Varioskan LUX Multimode Microplate Reader | Thermo Fisher Scientific

This multimode microplate reader combines simplicity with top-of-the-line performance. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated dynamic range selection, which adjusts the optimal reading range based on signal intensities; and built-in safety controls, which alert you to potential errors before they happen.

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Features

In addition to the common features of all Microplate Reader instruments, the Varioskan LUX offers:

Automatic dynamic range selection

Designed to automatically select the optimal reading range based on the signal intensity in each well. This feature saves time and reduces the risk of loss of sensitivity or over-saturation of signals.

Integrated gas control

The ability to control O₂ and CO₂ levels in your microplate reader replaces the need for constant handling of plates between the incubator and reader, and helps to ensure that cells remain alive throughout the experiment.

Smart safety controls

Automatic safety checks designed to alert you of potential problems before they happen. This decreases the potential of a range of experimental errors, from user mistakes to power outages.

Flexible wavelength selection

The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology.

Available measurement technology configurations

All configurations available with top or top and bottom fluorescence intensity reading; none, one, or two dispensers; and optional integrated gas modules.

System configuration options

Absorbance and Fluorescence intensity

Absorbance, Fluorescence intensity, and Luminescence

Absorbance, Fluorescence intensity, Luminescence, and AlphaScreen

Absorbance, Fluorescence intensity, Luminescence, and Time-resolved fluorescence

Absorbance, Fluorescence intensity, Luminescence, Time-resolved fluorescence, and AlphaScreen

Videos and demos

Specifications

Category	Attribute	Description
Absorbance	Plate types	6-384 well plates
	Wavelength selection	Double monochromators
	Wavelength range	200–1,000 nm
	Light source	Xenon flash lamp
	Read out range	0–6 Abs
	Linear measurement range	0–4 Abs (96-well plate) at 450 nm, ± 2% 0–3 Abs (384-well plate) at 450 nm, ± 2%
	Accuracy	0.003 Abs or ± 2%, at 200–399 nm (0–2 Abs) 0.003 Abs or ± 1%, at 400–1,000 nm (0–3 Abs)
	Precision	SD <0.001 Abs or CV <0.5%, at 450 nm (0–3 Abs)
Fluorescence Intensity	Plate types	6 – 1536-well plates (top reading) 6 – 384-well plates (bottom reading)
	Wavelength selection	Double excitation and emission monochromators
	Excitation wavelength range	200–1,000 nm
	Emission wavelength range	270–840 nm
	Light source	Xenon flash lamp
	Sensitivity	Top reading: <0.4 fmol fluorescein/well (black 384-well plate) Bottom reading: <4 fmol fluorescein/well, (clear bottom black 384 square well plate)
	Dynamic range	Top reading >6 decades Bottom reading >5.5 decades
Time-resolved fluorescence	Plate types	6–1,536 well plates
	Wavelength selection	Filters (spectral scanning with double excitation and emission monochromators)
	Excitation wavelength range	Fixed to 334 nm (spectral scanning 200–840 nm)
	Emission wavelength range	400–700 nm (spectral scanning 270–840 nm)
	Light source	Xenon flash lamp
	Sensitivity	<1 amol Eu/well (white low volume 384 well plate)
	Dynamic range	>6 decades
Luminescence	Plate types	6–1536 well plates (spectral scanning 6-384 well plates)
	Wavelength selection	Direct or filters (spectral scanning with double monochromators)
	Wavelength range	360–670 nm
	Sensitivity	<7 amol ATP/well (white 384-well plate)
	Dynamic range	>7 decades
Alpha	Plate types	6-1536 well plates
	Wavelength selection	Filters
	Excitation wavelength range	Fixed to 680 nm
	Emission wavelength range	400–660 nm
	Light source	LED
	Sensitivity	<100 amol phosphotyrosine/well (white 384-well plate)
Dispensing	Plate types	6-384 well plates
	No. of dispensers	None, one or two
	Syringe size	1 mL (standard), 5 mL (optional)
	Dispensing volume	2–5,000 μl, in 1 µl increments (1 mL syringe) 5–25,000 μl, in 5 µl increments (5 mL syringe)
	Accuracy	<1 μl with 50 μl (0.4 mm tip) <0.2 μl with 5 μl (0.25 mm tip)
	Precision	<1 μl with 50 μl (0.4 mm tip) <0.25 μl with 5 μl (0.25 mm tip)
	Dead volume	Reagent loss <100 μl, Total tubing volume <800 μl
Incubator and Shaker	Temperature range	From ambient + 4 °C to 45 °C
Incubator and Shaker	Shaking type	Orbital
Integrated Gas Module	Measurement chamber O₂ concentration accuracy	±1.0% (37 °C, 1% O₂)
	Measurement chamber CO₂ concentration accuracy	±1.0%. (37 °C, 5% CO₂)
	Measurement chamber O₂ concentration	1-21%
	Measurement chamber CO₂ concentration	0.1-15%
	Gas concentration recovery time	10 minutes
General Features	Measurement modes	End-point, kinetic, spectra, multipoint and kinetic spectra
	Measurement speed	Reads a 96-well plate in 15 s, a 384-well plate in 45 s, and a 1,536-well plate in 135 s (minimum times)
	Interface	PC software (SkanIt software)
	Dimensions (D x W x H)	58 x 53 x 51 cm (23 x 21 x 20 in)
	Weight	53 - 60 kg, (117-132 lb), depending on configuration

For Research Use Only. Not for use in diagnostic procedures.