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On Demand Webinar: CO2 Incubators and Cell Culture
Culturing cells at physiological oxygen concentration provides biologically relevant results
For many decades, cell lines have been cultured in standard CO2 incubators at “normal” atmospheric oxygen concentrations of about 21%. But oxygen concentrations in the human or animal body, which affects metabolism, proliferation, differentiation, disease progression and more, are much lower, varying from as low as 1-2 % up to about 12%, depending on the tissue microenvironment.
Controlling oxygen in vitro is increasingly important in cancer and other disease modeling, drug discovery, toxicity testing, regenerative tissue development and biopharmaceutical production. Increasingly, work involving cell types that are more physiologically relevant, including primary cells, stem cells and 3D cultures, and culturing these cells under hypoxic conditions is critical for results that are predictive of the in vivo state.
In this presentation, Mary Kay Bates, our Senior Global Cell Culture Scientist, covers the following topics:
- Review history and advances in understanding oxygen concentration in vivo and how hypoxia-inducible factor-1 (HIF-1) modulates the cell’s response;
- Present examples demonstrating the effects of hypoxia on cultured cells including better cell growth, less differentiation and less oxidative damage;
- Explain how “tri-gas” CO2 incubators provide a physiological oxygen environment.
Mary Kay Bates
Mary Kay Bates is a Senior Global Cell Culture Scientist with Thermo Fisher Scientific, where she provides cell culture expertise to colleagues and customers. Her knowledge is based on twenty years of experience in academic and industrial cell and molecular biology labs focusing on cancer and gene therapy, authoring several publications. Mary Kay holds an M.S. in microbiology from the University of Wisconsin-Madison
In this presentation, Mary Kay Bates, our Senior Global Cell Culture Scientist, covers the following topics:
- Review history and advances in understanding oxygen concentration in vivo and how hypoxia-inducible factor-1 (HIF-1) modulates the cell’s response;
- Present examples demonstrating the effects of hypoxia on cultured cells including better cell growth, less differentiation and less oxidative damage;
- Explain how “tri-gas” CO2 incubators provide a physiological oxygen environment.
Mary Kay Bates
Mary Kay Bates is a Senior Global Cell Culture Scientist with Thermo Fisher Scientific, where she provides cell culture expertise to colleagues and customers. Her knowledge is based on twenty years of experience in academic and industrial cell and molecular biology labs focusing on cancer and gene therapy, authoring several publications. Mary Kay holds an M.S. in microbiology from the University of Wisconsin-Madison
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