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Fluoroskan, Fluoroskan FL and Luminoskan instruments are all equipped with powerful Thermo Scientific SkanIt Software, offering increased usability for setting up protocols and data analysis. Use verified protocols from the growing SkanIt Cloud Library to help save time and make your workflow more efficient. The Thermo Scientific Fluoroskan Microplate Fluorometer is a compact, robust fluorescence plate reader built to run variety of fluorometric and FRET assays with excellent performance. The Thermo Scientific Luminoskan Microplate Luminometer is a sensitive, DLReady certified luminescence plate reader built to perform superbly for a variety of luminescence applications, including flash, glow, and BRET assays. The DLReady-certified Thermo Scientific Fluoroskan FL Microplate Fluorometer and Luminometer is a dual-mode versatile workhorse for fluorometric and luminometric assays.
In addition to the common features of all Microplate Reader instruments, the Fluoroskan FL, Fluoroskan fluorometric, Luminoskan Microplate Readers offer:
Reagent dispensers: simultaneous dispense and read
Enables easy and accurate reagent addition simultaneously as the instrument reads—a critical feature for flash-type reactions. All Fluoroskan and Luminoskan models are offered with three options of associated reagent dispensers: two dispensers, one dispenser, or no dispensers.
Flexibility to read 6- to 384-well plates
Allows you to choose your sample format depending on your unique research and throughput needs.
Auto-calibration and self-diagnostics
Enables accurate fluorescence data with every run. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before your run.
High sensitivity for both top and bottom reading
Fiberless direct illumination optics for both top and bottom reading. Top reading is great for homogeneous, BRET and FRET assays, whereas bottom-reading becomes very important for non-homogeneous and cell-based assays. Bottom reading shortens the distance between the cell layer at the bottom of the well and the detector and minimizes any effect from the cell culture medium.
Dynamic range >6 decades
Reliably measure high and low signal samples with Fluoroskan and Luminoskan readers that provide both sensitivity and broad dynamic range in the same run.
Incubation and shaking
Incubate up to 45°C with orbital shaking.
Multipoint well reading
Read multiple locations in a single well by stipulating in SkanIt Software where in the well you want to read. Multipoint measurements on Thermo Scientific fluorescence plate readers can also be used as an alternative or quantitative complement to imaging fluorospot assays.
Technology |
Specification |
Fluoroskan |
Luminoskan |
Fluoroskan FL |
|
|
|||
Fluorometry |
Excitation wavelength range |
320–700 nm |
|
320–700 nm |
|
Emission wavelength range |
360–800 nm |
|
360–670 nm |
|
Excitation filters |
Up to 8 filters in the excitation filter wheel; 355 nm and 485 nm filters included as standard. Other filters available upon request. |
|
Up to 8 filters in the excitation filter wheel; 355 nm and 485 nm filters included as standard. Other filters available upon request. |
|
Emission filters |
Up to 8 filters in the emission filter wheel; 460 nm and 538 nm filters included as standard. Other filters available upon request |
|
Up to 6 filters in the emission filter wheel. 460 nm and 538 nm filters included as standard. Other filters available upon request. |
|
Sensitivity |
2 fmol fluorescein/well in a black 96-well plate |
|
2 fmol fluorescein/well in a black 96-well plate |
|
Dynamic range |
>6 decades |
|
>6 decades |
Luminometry |
Spectral range |
|
270–670 nm |
270–670 nm |
|
Sensitivity |
|
10 amol ATP/well using flash reaction, white 384-well plate |
40 amol ATP/well using flash reaction, white 384-well plate |
|
Dynamic range |
|
>6 decades |
>6 decades |
|
Luminometric filters |
|
Up to 6 luminometric filters in the filter wheel. All filters available upon request. |
|
General features |
Number of dispensers |
Up to 3* |
Up to 3* |
Up to 3* |
|
Dispensing volume |
4–995 μL in 1 μL increments |
4–995 μL in 1 μL increments |
4–995 μL in 1 μL increments |
|
Dispensing speed |
30 s, 96-well plate, 5 μl/well |
30 s, 96-well plate, 5 μl/well |
30 s, 96-well plate, 5 μl/well |
|
Plate types |
6- to 384-well plates |
6- to 384-well plates |
6- to 384-well plates |
|
Measurement speed |
18 s, 96-well plate 60 s, 384-well plate |
18 s, 96-well plate 60 s, 384-well plate |
18 s, 96-well plate 60 s, 384-well plate |
|
Wavelength selection |
Filters |
Filters |
Filters |
|
Light source |
Quartz-halogen lamp |
|
Quartz-halogen lamp |
|
Detector |
Photomultiplier tube |
Photomultiplier tube |
Photomultiplier tube |
|
Incubator |
From ambient + 3°C to 45°C, at ambient 25°C |
From ambient + 3°C to 45°C, at ambient 25°C |
From ambient + 3°C to 45°C, at ambient 25°C |
|
Plate shaking |
Orbital |
Orbital |
Orbital |
|
User interface |
PC software (SkanIt software) |
PC software (SkanIt software) |
PC software (SkanIt software) |
|
Computer interface |
USB 2.0 |
USB 2.0 |
USB 2.0 |
|
Dimensions (H x W x D) |
340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in. |
340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in. |
340 x 420 x 420 mm 13.4 x 16.5 x 16.5 in. |
|
Weight |
Basic unit 21 kg (46 lb) 2 optional dispensers add 2.4 kg |
Basic unit 20.5 kg (45 lb) 2 optional dispensers add 2.4 kg |
Basic unit 21 kg (46 lb) 2 optional dispensers add 2.4 kg |
For Research Use Only. Not for use in diagnostic procedures.