On-demand webinar

Single-cell proteomics by mass spectrometry can provide valuable insights into distinct cell states and signaling patterns present in a cell population. However, carrying out proteomics profiling from the limited amount of material encapsulated in an individual cell presents significant challenges.

Tremendous efforts have been made to optimise all aspects of single-cell proteomics by mass spectrometry, with the aim of minimising losses during sample preparation and maximising sensitivity of data acquisition. Nevertheless, continuous development is needed to increase both the quantifiable proteome depth per cell, the quantitative accuracy and precision of those measurements, and the number of cells measured per unit time.

This webcast will present recently-developed approaches, ranging from sample preparation and chromatographic separation to advanced data acquisition, data interpretation and visualisation methods. Advances in instrumentation, including the novel Orbitrap Astral MS, and how to use both TMTPro-labeled and label-free approaches, will be presented, showcasing the application of these methods to biomedical questions, and providing an overview of the current technological state of the field.

About the presenter

Dr. Erwin Schoof, Associate Professor, Technical University of Denmark
Dr. Erwin Schoof, Associate Professor, Department of Biotechnology and Biomedicine, Section for Protein Science and Biotherapeutics, Cell Diversity Lab, Technical University of Denmark

Learn how to

  • Prepare single cells for analysis by mass spectrometry
  • Improve the sensitivity and throughput of single-cell proteomics analysis
  • Implement both label-free and labeled quantitative approaches to single-cell proteomics

 
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