Mammalian Cell-Free Protein Expression | Thermo Fisher Scientific

We have several optimized cell-free expression systems for the rapid synthesis of recombinant proteins utilizing rabbit reticulocyte, or HeLa-based lysate systems. These mammalian systems can produce full-length and functionally active proteins, with the HeLa systems typically expressing higher protein yields than rabbit reticulocyte system.

More choices—a variety of mammalian expression systems to accommodate different vectors, tags, yield requirements, functionality needs and cost considerations
Fast—proteins can be expressed in as little as 90 minutes
Easy-to-use—kits include all reagents and accessories needed for protein expression experiments
High performance—kits are designed to maximize protein yield and/or function compared to similar products from other vendors

Featured mammalian cell-free protein expression products

1-Step Human High Yield IVT Kits

Multiple affinity tags to purify expressed protein

T7 cell-free expression vectors

Choose the right mammalian protein expression system for your experiment

	Retic Lysate IVT Kit	1-Step Human Coupled IVT Kit	1-Step Human High Yield IVT Kit
Source	Rabbit reticulocyte	HeLa lysate	HeLa lysate
Yield	Low	Medium	High
Protein modifications	Limited glycosylation	Glycosylation and phosphorylation	Glycosylation and phosphorylation
Reaction time	90 min	90 min to 6 hr	6–24 hr
Comments	Flexible system	First human-based system	Higher protein yield per reaction
Catalog #	60 rxn: AM1200, 60 rxn w/manual AM1200M	DNA: 88881, 88882	Mini: 88890, 88891 Maxi: 88892

T7 cell-free expression vectors

Thermo Scientific T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized for use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes, into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection.

Ordering information for T7 cell-free expression vectors

Resources

For Research Use Only. Not for use in diagnostic procedures.