Gibco EfficientFeed+ Supplements

No more compromise

Until now, traditional fed-batch bioreactor space necessitated a compromise: the amount of complete feeding supplements one could add meant a trade-off between initial working volume and feeding space (dilution factor).

Today, EfficientFeed + Supplements empower you to deliver up to 200 g/L of needed nutrients in a complete, super-concentrated, pH-neutral feed. The reduction in required feeding space provides you with three ways to optimize your bioreactor output for every run:

1. Reduce product dilution
2. Maximize working volume
3. Increase feeding of nutrients for increased titer

Feeding examples (traditional bolus feeding):

Increase your bioprocess productivity

Gibco EfficientFeed + Supplements are designed to deliver the same 2- to 5-fold increases in titer possible with original EfficientFeed supplements, plus the flexibility to be super-concentrated up to 3X (or 200 g/L) of original levels while remaining pH-neutral and chemically defined.

In Figure 1, we demonstrate an improvement in titer with super-concentrated EfficientFeed C+ supplement.

We made EfficientFeed Supplements even better

Gibco EfficientFeed+ Supplements represent an improved version of earlier formulations. The result is improved titers relative to supplements from other vendors.

Figure 2 illustrates how titer comparisons for cell cultures and demonstrate how well EfficientFeed+ Supplements perform. A customer completed a head-to-head comparison in fed-batch culture of a DG44 cell line expressing IgG and demonstrated superior titers over approximately 20 days (peak titers at 80% harvest viability). In that comparison, EfficientFeed + Supplements demonstrated performance up to 139% better than those from other suppliers.

Figure 2. Titer comparison with CD FortiCHO medium and EfficientFeed C+ and other commercially available feeds on the market. EfficientFeed+ Supplements work better than the original versions. 

Reliable product quality

Figure 3 shows how product quality profiles remain consistent whether increasing feeding percentage or when switching from the original Efficientfeed formulation to the EfficientFeed+ Supplements. These improved formulations allow you to increase your bioreactor’s working volume. Feed more and achieve higher titers without sacrificing product quality.

Figure 3. Relative concentration of product quality profiles stays consistent when increasing feeding percentage or when switching from original EfficientFeed to EfficientFeed+ products.  

Figure 4. EfficientFeed+ concentrate supplement with CHO-S average growth

Figure 5.  EfficientFeed+ concentrate supplement with CHO-S average titer

Simple preparation helps reduce risk

Our AGT dry media format provides ease-of-use while helping you reduce risk by minimizing the numerous steps and adjustments previously required to prepare conventional media.

Storage of reconstituted liquid also reduces risk

Reconstituted EfficientFeed+ Supplements can be stored at room temperature (in the dark) for the period of a typical fed-batch bioreactor run. Making just one closed-system connection per bioreactor run reduces the opportunities for an error that could lead to contamination.