Dulbecco's Modified Eagle Medium F12 (DMEM F12)

Culture your cells with Gibco DMEM F12

 

Gibco Dulbecco's Modified Eagle Medium: Nutrient Mixture F12 (DMEM F12) is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. DMEM F12 is a 1:1 mixture of DMEM and Ham's F12 media. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with the wide variety of components found in Ham's F12 media.

 

Gibco DMEM F12 is formulated for superior quality and delivers greater reliability, consistency, and improved control in mammalian cell culture.

DMEM F-12 composition

Along with the glucose, amino acids, and vitamins in DMEM, DMEM F12 contains additional components found in Ham’s F12 media, including zinc, putrescine, hypoxanthine, and thymidine. DMEM F12 may also include other factors such as L-glutamine and phenol red indicator. However, due to its lack of proteins, lipids, and growth factors, DMEM F12 is commonly supplemented with fetal bovine serum (FBS). In addition, the sodium bicarbonate buffer system used in DMEM F12 requires a 5–10% CO2 environment for maintenance of physiological pH.

 

Thermo Fisher Scientific offers a variety of DMEM F12 modifications for a range of cell culture applications—view an example of a detailed DMEM/F12 formulation here. Learn about additional DMEM/F12 formulation options in the Gibco Media selection tool.

DMEM F-12 vs DMEM

DMEM alone is rich in nutrients such as amino acids and vitamins, while Ham’s F12 media contains a higher diversity of components. Therefore, DMEM F12 is a more complex media than DMEM, including many additional factors (e.g., zinc, thymidine) that are generally not found in more standard basal media formulations. 

Choose the right DMEM F12

There are several factors to consider when selecting the right Gibco DMEM F12 composition
for your cell cultures, including format, supplementation, and additives. Our interactive
media selection tool helps enable easy selection of the DMEM F12 you need.

Innovative DMEM F12 formulations

Take your cell culture to the next level with enhanced DMEM formulations. Explore the following formulations that can help improve your cell viability, serum supplementation, cold storage constraints, and clinical cell culture.

Advanced DMEM F12

Gibco Advanced DMEM F12 includes ingredients that allow you to reduce FBS supplementation by 50–90%. This both helps reduce your costs and increase consistency in your experiments with fewer lot-to-lot serum changes.
 

BenchStable DMEM F12

Gibco BenchStable DMEM F12 is formulated for storage at room temperature, helping save valuable cold storage space while maintaining the ability to support cell proliferation and densities at levels comparable to standard DMEM F12.
 

DMEM F12 with GlutaMAX supplement

Gibco DMEM F12 with GlutaMAX supplement allows you to provide your cells with a stable source of L-glutamine that does not spontaneously degrade, helping improve cell health and viability by reducing toxic ammonia buildup.
 

CTS Knockout DMEM F12

Taking your work to the clinic? Gibco Cell Therapy Systems (CTS) Knockout DMEM F12 is a cGMP manufactured formula designed for culturing human embryonic stem cells and induced pluripotent stem cells. 
 

Customize your DMEM F12

If you require a custom DMEM F12 formulation, use our media customization services to select the DMEM F12 composition that is right for you, including custom media components, packaging, and QC testing.

GMP manufacturing

Gibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, helping to ensure the consistency, reliability, and high quality you can rely on.
 

Sustainable solutions

We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.
 

Resources and support

Resources

Gibco Media Selection Tool
Find the right Gibco media for your application.  Use this interactive tool to select media based upon culture type, components, additives, and format. 

 

Cell Culture Select Tool
Search your cell line of interest to find product recommendations and resources to help enable a successful cell culture.

 

Gibco Cell Culture Basics
Learn best practices and fundamentals of cell culture. Topics include aseptic technique and methods for the growth and maintenance of cells in culture.

 

Cell Culture Videos
Explore this collection of cell culture videos providing valuable insights into techniques, resources, and the latest advancements in the field.

 

Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Support

Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

 

Need technical support? Contact our expert team for technical and application support of Laboratory Products.

 

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