Lipofectamine 3000 Transfection Reagent

When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1).

All four steps in the transfection process were optimized and our most advanced lipid nanoparticle technologies were combined. This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line.

Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. They are easy to culture and are commonly used in transfection and large-scale production of therapeutic proteins. Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments.

Using the most biologically relevant cell lines helps provide the most relevant answers to your research questions. The exceptional cell spectrum and high transfection efficiency of Lipofectamine 3000 reagent enables excellent flexibility to use your cell line of choice in your studies.

Compare Lipofectamine 3000 reagent and Lipofectamine 2000 reagent in a variety of cell lines using the comparison charts below. Find Lipofectamine 3000 reagent transfection efficiency in each cell line as well as fold protein expression improvement when using Lipofectamine 3000 over Lipofectamine 2000 reagent.

For exceptional performance, utilize Lipofectamine 3000 to enhance transfection in your cancer research experiments. This advanced and versatile reagent works well for transfection in breast, lung, prostate, and liver cancer cells. There are step-by-step protocols to help ensure high transfection efficiency with low toxicity when a specific cancer cell line is coupled with Lipofectamine 3000 transfection reagent.

Lipofectamine 3000 reagent demonstrates significantly higher transfection performance compared to leading transfection reagents, such as Lipofectamine 2000 reagent, in a panel of cancer cells derived from various tissues. While only 25% of the selected cancer cell line panel would be considered easy to transfect (>50% transfection efficiency) with Lipofectamine 2000 reagent, 60% of the panel is easy to transfect with Lipofectamine 3000 reagent.

The exceptional transfection efficiency of Lipofectamine 3000 reagent in conjunction with Invitrogen Epi5 Episomal iPSC Reprogramming Kit enables highly efficient reprogramming of somatic cells without the need for electroporation.

Lipofectamine 3000 reagent helps break through the boundaries of delivery and facilitates new technologies, such as genome engineering, in more biologically relevant systems. With this reagent, TALENs and CRISPR sequences target the AAVS1 locus in HepG2 and U2OS cells, and show improved transfection efficiency, mean fluorescence intensity, and genomic cleavage. These advancements in delivery help minimize painstaking downstream workflows, enable easier stem cell manipulation, and enhance site-specific insertion of transgenes into the cellular genome.

 Download Lipofectamine 3000 application note