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Applied Biosystems TaqMan master mixes have been optimized for use across a wide variety of research and discovery-stage biopharmaceutical applications. Choosing the right master mix depends on your target (RNA or DNA), application, test format, and multiplexing needs. For most qPCR research applications, we recommend using either TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mixes
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Key applications |
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Target |
DNA, including cDNA |
RNA |
Concentration |
2X |
4X |
Multiplexing and passive reference dye |
With ROX: up to 2 targets |
Available in 2 versions: With ROX: up to 3 targets No ROX: 3 or more targets |
Cycling mode/run time |
Fast: ~40 min Standard: ~50 min |
Fast: ~60 min Standard: ~90 min |
Carryover contamination control |
Available in 2 versions: |
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Reverse transcriptase |
Purchase SuperScript IV VILO Master Mix separately and use before qPCR |
Thermostable MMLV RT included in master mix |
Assay recommendations |
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For Research Use Only. Not for use in diagnostic procedures.