Protein sample evaluation is an important step in many protein workflows. The Thermo Scientific™ NanoDrop™ One microvolume spectrophotometer supports protein sample quantification with multiple preconfigured applications, including direct absorbance at A280, A205, or other wavelengths using the Thermo Scientific™ Pierce™ colorimetric assays (BCA, Rapid Gold BCA, Pierce 660, Bradford, etc.) and a proteins and labels application for quantitation of metalloproteins and fluorescently labeled proteins.
We will review what you need to consider when choosing a protein quantification method for your sample. Is the sample one purified protein or a mixture of proteins? Does your peptide contain tyrosine and tryptophan residues? Does the sample buffer absorb in the UV spectrum? How can co-purified DNA affect your protein sample measurement? What is a good blank/control for your sample? Answers to these questions and other key considerations will be discussed.
