumors Mediated by Macrophages and Metabolomic Factors.

International journal of molecular sciences

Jacenik D,Lebish EJ,Beswick EJ

47-5920-82 was used in flow cytometry to identify MK2 as a potent driver of immune response and metabolic effectors in PNETs, suggesting it is a potential therapeutic target for patients with PNETs.

Sat Nov 05 00:00:00 EDT 2022

Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells.

Immunity

Formaglio P,Alabdullah M,Siokis A,Handschuh J,Sauerland I,Fu Y,Krone A,Gintschel P,Stettin J,Heyde S,Mohr J,Philipsen L,Schröder A,Robert PA,Zhao G,Khailaie S,Dudeck A,Bertrand J,Späth GF,Kahlfuß S,Bousso P,Schraven B,Huehn J,Binder S,Meyer-Hermann M,Müller AJ

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Tue Dec 14 00:00:00 EST 2021

Prostaglandin E₃ attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

Journal of cellular and molecular medicine

Cui J,Shan K,Yang Q,Qi Y,Qu H,Li J,Wang R,Jia L,Chen W,Feng N,Chen YQ

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction.

Experimental and therapeutic medicine

Zhang M,Yang JK,Ma J

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization.

Stem cell research & therapy

Dong B,Wang C,Zhang J,Zhang J,Gu Y,Guo X,Zuo X,Pan H,Hsu AC,Wang G,Wang F

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Wed Mar 24 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation.

Nature communications

Iizasa E,Chuma Y,Uematsu T,Kubota M,Kawaguchi H,Umemura M,Toyonaga K,Kiyohara H,Yano I,Colonna M,Sugita M,Matsuzaki G,Yamasaki S,Yoshida H,Hara H

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Computational modeling reveals a key role for polarized myeloid cells in controlling osteoclast activity during bone injury repair.

Scientific reports

Lo CH,Baratchart E,Basanta D,Lynch CC

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.

British journal of pharmacology

De Cicco P,Ercolano G,Rubino V,Terrazzano G,Ruggiero G,Cirino G,Ianaro A

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy.

Nature communications

Baumann D,Hägele T,Mochayedi J,Drebant J,Vent C,Blobner S,Noll JH,Nickel I,Schumacher C,Boos SL,Daniel AS,Wendler S,Volkmar M,Strobel O,Offringa R

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury.

Cell reports

Giri J,Das R,Nylen E,Chinnadurai R,Galipeau J

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression.

Oncotarget

Simonneau M,Frouin E,Huguier V,Jermidi C,Jégou JF,Godet J,Barra A,Paris I,Levillain P,Cordier-Dirikoc S,Pedretti N,Bernard FX,Lecron JC,Morel F,Favot L

Published figure using iNOS monoclonal antibody (Product # 47-5920-82) in Flow Cytometry

Fri Nov 23 00:00:00 EST 2018

Bioinformatics

Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

View more View less

Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a

View more View less

UniProt ID: (Mouse) P29477

View more View less

Entrez Gene ID: (Mouse) 18126

View more View less

Function(s)

actin binding NADPH-hemoprotein reductase activity nitric-oxide synthase activity receptor binding iron ion binding protein binding calmodulin binding beta-catenin binding cAMP-dependent protein kinase regulator activity FMN binding oxidoreductase activity protein kinase binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity cadherin binding metal ion binding flavin adenine dinucleotide binding nitric-oxide synthase binding Hsp90 protein binding oxidoreductase

Process(es)

ovulation from ovarian follicle response to hypoxia arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process inflammatory response nitric oxide mediated signal transduction negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium negative regulation of blood pressure regulation of protein kinase activity positive regulation of vasodilation regulation of insulin secretion positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

iNOS Monoclonal Antibody (CXNFT), APC-eFluor™ 780, eBioscience™

iNOS Antibody (47-5920-82) in Flow

Product Details

47-5920-82

Immunohistochemistry (PFA fixed) (IHC (PFA))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!