Thermo Scientific Pierce Calibration Solutions for mass spectrometry are ready-to-use liquid formulations that can quickly calibrate Thermo Scientific LC-MS instrumentation. In addition to calibration solutions, standards for sensitivity assessment, determination of digestion efficiency, or as a control for sample analysis are available. Our high purity, validated mobile phases and acidic ion-pairing agents are essential for achieving effective and reproducible liquid chromatography (LC) separation of peptides for electrospray ionization (ESI) MS. 

  • Optimized—products developed to maximize LC-MS instrument performance
  • Convenient—calibration solutions, standards and solvent blends require little or no preparation
  • High purity—all reagents provided in non-leachable containers
  • Validated—all products have been manufactured in a ISO 9001 certified facility and fully tested using Thermo Scientific Mass Spectrometers.
  • Long shelf life—products stable for a minimum of 1 year if stored properly
 

Featured calibration solutions, standards & solvents categories

The Thermo Scientific Pierce Standards are designed for a variety of applications, including sensitivity assessment, determination of digestion efficiency, as controls for simple to complex sample analysis, or for intact protein analysis.

Mixtures of highly purified ionizable molecules specifically designed for positive calibration of Thermo Scientific ion trap, orbitrap and triple quadrupole instruments

The most commonly used solvents or solvent blends include LC/MS grade water and acetonitrile, with ion pairing agents such as trifluoroacetic acid (TFA), formic acid (FA) or heptafluorobuteric acid (HFBA)

Featured data

Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific Vantage triple quadrupole mass spectrometer.

Example selected reaction monitoring (SRM) of 500 fg reserpine on a Thermo Scientific Vantage triple quadrupole mass spectrometer. Chromatogram of three 5 μL loop injections of 100 fg/μL Thermo Scientific Pierce Reserpine on a Thermo Scientific Hypersil GOLD aQ 2.1x20 mm Javelin column with isocratic 300 μL/min flow of 70% methanol: 30% water: 0.05% formic acid (upper panel). The SRM transitions monitored were 609.3-195.1 and 609.3-448.2 m/z with a 0.2 FWHM Q1 peak width (lower panel).

Thermo Scientific™ Pierce™ HeLa digest standard base peak chromatogram.

Thermo Scientific Pierce HeLa digest standard base peak chromatogram. Chromatogram of 200 ng Pierce HeLa Protein Digest Standard separated using a Thermo Scientific Acclaim PepMap 100 3 μm x 75 μm x 15 cm column (Product # 160321) with a 2-35% gradient (A: 0.1% FA in water, B: 0.1% FA in 100% acetonitrile) at 300 nL/min for 120 minutes and detected on a Thermo Scientific LTQ Orbitrap XL Mass Spectrometer.

Example SRM of reserpine
Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific Vantage triple quadrupole mass spectrometer.

Example selected reaction monitoring (SRM) of 500 fg reserpine on a Thermo Scientific Vantage triple quadrupole mass spectrometer. Chromatogram of three 5 μL loop injections of 100 fg/μL Thermo Scientific Pierce Reserpine on a Thermo Scientific Hypersil GOLD aQ 2.1x20 mm Javelin column with isocratic 300 μL/min flow of 70% methanol: 30% water: 0.05% formic acid (upper panel). The SRM transitions monitored were 609.3-195.1 and 609.3-448.2 m/z with a 0.2 FWHM Q1 peak width (lower panel).

HeLa digest standard base peak
Thermo Scientific™ Pierce™ HeLa digest standard base peak chromatogram.

Thermo Scientific Pierce HeLa digest standard base peak chromatogram. Chromatogram of 200 ng Pierce HeLa Protein Digest Standard separated using a Thermo Scientific Acclaim PepMap 100 3 μm x 75 μm x 15 cm column (Product # 160321) with a 2-35% gradient (A: 0.1% FA in water, B: 0.1% FA in 100% acetonitrile) at 300 nL/min for 120 minutes and detected on a Thermo Scientific LTQ Orbitrap XL Mass Spectrometer.

Resources

For Research Use Only. Not for use in diagnostic procedures.