Seven shake flasks on a white surface, six are filled with clear medium while one has phenol-red treated liquid. All have blue caps.

Shake flask features

Thermo Scientific single-use cell culture Erlenmeyer flasks are excellent for suspension culture and media preparation. Ready for mixing and storage and featuring crystal-clear polyethylene terephthalate glycol (PETG), these single-use flasks are robust and break resistant.

Standard non-vented closures help prevent spilling and contamination, and optional vented closures (0.22 µm) improves gas exchange, especially beneficial for suspension cell culture.

NEW Flasks for maximizing suspension culture and protein expression scale-up

See how the 5-Liter flask can help save you time, money & space while helping reduce the media and plastics used in a lab all while helping increase efficiency for suspension culturing.

Our angled flask (Cat. No. 4115-5000) has also shown to be the only flask tested capable generating larger protein titers in CHO cells due to its proprietary design (see Figure 1).

5L shake flask containing ExpiCHO cells on a Thermo Scientific orbital shaker
Bar chart of protein production in 4 flasks
Figure 1. Protein expression levels in 5 L shake flasks from different suppliers. Protein titers were determined for ExpiCHO cells transfected in a 1.5 L starting volume using the Max Titer (32°C) protocol with a three-feed strategy in 5 L shake flasks (N = 2).

Don’t take our word for it. See our application notes regarding how to help increase your potential for each Expi kit.

Product features include:

  • Molded-in graduation for accurate volume assessment
  • Non-pyrogenic, non-cytotoxic
  • Optional vented caps for gas exchange
  • Gamma irradiation sterilization (per ISO 1137-2)
  • DNase-, RNase-, and human DNA-free
  • BSE and TSE risk-free

Shake flask volume information

CapacityOuter diameterCase size
125 mL66.3 mm24
250 mL82.6 mm12
500 mL101.6 mm12
1,000 mL129.8 mm6
2,000 mL161.5 mm4
2,800 mL203.2 mm4
5,000 mL214 mm4


Order sterile single-use shake flasks

product photo

Separated below by cap type, sterile single-use shake flasks are available with plain and baffled bottoms. These sterile single-use Erlenmeyer flasks for cell culture are:

  • Baffled-bottom flasks are designed to provide additional aeration and gas exchange with low shear to help maintain cell viability
  • Sterile and single use to help reduce contamination risk
  • BPA-free
  • Collapsible when autoclaved, helps reduce biohazardous waste
  • Graduated to enable rapid volume assessment
Sterile shake flasks with vented caps
Sterile shake flasks with solid, non-vented caps

Resources

Stylesheet for Classic Wide Template adjustments