Super Bright 780

Comparable to Brilliant Violet 786 conjugates

Invitrogen eBioscience Super Bright 780 antibody conjugates for flow cytometry provide:

Multiplexing with Super Bright 780 antibody conjugates

Data comparison

Figure 1. Fluorescence intensity comparison of Super Bright 780 conjugates and Brilliant Violet™ 786 conjugates. (A) Mouse splenocytes stained with anti-CD4 conjugated to Super Bright 780 (red, Cat. No. 78-0042-80) or Brilliant Violet 786 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD8a conjugated to Super Bright 780 (red, Cat. No. 78-0088-41) or Brilliant Violet™ 786 conjugate (gray), using the same concentration of antibody.

Figure 2. Fluorescence intensity comparison of Super Bright 780 conjugates and Brilliant Violet™ 786 conjugates. Human peripheral blood cells stained with anti-CD19 conjugated to (A) Brilliant Violet™ 786 or (B) Super Bright 780 (Cat. No. 78-0198-41) at the same concentration of antibody.

Figure 3. Fluorescence intensity comparison of Super Bright 780 conjugates to eFluor 450 and PE conjugates. Mouse splenocytes cells stained with anti-CD3e conjugated to (A) eFluor 450 (Cat. No. 48-0621-80), (B) PE (Cat. No. 12-0621-81) or (C) Super Bright 780 (Cat. No. 78-0621-80).

Antibodies
Multiplexing compatibilityBufferMultiplexing considerations
Multiplexing with 1 Super Bright antibody conjugateStandard buffers applicableNo special buffer required when only one Super Bright antibody conjugate is used in a panel
Multiplexing with 2 or more Super Bright antibody conjugatesSuper Bright Staining BufferSpecial staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions
Multiplexing with 1 or more Brilliant Violet™ antibody conjugatesSuper Bright Staining BufferSpecial staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted
Viability stains
Viability stain optionsProductMultiplexing considerations
FixableLIVE/DEAD fixable dead cell stain kitsNo compatibility concerns
Non-fixableSYTOX non-fixable dead cell stains

Ready Flow Ready-to-use viability reagents		No compatibility concerns

Compatible with all Ready Flow reagents for viability
Compensation beads
ProductMultiplexing considerations
UltraComp eBeads microspheresUltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates.
Intracellular staining buffers
Staining TargetProductMultiplexing considerations
Cytosolic staining
(cytokines)		Intracellular Fixation & Permeabilization Buffer SetNo compatibility concerns
Nuclear staining
(transcription factors) 		Foxp3/Transcription Factor Staining Buffer SetNo compatibility concerns
Staining performance and post-fixation stability

Figure 4. Staining performance and post-fixation stability. Human peripheral blood cells stained with CD8a antibody conjugated to Super Bright 780 (Cat. No. 78-0088-41) and analyzed immediately (red) or subjected to different fixation buffers per the recommended protocol: (A) eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00), (B) eBioscience IC Fixation Buffer (Cat. No. 00-8222-49), or (C) eBioscience IC Fixation Buffer followed by 30 minutes in 90% methanol and were analyzed at 30 min (blue), overnight (orange) or after 3 days (green).

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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

For Research Use Only. Not for use in diagnostic procedures.