Brilliant Ultra Violet 395 excitation shown as dotted line and emission shown as solid violet histogram
3355379/28347399(in buffer) 5
flow cytometry

Invitrogen Brilliant Ultra Violet™ 395 (BUV395) dye is excited by the 350 nm UV laser and emits at 395 nm. This dye has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BUV395 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Ultra Violet™ 395 primary antibodies

Experimental data using Brilliant Ultra Violet™ 395

Spectral signature of Brilliant Ultra Violet™ 395 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 395 were used for analysis.

 

Dot plot of IFN-gamma BUV395 vs CD4 FITC in unstimulated and stimulated cells

Intracellular staining of human peripheral blood cells using Brilliant Ultra Violet™ 395. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (right). Cells were then stained intracellularly, using Intracellular Fixation & Permeabilization Buffer Set, CD4 Monoclonal Antibody (RPA-T4), FITC and IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 395. Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

Dot plot of samples stained with IgG1 control vs CD19-FITC and CD3-BUV395 vs CD19-FITC

Cell surface staining of human peripheral blood cells using Brilliant Ultra Violet™ 395. Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 395 (left) or CD3 Monoclonal Antibody, Brilliant Ultra Violet™ 395 (right). Cells in the lymphocyte gate were used for analysis.

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.

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