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Validating Routine Vibrio Testing in Modern Food Testing Laboratory
Free food microbiology on-demand webinar
We would like to invite the food microbiology testing laboratories community to view our webinar and discussions on the detection of Vibrio species and uncovers validation components in validating a testing method.
Who should view this webinar:
QC Manager | QC Assistant | Lab Manager | Lab Technician | Microbiologist | QC Manager | Head of Microbiology and the broader community of food microbiology testing
During this webinar our experts will cover:
The subtle art in validating Vibrio routine methods for Vibrio testing
Speaker: Maria Nelson, AOAC Consultant
AOAC microbiological method validation is traditionally based on method comparison. Candidate method performance is compared to standard reference method procedures, such as those found in the US FDA Bacteriological Analytical Manual, USDA-FSIS Microbiology Laboratory Guidebook, and the International Organization for Standardization. Study designs can be qualitative and/or quantitative, depending on the candidate method result. But what do we do when the candidate method and reference method have different intentions and end results? This presentation will discuss the protocol developed to validate a multi-plex PCR method designed specifically to detect Vibrio cholerae, V. parahaemolyticus and V. vulnificus that will satisfy regulatory requirements worldwide, and the challenges faced when relying on cultural methods to evaluate PCR method performance.
An introduction to the Thermo ScientificTM SureTectTM Vibrio Cholerae, V. vulnificus and V. parahaemolyticus PCR Assay method
Speaker: Daniele Sohier, PhD, Senior Manager - Gloabl Scientific Affairs, Food Protection- Thermo Fisher
Vibrio species form part of the natural flora of the worldwide coastal marine environmental and have been isolated from a variety of different seafoods, including oysters, which are routinely eaten raw by the public and carry the risk of causing gastroenteritis in consumers. To reduce the risk of infection, a reliable and rapid detection method is required for this perishable food group.
Maria Nelson, AOAC Consultant
Maria Nelson has worked with the AOAC RI and AOAC INTERNATIONAL since 2003 as a technical consultant coordinating microbiology and chemistry method validation studies in thePerformance Tested MethodsSM(PTM) andOfficial Methods of AnalysisSM(OMA) programs. She also serves as a technical advisor for AOAC contract-related activities and integrated programs. Maria’s background is in microbiology and molecular biology method development.
Daniele Sohier (PhD)
Senior Manager - Global Scientific Affairs - Food Protection
Thermo Fisher Scientific
Daniele is coordinating the scientific interaction with research scientists, laboratory and food safety managers, as well as the relationship with food safety authorities and certification bodies. She is responsible for developing the global food certification strategies and ensuring the recognition of Thermo Fisher Scientific solutions in food protection.
Daniele has certainly coordinated more than 100 validation studies of alternative methods according to the ISO 16140 standard and AOAC Guidelines. Daniele is as a member of the Board of Director of AOAC International, and more recently she joined the Expert Review Panel on Microbiology Methods for Food and Environmental Surfaces and the Official Methods Board (OMB) of Directors. Daniele is an active member of both MicroVal general and technical committees. She is as well a member of the AFNOR committees.
Daniele is strongly involved in international standardization with AOAC and ISO, and she is used to convene working groups or projects.
She was the President of IAFP Europe in 2018 and has organized multiple international symposia and conferences. She has published more than 45 publications and run more than 75 international communications. Daniele has also been involved in more than 20 national and European R&D programs.
During this webinar our experts will cover:
The subtle art in validating Vibrio routine methods for Vibrio testing
Speaker: Maria Nelson, AOAC Consultant
AOAC microbiological method validation is traditionally based on method comparison. Candidate method performance is compared to standard reference method procedures, such as those found in the US FDA Bacteriological Analytical Manual, USDA-FSIS Microbiology Laboratory Guidebook, and the International Organization for Standardization. Study designs can be qualitative and/or quantitative, depending on the candidate method result. But what do we do when the candidate method and reference method have different intentions and end results? This presentation will discuss the protocol developed to validate a multi-plex PCR method designed specifically to detect Vibrio cholerae, V. parahaemolyticus and V. vulnificus that will satisfy regulatory requirements worldwide, and the challenges faced when relying on cultural methods to evaluate PCR method performance.
An introduction to the Thermo ScientificTM SureTectTM Vibrio Cholerae, V. vulnificus and V. parahaemolyticus PCR Assay method
Speaker: Daniele Sohier, PhD, Senior Manager - Gloabl Scientific Affairs, Food Protection- Thermo Fisher
Vibrio species form part of the natural flora of the worldwide coastal marine environmental and have been isolated from a variety of different seafoods, including oysters, which are routinely eaten raw by the public and carry the risk of causing gastroenteritis in consumers. To reduce the risk of infection, a reliable and rapid detection method is required for this perishable food group.
Maria Nelson, AOAC Consultant
Maria Nelson has worked with the AOAC RI and AOAC INTERNATIONAL since 2003 as a technical consultant coordinating microbiology and chemistry method validation studies in thePerformance Tested MethodsSM(PTM) andOfficial Methods of AnalysisSM(OMA) programs. She also serves as a technical advisor for AOAC contract-related activities and integrated programs. Maria’s background is in microbiology and molecular biology method development.
Daniele Sohier (PhD)
Senior Manager - Global Scientific Affairs - Food Protection
Thermo Fisher Scientific
Daniele is coordinating the scientific interaction with research scientists, laboratory and food safety managers, as well as the relationship with food safety authorities and certification bodies. She is responsible for developing the global food certification strategies and ensuring the recognition of Thermo Fisher Scientific solutions in food protection.
Daniele has certainly coordinated more than 100 validation studies of alternative methods according to the ISO 16140 standard and AOAC Guidelines. Daniele is as a member of the Board of Director of AOAC International, and more recently she joined the Expert Review Panel on Microbiology Methods for Food and Environmental Surfaces and the Official Methods Board (OMB) of Directors. Daniele is an active member of both MicroVal general and technical committees. She is as well a member of the AFNOR committees.
Daniele is strongly involved in international standardization with AOAC and ISO, and she is used to convene working groups or projects.
She was the President of IAFP Europe in 2018 and has organized multiple international symposia and conferences. She has published more than 45 publications and run more than 75 international communications. Daniele has also been involved in more than 20 national and European R&D programs.
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