3D cancer research starts with quality cancer organoid cell lines

Kick-off your 3D cancer research workflow with our Gibco OncoPro Tumoroid Cell Lines. Also referred to as cancer organoid cell lines, these models are established from primary patient tissues and have been fully characterized. Each of our tumoroid cell lines were derived and expanded in our Gibco OncoPro Tumoroid Culture Medium, and are accompanied by detailed protocols and resources to help with adoption and integration into your research workflow.

When cultured in OncoPro Tumoroid Culture Medium, OncoPro Tumoroid Cell Lines help support quality 3D cancer research since these lines are:

  • Biologically relevant—preferential expansion of patient-derived cancer cells from diverse cancer indications
  • Stable—demonstrated to maintain donor-specific phenotype and genotype in long-term culture
  • Scalable—compatible with a readily-scalable suspension culture method


Available OncoPro Tumoroid Cell Lines

Tumoroid lineDiagnosis - StageTumor siteRace Sex, AgeHeight (in), Weight (lb)Treatment status
HuCo031721Colorectal adenocarcinoma - IIATransverse colonWhite Male, 8270", 260 lbPre-Treatment
HuCo111622Colorectal adenocarcinoma - IIARectumWhite Female, 7063", 215 lbPre-Treatment
HuCo083123Colorectal adenocarcinoma - IIICRectosigmoidWhite Male, 4670", 248 lbPre-Treatment
HuCo090123Poorly differentiated adenocarcinoma - IIAColonAfrican-American Female, 9063", 138 lbPre-Treatment
HuCo100523Colorectal adenocarcinoma - IIAAscending colonWhite Female, 8562", 148 lbPre-Treatment
HuCo052121Colorectal adenocarcinoma - IIARectumWhite Female, 4660", 154 lbPre-Treatment
HuLu051421NSCLC, Squamous cell carcinoma - IA3Middle lobe of lungWhite Male, 6375", 209 lbPre-Treatment
HuLu051921NSCLC, Large cell carcinoma - IIAUpper lobe of lungWhite Female, 6967", 169 lbPre-Treatment
HuBr102622Invasive ductal carcinoma, ductal carcinoma in situ present - IABreastWhite Female, 5663", 131 lbPre-Treatment
HuEn061022Endometrioid Carcinoma - IAEndometriumWhite Female, 6765", 162 lbPre-Treatment
HuEn070722Dedifferentiated carcinoma - IIIC1Endometrium, lower uterine segmentWhite Female, 7666", 272 lbPre-Treatment
HuEn041521Endometrioid Carcinoma - IIIC1EndometriumWhite Female, 7860", 189 lbPre-Treatment
HuEn022222Endometrioid carcinoma - IAEndometriumWhite Female, 5862", 150 lbPre-Treatment
HuEn033122Endometrioid carcinoma - IAEndometriumWhite Female, 5964", 200 lbPre-Treatment
Contact our team to request more information or learn how to purchase available tumoroid cell lines by submitting an inquiry.

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Tumoroid cell line data

Explore relevant data that demonstrate the quality and consistency of OncoPro Tumoroid Cell Lines. In-depth characterization data is available for each tumoroid cell line upon request by completing an inquiry form.

Biological relevance of tumoroid cell lines


Stability of tumoroid lines

Figure 2. OncoPro Tumoroid Cell Lines maintain major characteristics during long-term culture in OncoPro Tumoroid Culture Medium. (A) Cumulative population doubling (PD) was measured by cell counts at each passage. Tumoroid lines were recovered from cryopreservation at the beginning of this experiment (day 0), and additional cryopreservation and recovery points are indicated by arrows for each culture. (B) Representative images of tumoroid morphology before and after prolonged culture. (C) Correlation of germline and somatic single nucleotide variations (SNVs) between early and later passage tumoroid cultures. Each dot represents the variant allelic frequency (VAF) for one genetic locus covered by the Oncomine Comprehensive Assay v3. (D) Differential gene expression analysis of early versus late passage tumoroids reveals a modest number of differentially expressed genes (DEGs) at fold change >2 and false discovery rate (FDR) <0.05. Gene ontology analysis revealed a few significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways (FDR <0.05) between early and late passage tumoroid cultures; the top 10 results are shown.


Scalability of tumoroid lines

Figure 3. Tumoroid lines grow consistently in an easy-to-use, scalable format.(A) Expansion rates of tumoroids in suspension culture in OncoPro medium. Number of cells counted after thawing from cryopreservation (day 0) and at each passage. (B) Individual samples have consistent growth rates and can typically generate 100 million viable cells within 30 or 60 days.


Additional tumoroid resources

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For Research Use Only. Not for use in diagnostic procedures.

Stylesheet for Classic Wide Template adjustments
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