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Roswell Park Memorial Institute (RPMI) 1640 Medium
Culture your cells with Gibco RPMI 1640 medium
Roswell Park Memorial Institute (RPMI) 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins, including certain vitamins not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium.
Gibco RPMI 1640 Medium has been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI composition
RPMI 1640 Medium contains the reducing agent glutathione as well as biotin, vitamin B12, and PABA, which are vitamins not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, RPMI 1640 media includes high concentrations of the vitamins inositol and choline. Because RPMI 1640 contains no proteins, lipids, or growth factors, it is commonly supplemented with fetal bovine serum (FBS). The sodium bicarbonate buffer system used in RPMI 1640 allows for maintenance of physiological pH in a 5–10% CO2 environment.
Thermo Fisher Scientific offers a variety of modifications to RPMI composition for a range of cell culture applications—view an example of a detailed RPMI formulation here. Learn about additional RPMI formulation options using our Cell Culture Media Formulation Tool.
Choose the right RPMI 1640
There are several factors to consider when selecting the right Gibco RPMI composition for your cell culture experiments, including format, supplementation, and additives. Our interactive media formulation tool provides side-by-side comparisons of RPMI 1640 formulations, allowing for easy selection of the RPMI 1640 you need. Select the following modifications:
- L-glutamine / GlutaMAX supplementation
- Phenol red indicator
- Liquid vs powder formats
- Serum supplementation
- Sodium pyruvate additive
- Glucose additive
- HEPES additive
Innovative RPMI 1640 formulations
Take your cell culture to the next level with enhanced RPMI 1640 formulations. Explore the following formulations that can help improve your cell viability, serum supplementation, and cold storage constraints.
Advanced RPMI 1640 Medium
Gibco Advanced RPMI 1640 Medium is formulated with ingredients that reduce the need for serum supplementation by 50–90%, lowering your costs and increasing consistency in your cell culture with fewer lot-to-lot serum changes.
BenchStable RPMI 1640
Gibco BenchStable RPMI 1640 Medium is engineered for storage at room temperature, saving your cold storage space while supporting cell proliferation and densities comparable to standard RPMI 1640 formulations.
RPMI 1640 Medium with GlutaMAX supplement
Gibco RPMI 1640 with GlutaMAX supplement contains a stable form of L-glutamine that does not spontaneously degrade in culture, preventing ammonia build up and improving cell health and viability.
Customize your RPMI 1640
If you require a custom RPMI 1640 formulation for your cell culture experiments, our media customization services can provide you with the media you need, including custom components, packaging, and QC testing.
GMP manufacturing
Gibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, helping to ensure the consistency, reliability, and high quality you can rely on.
Sustainable solutions
We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.
Resources
Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.
Gibco Cell Culture Basics
Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.
Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.
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Support
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support? Contact our expert team for technical and application support of Laboratory Products.
For Research Use Only. Not for use in diagnostic procedures.