Very long DNA fragments typically cannot be amplified using standard PCR enzymes. We offer a range of solutions enabling amplification of very long fragments with high-fidelity and/or speed.

Thermo Scientific long-range PCR enzyme selection table

 Phusion Hot Start II High-Fidelity DNA PolymerasePhire Hot Start II DNA Polymerase
Amplicon sizeUp to 20 kbUp to 20 kb
Fidelity vs. Taq52x2x
Enhanced specificity✓✓
Extension time15-30 s/kb15 s/kb
Stand-alone enzyme
Master mix

*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

Additional long-range PCR enzymes

Resources

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For Research Use Only. Not for use in diagnostic procedures.